FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28756903
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGT6142082.135862822223937No Hit
ACCAGG5946602.0678861002521725No Hit
AACTCT4795331.66754048584439No Hit
ATTCAT4286551.49061600965862No Hit
TTCTAC3659101.272424920027028No Hit
ACTAGC3631031.2626637854570084No Hit
GGTAAT3606931.254283188979008No Hit
ATCCGG3574221.242908528780029No Hit
CCGTCG3352721.1658835445527636No Hit
CGTTGC3176201.1045000221338162No Hit
CCATGA2989961.0397364417162724No Hit
TAGAGC2816320.9793544179635756No Hit
CGAATT2700270.9389988901099677No Hit
AGTGCA2699170.9386163732582747No Hit
AATCGG2694150.936870705444185No Hit
CGTACT2491560.8664215336401142No Hit
CAAGGA2464810.8571194192921261No Hit
GCATAA2425080.8433036060941611No Hit
TCTTCA2384950.8293486958592169No Hit
CCACTT2370800.8244281381760756No Hit
GGTACC2364290.822164333899238No Hit
AACATA2356320.8193928254374263No Hit
AACTAG2329740.8101498273301545No Hit
CTATTA2303590.8010563585376353No Hit
CCTTAT2213430.7697038864025101No Hit
TACGAA2148200.7470206370971172No Hit
AGACGG2131730.741293316599496No Hit
CCTGCT2045230.7112135823527311No Hit
TGTGAT2015410.7008438982459273No Hit
ACCTGC2010390.6990982304318375No Hit
GCACAT1993610.6932631097305576No Hit
GATCCT1982570.68942403150993No Hit
GTCTCA1930550.6713344618507772No Hit
TACGGC1905580.6626513293173468No Hit
CAGGAC1892480.6580959013562762No Hit
CCTCGG1880250.6538430094506352No Hit
GCTGTC1855060.6450833735468663No Hit
TGGCAT1846020.641939780511135No Hit
ACAGCC1813850.630752901312078No Hit
TTCTGT1809660.6292958598497203No Hit
AGGCTT1794010.6238536882779067No Hit
GCTCAA1786090.6210995669457173No Hit
ATGGCC1781220.6194060605204948No Hit
TTAGTA1780040.618995724261406No Hit
GTACTT1779140.6186827559282027No Hit
TCGTGG1769510.615333994762927No Hit
GAGTCG1747220.6075828123772578No Hit
GTGGTG1738560.6045713615266567No Hit
ATCCAC1735100.6033681721567862No Hit
GTTGGC1716240.5968097468632141No Hit
CTGGAG1714070.5960551454376015No Hit
AGTCAA1689310.5874450388485853No Hit
GTTACA1676290.5829174302949105No Hit
TATTCT1671770.5813456337770447No Hit
CACCTA1665320.5791026940557542No Hit
CATGAG1662000.5779481886488264No Hit
CACATT1632360.5676410982086632No Hit
TAAGTC1628540.5663127215055113No Hit
GGACTA1622310.5641462851545592No Hit
AAGCAT1603650.5576574083794767No Hit
TCCTCC1588330.552329991863171No Hit
TATTGG1588200.5522847853261528No Hit
CATTAA1588060.5522361013632101No Hit
ACTAAT1587950.5521978496780408No Hit
TCCAGA1585340.5512902415117511No Hit
AGGACT1583500.5506503951416465No Hit
TTCCAT1582400.5502678782899536No Hit
AGCCTA1577400.5485291653277128No Hit
GACCAT1545270.5373561958323537No Hit
GAAGAT1540500.535697463666376No Hit
CGTCAG1536640.5343551772595262No Hit
GAGTGA1535130.5338300859449294No Hit
TACAGT1533450.5332458783896166No Hit
GAGCAC1531900.5327068773713219No Hit
AACCTT1506720.5239507188934776No Hit
GTAAGA1505020.5233595564863157No Hit
CGAGAG1484050.5160673943226779No Hit
CAGTAG1482940.5156814000450605No Hit
GCTTCT1479980.514652081971414No Hit
CTGAGG1477870.5139183451013484No Hit
AGATGC1477690.5138557514347077No Hit
TGCATA1477030.513626241323692No Hit
GAGATA1467510.5103157318435856No Hit
GACCGA1446050.5028531758096483No Hit
TATAAG1426310.4959887370347217No Hit
TATGGT1420740.4940518107947855No Hit
TCGGAC1395760.48536520083543067No Hit
ACCGCT1390230.4834421842991925No Hit
CATAAC1389200.48308400942897084No Hit
GGAGGC1385990.48196775570721223No Hit
ATTGCT1382120.480621991874438No Hit
TGTGTG1381670.48046550770783625No Hit
AATTGC1381670.48046550770783625No Hit
AGTAGG1367660.47559363398763765No Hit
GGTGGT1363430.474122682821582No Hit
CTGTAT1356580.4717406460633121No Hit
GACACG1342700.46691397888013186No Hit
AATGTA1337570.4651300593808728No Hit
CAAGCT1325640.4609814902529664No Hit
CGAAGC1320240.4591036802537464No Hit
ATCAGC1316860.4579283102912717No Hit
CGACAA1316570.45782746493946164No Hit
TTCACA1309250.4552819891627412No Hit
GGCGTT1296580.45087609051642313No Hit
ACACGA1280910.4454269640927606No Hit
TTCGTT1263010.43920237168793874No Hit
CCGACT1259310.4379157240958806No Hit
CTATGC1248370.4341114201344978No Hit
GGCCGT1243470.43240748143150187No Hit
CTTAGC1233070.42879095847004106No Hit
CTCCGC1222560.425136183823411No Hit
CGAGCC1221660.4248232154902077No Hit
CAGTCC1211830.4214049058064424No Hit
GACTAA1204660.41891159141858914No Hit
CGTGAA1200180.4173537046044214No Hit
TCTCTG1200160.41734674975257247No Hit
CTAGTT1193340.414975145272076No Hit
AACAAT1178280.40973814182980695No Hit
ACGTAA1176240.4090287469412127No Hit
ATTCGA1170270.40695272366429724No Hit
GGATTC1168060.4061842125349868No Hit
ATGCCG1164110.4048106292948166No Hit
GTACGC1142130.39716724711280627No Hit
TGATAA1125670.39144340404110967No Hit
ACGAAC1122330.3902819437823329No Hit
GGATCG1118410.38891879281993613No Hit
GGCCAG1114560.38757998383901077No Hit
AGGTGT1112420.38683581469117173No Hit
GTCGAG1098420.38196741839689763No Hit
TAATGA1090280.37913679369436964No Hit
TTGAAT1084280.37705033813968075No Hit
AGGTCA1084080.37698078962119114No Hit
ATTGAA1079840.375506361029211No Hit
TCATGT1026180.3568464935184432No Hit
CGTCTC1024380.3562205568520366No Hit
CTTCAC1012670.3521484910944687No Hit
AGAGGT1005090.3495126022437117No Hit
TCAAGC996420.3464976739671863No Hit
TACTCG993210.3453814202454277No Hit
GCGGCT988870.3438722173942027No Hit
GAGCCA988610.3437818043201662No Hit
GCCATA960250.3339198243983366No Hit
GATTCA947050.32932962217802103No Hit
TCCACG942420.32771957397498613No Hit
TGCGGA941630.32744485732695205No Hit
CTGTTC934280.32488894927245815No Hit
ATGTGA931260.3238387666432647No Hit
GCCTGG919130.31962064899686865No Hit
TCTCGC906970.31539209907269916No Hit
GAACCG901420.3134621276846119No Hit
CAGCCT895680.3114660852039596No Hit
ATACCT881450.3065177081134224No Hit
GCGTAT880510.30619083007652115No Hit
GACTTG871200.30295334654082884No Hit
GTCTGC870700.30277947524460475No Hit
CATACG864240.3005330580973897No Hit
AAGCGC847910.29485442156271136No Hit
GAGAAG831050.2889914814540356No Hit
CCAGCG811200.2820887909939398No Hit
CTACCG802550.2790808175692633No Hit
ACGCTA790190.2747827191266041No Hit
GCGCAG772170.26851639761068846No Hit
TTAGCG763490.26549799190823853No Hit
AAGGCG742260.25811541667056426No Hit
CGCTTG739370.2571104405783891No Hit
TGCGAC692520.24081870012219328No Hit
AGAACG685770.23847143762316825No Hit
CCGGAA661110.22989610529339682No Hit
AGTTCC633500.22029493231590344No Hit
CTCGTC622320.2164071701323331No Hit
GGCAGG549850.19120626445761563No Hit
GAATAC526290.1830134489795372No Hit
CCTGAC502170.1746258976496878No Hit
TGACAC462600.1608657232665145No Hit
NNNNNN440710.15325363791782448No Hit
AGTTAG408660.14210848782986124No Hit
AAGAGG389970.1356091787770053No Hit
CAACGG347090.1206979764128286No Hit
TCAATG297730.10353340204958789No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)