FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22632982
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAT3494121.543817778850352No Hit
ACTAGC2903441.2828358189831106No Hit
ATTCAT2890661.2771891923035152No Hit
ATGGCC2576111.1382105990275608No Hit
GAATAC2568561.134874759322479No Hit
AGGACT2522121.114356031388175No Hit
GAGCAC2397941.0594892003183671No Hit
TCAATG2372051.0480501420449146No Hit
GCTTCT2307041.0193265739353303No Hit
CGTTGC2199070.9716218569872941No Hit
CAGTAG2183940.964936922584925No Hit
GGCCAG2145740.9480588991764319No Hit
TGATAA1984460.8768000610790041No Hit
ATGCCG1939450.8569131544398348No Hit
AGACGG1908700.8433267874290714No Hit
GAAGAT1838010.812093607461889No Hit
AGCCTA1779770.78636124926004No Hit
CATTAA1750910.7736099467582309No Hit
GGCGTT1748760.7726600056501614No Hit
GAACCG1741950.769651122419485No Hit
GGCCGT1659110.7330496706090254No Hit
TCCAGA1656100.7317197530577279No Hit
TGTGTG1653000.7303500705298135No Hit
CCGACT1650620.7292985078148341No Hit
GGTAAT1603180.7083379468070093No Hit
CGTGAA1549140.6844612875139475No Hit
TGTGAT1526800.6745907366514938No Hit
AGTTAG1525800.6741489035779731No Hit
CTATTA1501330.6633372482689202No Hit
TAGAGC1482160.6548673082495271No Hit
CTTAGC1473250.6509305755644572No Hit
CGTACT1472470.650585945767111No Hit
GGTACC1461650.6458053119116164No Hit
TCTTCA1453350.6421380974013942No Hit
TACGGC1449310.6403530917843703No Hit
CTGTAT1443070.6375960534056008No Hit
AAGTTG1430490.6320377933407095No Hit
TTCTGT1419050.6269832229796322No Hit
GCCAAT1388890.613657537482246No Hit
TACTCG1387250.612932931241672No Hit
GACCGA1385810.6122966916158021No Hit
AACAAT1379900.6096854581512944No Hit
ACCAGG1376680.6082627556545576No Hit
TCGGAC1367840.6043569512846341No Hit
AACATA1358060.6000358238256011No Hit
ATTGAA1349550.5962758243699394No Hit
GACACG1344950.5942433922317439No Hit
GTCTCA1344460.5940268940257187No Hit
AATTGC1322320.5842447097779692No Hit
CAAGGA1305020.57660099760606No Hit
GCCATA1301400.5750015618799149No Hit
GTCGAG1289170.5695979433907561No Hit
TACGAA1285440.5679499060265236No Hit
TTAGGC1277190.5643047831699773No Hit
CCGTCG1276300.5639115517345439No Hit
TTCGTT1276050.5638010934661637No Hit
CAGCCT1269590.5609468518112196No Hit
CGATGT1264570.5587288497821453No Hit
GTGGTG1263670.5583312000159767No Hit
AACCTT1262000.5575933387831971No Hit
GGCTAC1257150.5554504483766214No Hit
TATAAG1251390.5529054898731418No Hit
ACTTGA1247880.5513546557850839No Hit
CGAGCC1239760.5477669712280954No Hit
ACTAAT1232870.5447227413515374No Hit
CCAGCG1227210.5422219661554099No Hit
CAGTCC1203530.5317593589744383No Hit
GAGAAG1201840.5310126610801882No Hit
CTCCGC1193630.5273852115465828No Hit
GTCTGC1182310.5223836611543278No Hit
TACAGT1179990.5213586084237596No Hit
CCTCGG1176170.5196708060829104No Hit
ACACGA1165150.5148018056127116No Hit
CGAATT1158570.511894543988945No Hit
TCGTGG1158150.5117089740980663No Hit
ATGTGA1141780.5044761666845314No Hit
CGTCTC1137540.5026027944528034No Hit
GCACAT1137090.5024039695697191No Hit
TCATGT1132320.5002964258090251No Hit
GATCAG1126540.49774263064407515No Hit
TTACGT1120860.4952330187864772No Hit
TGACCA1118780.494314005993554No Hit
CTGAGG1112400.4914951109844916No Hit
CTTCAC1110660.49072632143656547No Hit
ACAGCC1109490.4902093767405462No Hit
TAGCTT1102340.4870502702648727No Hit
ACAGTG1100400.4861931141022425No Hit
GCTCAA1094310.48350235068450104No Hit
CGCTTG1090580.4818543133202686No Hit
ATCAGC1076240.475518427045981No Hit
GTACTT1074000.4745287209612944No Hit
ATTGCT1070230.47286301027412114No Hit
AAGGCG1063230.4697701787594759No Hit
CTACCG1059160.46797191815024636No Hit
GACCAT1053920.4656567128449976No Hit
TTAGCG1045180.46179509178242617No Hit
TTCTAC1042380.4605579591765681No Hit
GCATAA1039680.45936500987806206No Hit
GTAAGA1031060.4555564087843131No Hit
CCTGCT1024060.45246357726966774No Hit
TCCACG1021510.45133690293218987No Hit
AACTCT1018980.4502190652561824No Hit
TTAGTA1018490.4500025670501571No Hit
AATGTA1017650.4496314272683997No Hit
AACTAG1015030.44847382461577534No Hit
CCACTT1009420.44599514107332383No Hit
CGAAGC1006930.4448949767202572No Hit
CAGATC1000060.44185958350516963No Hit
CAACGG999120.4414442604160601No Hit
CATGAG998260.4410642839728322No Hit
GGACTA997610.4407770924750437No Hit
GCTGTC991710.4381702773412713No Hit
TATGGT987060.43611575354939974No Hit
GCGCAG986250.4357578687598479No Hit
TTCCAT981040.43345591844680476No Hit
TGCGAC980370.4331598902875458No Hit
CGACAA976810.4315869645458119No Hit
TTGAAT970230.4286797029220453No Hit
ATTCGA959060.4237444274908185No Hit
CCATGA959010.42372233583714247No Hit
AGGTGT957660.4231258611878895No Hit
ATACCT955260.4220654618114396No Hit
TTCACA942040.41622442857949515No Hit
GAGTCG936290.4136838884067508No Hit
CCTGAC934900.413069740434557No Hit
CTAGTT926670.40943345423948113No Hit
AGTGCA924490.40847025813920584No Hit
AGAACG912720.40326988286386656No Hit
GTACGC911820.4028722330976979No Hit
AAGCGC904140.3994789550930584No Hit
CATACG897640.39660704011517356No Hit
TCCTCC895960.39586476055165865No Hit
CAGGAC891540.3939118583666969No Hit
TGACAC887110.39195453785099993No Hit
GAGATA883810.39049648870838144No Hit
GATCCT878380.3880973351191637No Hit
ATCCGG870570.3846466188149666No Hit
AGTTCC864840.3821149153036926No Hit
CTGGAG863510.38152727731591No Hit
GGATCG862750.38119148418003423No Hit
CATAAC852610.37671129681453375No Hit
GAGTGA833600.36831205008690415No Hit
CGAGAG830370.36688492925943206No Hit
TCTCGC829310.3664165862015001No Hit
AGTAGG827950.36581569322151186No Hit
GTTGGC821650.3630321448583311No Hit
GACTTG811980.35875961903738535No Hit
GTTACA800910.35386852691351056No Hit
AAGAGG793850.350749185414454No Hit
CTGTTC792730.35025433237211073No Hit
TATTGG789090.34864605998449516No Hit
GATTCA788570.3484163067862644No Hit
GGCAGG784500.3466180461770349No Hit
GGTGGT784390.3465694445389476No Hit
AGATGC779970.34461654235398587No Hit
AGGTCA779630.3444663191089888No Hit
GACTAA765690.33830716606410943No Hit
CCGCTC758520.33513922292696563No Hit
TGCATA756780.3343704333790395No Hit
CAAGCT753660.33299191418965474No Hit
AAGCAT749080.33096831871292964No Hit
CACATT743150.32834824858695155No Hit
TGCGGA735320.32488869562128403No Hit
AATCGG733830.3242303643417381No Hit
TAAGTC724600.3201522450731415No Hit
ACCTGC717870.3171787084883468No Hit
CGTCAG715620.3161845840729251No Hit
CCGGAA715550.31615365575777865No Hit
GAGCCA714930.31587971925219577No Hit
ACGTAA707200.3124643495938803No Hit
TAATGA701530.3099591560670176No Hit
TATTCT684990.30265123703098423No Hit
GCCTGG678120.2996158438158966No Hit
AGTCAA673110.29740226011755766No Hit
GGATTC667790.29505170816642723No Hit
AGAGGT662610.29276301284558964No Hit
ACGAAC656240.28994853616726246No Hit
ACGCTA647190.28594994685189956No Hit
TGGCAT636870.2813902295331654No Hit
ACCGCT632810.2795963872546711No Hit
AGGCTT612650.2706890324924926No Hit
TCAAGC603590.26668602484639453No Hit
CACCTA597590.26403502640527No Hit
GGAGGC597540.26401293475159393No Hit
CTCGTC561960.24829251399572536No Hit
TCTCTG553660.24462529948550307No Hit
GCGGCT538460.23790943676798754No Hit
GCGTAT533610.23576654636141187No Hit
ATCCAC510210.22542765244102614No Hit
CTATGC455500.2012549649887054No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)