FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18797381
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGC6638593.5316568834775444No Hit
AGGACT5796833.083849819291315No Hit
CCTTAT5675653.019383391760799No Hit
GAGCAC5518222.9356323628275662No Hit
TCAATG5460832.905101513875789No Hit
GCTTCT5338702.840129696791271No Hit
CGTTGC5081672.703392563038436No Hit
ATGGCC4198432.233518594957457No Hit
GAATAC4143602.204349637856465No Hit
CATTAA4085672.1735315148424137No Hit
TGTGTG3906642.0782895234181824No Hit
CAGTAG3535681.8809428824153749No Hit
GGCCAG3508611.8665419400713323No Hit
AAGTTG3351201.7828015509181838No Hit
GACCGA3242551.725000945610455No Hit
AGACGG3166541.6845644614002346No Hit
ATGCCG3159191.6806543422192697No Hit
TTCGTT2985291.588141454386651No Hit
AGCCTA2912041.5491732598280579No Hit
GGCGTT2889951.5374216227249957No Hit
CCGACT2720381.4472122472806186No Hit
AATGTA2359191.2550631388489706No Hit
AACAAT2261411.2030452540170358No Hit
GAAGAT1860700.9898719401388948No Hit
CTATTA1549340.8242318437871744No Hit
GGTACC1491550.7934881992337124No Hit
GGTGGT1293990.688388451561417No Hit
CGAGCC1276700.6791903616785764No Hit
CGTGAA1213180.6453984201309746No Hit
ACACGA1193650.6350086748786972No Hit
TACGGC1182990.629337672093788No Hit
TCCACG1048690.557891548827999No Hit
ATTCAT1006320.5353511747194996No Hit
CAGCCT986660.5248922708966743No Hit
GCCAAT978040.5203065256803594No Hit
CCTCGG945680.5030913615040309No Hit
GAGAAG930350.4949359700694474No Hit
CAGGAC917060.48786583620345836No Hit
TTAGGC911570.48494521657032963No Hit
GATCCT908630.4833811688979438No Hit
GGATCG898430.4779548810549725No Hit
CGATGT896610.47698666106730503No Hit
AACCTT888530.47268818991326506No Hit
ACAGCC882380.46941645753735584No Hit
GGCTAC870620.4631602668478125No Hit
TTACGT866150.4607822759989809No Hit
ACTTGA864970.46015452897400977No Hit
CGAGAG862660.45892563437427797No Hit
ACCAGG855020.45486123838209164No Hit
AACATA850340.4523715298423754No Hit
GCATAA834820.44411506049699156No Hit
GACACG831790.4425031338142265No Hit
AAGGCG829480.4412742392144948No Hit
TATTGG820560.436528897296916No Hit
AACTAG809990.4309057735223859No Hit
CAACGG794200.4225056671458646No Hit
TGACCA789320.41990956080530584No Hit
TATGGT788680.4195690878426096No Hit
GATCAG780830.41539297415953846No Hit
ACAGTG771550.41045611620044303No Hit
TAGCTT766170.40759401535777773No Hit
TTGAAT750470.3992417879916357No Hit
GAGCCA745900.39681059824238285No Hit
GAGTCG744750.39619881088753794No Hit
GTACGC711090.37829206100573265No Hit
TGATAA701890.3733977621669742No Hit
CAGATC693460.3689130948614597No Hit
GAGTGA665630.3541078408742154No Hit
AGTAGG660180.3512085008012552No Hit
GTTACA643920.3425583595927539No Hit
GGCCGT627370.33375394157303084No Hit
CATGAG627220.3336741432223989No Hit
TCCAGA620250.32996617986303517No Hit
GCTGTC618470.32901923943553624No Hit
ATCCGG615020.32718387737100185No Hit
CATACG614470.3268912834186848No Hit
GGTAAT596880.31753359683458027No Hit
TCCTCC581020.30909625122776413No Hit
GAACCG574140.30543616687877956No Hit
ACGTAA569510.3029730577892739No Hit
CTGTTC561910.2989299413572561No Hit
CGTCAG560790.29833411367253765No Hit
TAGAGC550380.2927961081386817No Hit
CTTAGC548630.2918651273813091No Hit
TACTCG540740.28766773413806956No Hit
TGCATA538240.2863377616275374No Hit
AAGCAT527090.2804060842305638No Hit
TAAGTC517520.2753149494602466No Hit
TTCTGT516500.2747723206759495No Hit
GTTGGC514880.27391049848912463No Hit
ACGCTA507220.26983546271685405No Hit
CTGTAT507190.26981950304672764No Hit
TAATGA504380.26832461394488943No Hit
GTCTCA497360.2645900511353151No Hit
AGTTAG491500.26147259557062763No Hit
CAAGGA486260.25868497318855216No Hit
GTCGAG485610.2583391803358138No Hit
CCGCTC484460.257727392980969No Hit
GACTAA484290.2576369548502528No Hit
GGAGGC483270.25709432606595567No Hit
ATTGAA478740.25468441587687135No Hit
TGCGGA471620.2508966541668757No Hit
CTCCGC465280.24752384388016607No Hit
TGTGAT464160.24692801619544766No Hit
ACGAAC461590.24556080445462056No Hit
TGGCAT461420.2454703663239044No Hit
TATAAG457310.24328389151658947No Hit
TCGGAC454520.24179964219483555No Hit
CGTCTC441450.2348465459097733No Hit
TACAGT440610.23439967514623447No Hit
CTCGTC440150.23415496020429655No Hit
ACTAAT438770.23342081537848278No Hit
TCTCTG434300.23104282452965125No Hit
CTTCAC430280.2289042287327155No Hit
TACGAA429480.22847863752934516No Hit
CGTACT424380.22576549360785952No Hit
TCAAGC422000.2244993597778329No Hit
GCCATA421710.22434508296661115No Hit
CCGTCG421700.22433976307656903No Hit
TCTTCA421380.2241695265952209No Hit
GTCTGC417840.22228628552030733No Hit
TTAGCG403950.2148969582517905No Hit
GTACTT403440.21462564385964192No Hit
AATTGC401970.213843620023449No Hit
ATCAGC395920.2106250865479611No Hit
CAGTCC389240.2070713999998191No Hit
GACCAT385520.20509240090414724No Hit
AACTCT380290.20231009841211392No Hit
GTGGTG377400.2007726501899387No Hit
AGGTGT375220.1996129141607546No Hit
CCTGAC359420.19120748789419123No Hit
TCGTGG358220.19056910108913577No Hit
CCAGCG354930.18881885726527542No Hit
ATACCT354910.18880821748519114No Hit
GCGGCT348300.18529177016734405No Hit
TGACAC347680.18496193698473207No Hit
GCACAT346520.18434482973984515No Hit
AAGCGC345630.18387135952609568No Hit
CTAGTT343990.18299889755918655No Hit
GCGTAT340760.181280573075579No Hit
CGCTTG338170.17990272155466763No Hit
AGTTCC337540.17956756848201352No Hit
TCATGT334160.17776944564777403No Hit
ATGTGA333950.1776577279568893No Hit
TTCCAT332870.1770831798323394No Hit
ATTGCT332490.17688102401073852No Hit
CGACAA329560.1753222962283948No Hit
CTATGC328400.17470518898350787No Hit
TCTCGC327870.17442323481127503No Hit
CGAAGC326960.1739391248174413No Hit
CTGAGG326200.17353481317423955No Hit
CGAATT322020.17131109913662973No Hit
CTACCG317850.16909270498906204No Hit
GCTCAA317500.16890650883758754No Hit
CCTGCT316470.16835856016324827No Hit
TTCTAC311980.16596992953433246No Hit
TTAGTA310990.16544326042016172No Hit
AGTGCA307530.16360257846558518No Hit
CCACTT304360.16191617332223038No Hit
GGCAGG303370.16138950420805961No Hit
ATTCGA296320.15763898172835886No Hit
TGCGAC295360.1571282722843145No Hit
CCATGA292810.15577170032357168No Hit
GTAAGA286910.15263296519871572No Hit
TTCACA281740.14988258204693516No Hit
GGACTA281500.14975490468592406No Hit
GCGCAG280210.14906863887048946No Hit
CATAAC276100.14688216406317456No Hit
AGAACG271060.14420093948194168No Hit
GACTTG267030.14205702379496377No Hit
AGAGGT260780.13873209251863333No Hit
AAGAGG247320.13157152052192803No Hit
AGATGC247150.13148108239121184No Hit
GAGATA245900.13081609613594575No Hit
GATTCA245860.13079481657577724No Hit
CAAGCT241340.12839022627673505No Hit
CTGGAG238190.12671446091346447No Hit
AGGTCA234130.12455458555636022No Hit
ACCTGC231490.12315013458523824No Hit
GCCTGG211330.11242523626030668No Hit
AATCGG208350.11083990902775233No Hit
CACATT207950.11062711342606718No Hit
TATTCT203750.1083927596083731No Hit
CCGGAA201080.10697234896712472No Hit
AGGCTT197560.10509974767229542No Hit
CACCTA196290.10442412163694506No Hit
AGTCAA192560.10243980265123105No Hit
GGATTC190310.10124282739175207No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)