FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20779032
Sequences flagged as poor quality0
Sequence length6
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NCTTAT1805880.8690876456612608No Hit
NTTCAT1502260.72296919317512No Hit
NCTAGC1484270.7143114270193145No Hit
CCTTAT1408470.6778323456068598No Hit
NAATAC1306880.6289417139354712No Hit
NTGGCC1298350.6248366141406394No Hit
NGGACT1286780.6192685010543321No Hit
NCTTCT1232800.5932903900431935No Hit
NAGCAC1223180.5886607229826684No Hit
NCAATG1207070.5809077150465911No Hit
ACTAGC1169620.5628847388078521No Hit
ATTCAT1160320.5584090731464295No Hit
NGTTGC1119750.538884583266439No Hit
NAGTAG1109810.5341009148068111No Hit
NGCCAG1089480.5243170134200669No Hit
GAATAC1026390.4939546750782231No Hit
ATGGCC1025220.4933916074627538No Hit
AGGACT1016080.4889929425008826No Hit
NGATAA1013760.4878764323573879No Hit
NTGCCG972990.4682556916029582No Hit
GAGCAC964700.46426609285745357No Hit
TCAATG961590.46276939175992415No Hit
NGACGG956400.46027168156822706No Hit
NAAGAT938210.45151766453798237No Hit
GCTTCT918360.44196476524989226No Hit
NGCCTA905610.4358287720043936No Hit
NGCGTT890600.4286051438777322No Hit
NATTAA887470.4270988176927587No Hit
NAACCG884450.4256454294887269No Hit
CAGTAG882690.424798421793662No Hit
CGTTGC877360.42223333598985746No Hit
NGCCGT849010.4085897745381017No Hit
GGCCAG847800.4080074567477446No Hit
NCCAGA844390.40636637933855635No Hit
NCGACT842310.4053653702443887No Hit
NGTGTG836340.4024922816423787No Hit
NGTAAT815170.39230412658299No Hit
TGATAA804440.38714026716932726No Hit
NGTGAA796300.38322285657965205No Hit
ATGCCG777220.3740405231581529No Hit
NGTGAT775240.3730876395012049No Hit
NGTTAG775210.373073201870039No Hit
NTATTA766320.3687948505012168No Hit
NGTACT760230.36586401137454333No Hit
AGACGG759930.3657196350628846No Hit
NTTAGC757030.36432399738351623No Hit
NAGAGC756550.36409299528486216No Hit
NCTTCA754060.3628946718980942No Hit
NGTACC745220.35864038324788183No Hit
GAAGAT738770.3555362925472178No Hit
NTGTAT734290.35338027295977986No Hit
NAGTTG732170.3523600136907244No Hit
NTCTGT727920.35031468260889154No Hit
NACGGC721460.34720577936450553No Hit
AGCCTA714870.34403431305173404No Hit
NCCAAT709040.3412286000618316No Hit
NACAAT708230.34083878402035284No Hit
NACCGA705730.33963564808986285No Hit
NACATA703710.33866351425802704No Hit
NACTCG701800.3377443184071327No Hit
CATTAA698860.33632943055287656No Hit
NCGGAC697430.33564123680063634No Hit
NTCTCA695060.3345006639385319No Hit
NCCAGG693130.33357184300019366No Hit
GGCGTT692340.3331916520461588No Hit
NTTGAA691700.3328836492479534No Hit
GAACCG690780.3324408952255331No Hit
NACACG681570.32800854245760824No Hit
NATTGC673920.3243269465103091No Hit
NCCATA669040.32197842517399267No Hit
NAAGGA667340.32116029274125957No Hit
TCCAGA666230.320626100388122No Hit
NTCGTT663040.3190908989408169No Hit
TGTGTG660110.31768082363028266No Hit
CCGACT659040.317165881452033No Hit
GGCCGT654900.3151734883511417No Hit
NACCTT654840.3151446130888099No Hit
NACGAA653660.31457673292961863No Hit
NAGCCT650320.31296934332648413No Hit
NTCGAG649060.3123629628175172No Hit
NGATGT648170.3119346464262628No Hit
NCGTCG648080.31189133353276516No Hit
GGTAAT647800.31175658230855025No Hit
NTAGGC644880.310351319541738No Hit
NCTTGA641940.30893643168748186No Hit
NGCTAC641400.30867655432649604No Hit
NATAAG638990.3075167312895038No Hit
NTGGTG637600.3068477877121513No Hit
NCTAAT636620.30637615842739935No Hit
NGAGCC634100.30516339740946546No Hit
CGTGAA627900.30217962030185047No Hit
NCAGCG623660.30013910176373954No Hit
TGTGAT618430.2976221413971546No Hit
NAGTCC612800.2949126792816913No Hit
NAGAAG612110.29458061376487604No Hit
NTCTGC611720.2943929245597196No Hit
AGTTAG611500.2942870485978365No Hit
NTCCGC606890.2920684659420131No Hit
CTATTA604690.29100970632318196No Hit
TAGAGC603700.29053326449470795No Hit
NACAGT599920.28871412296780713No Hit
NGAATT599580.2885504964812605No Hit
CGTACT596300.2869719821404577No Hit
NCACGA595880.28676985530413546No Hit
NCTCGG595510.286591791186423No Hit
CTTAGC592740.2852587165754401No Hit
TCTTCA591540.2846812113288049No Hit
NCGTGG586170.2820968753501126No Hit
CTGTAT584920.2814953073848676No Hit
NCACAT584750.2814134941415943No Hit
NGTCTC583250.28069161258330033No Hit
NTGTGA582530.2803451094353192No Hit
GGTACC580000.27912753587366346No Hit
NATCAG576920.2776452724072998No Hit
NCATGT576580.2774816459207532No Hit
NTACGT575550.27698595391739134No Hit
AAGTTG574880.27666351348802004No Hit
TTCTGT571460.2750176235351098No Hit
NTGAGG568500.27359311059340974No Hit
NGACCA568390.27354017261246816No Hit
NCAGCC567870.2732899203389263No Hit
NAGCTT566460.27261135167413No Hit
NTTCAC566450.272606539130408No Hit
NTACTT565770.2722792861573147No Hit
NCAGTG564150.2714996540743573No Hit
NCTCAA562980.27093658645888796No Hit
TACTCG561110.2700366407828815No Hit
AACAAT557990.2685351271416301No Hit
NGCTTG556390.26776512014611653No Hit
AACATA555970.2675629933097942No Hit
NTTGCT555870.2675148678725746No Hit
ACCAGG552530.26590747826944006No Hit
GCCAAT552420.26585454028849853No Hit
NTCAGC550630.26499309496226775No Hit
TACGGC549100.2642567757728079No Hit
TCGGAC547820.2636407701763971No Hit
GACCGA543380.26150400076384694No Hit
NTACCG542320.2609938711293192No Hit
GTCTCA541740.26071474359344554No Hit
NTCTAC541670.2606810557873918No Hit
NACCAT539410.25959342090622894No Hit
ATTGAA538970.25938166898246273No Hit
GACACG538870.25933354354524313No Hit
NTAGCG534490.2572256493950248No Hit
NAGGCG532720.25637382915623785No Hit
CAAGGA529050.25460762561027867No Hit
NACTCT528800.2544873120172297No Hit
AATTGC528110.25415524650041443No Hit
NCTGCT527830.2540204952761996No Hit
NCATAA526650.25345261511700834No Hit
NTAAGA524820.2525719196158897No Hit
NTAGTA522400.2514072840351755No Hit
NATGTA520460.25047365055311527No Hit
TTCGTT517900.2492416393602936No Hit
NACTAG517490.24904432506769325No Hit
NCCACG517240.24892401147464427No Hit
NGAAGC517050.24883257314392704No Hit
NCACTT516830.2487266971820439No Hit
GCCATA516260.24845238218989218No Hit
TACGAA515980.24831763096567736No Hit
GTCGAG515900.24827913061590162No Hit
NGACTA512040.24642148873922518No Hit
NAGATC510970.2459065465609755No Hit
NTCCAT510700.2457766078804826No Hit
NAACGG509950.24541566710133564No Hit
AACCTT509940.2454108545576137No Hit
CCGTCG509910.24539641692644779No Hit
CAGCCT509220.24506435140963256No Hit
CGATGT509180.2450451012347447No Hit
NCTGTC508990.24495366290402748No Hit
TATAAG508430.24468416045559774No Hit
NATGAG508140.24454459668766093No Hit
GTGGTG507260.24412109284012842No Hit
NGCGAC505120.24309120848362906No Hit
NATGGT504070.2425858913928233No Hit
ACTTGA502580.2418688223782513No Hit
NCGCAG501020.24111806555762558No Hit
TTAGGC500370.2408052502156982No Hit
ACTAAT498050.2396887400722036No Hit
GGCTAC497880.23960692682893026No Hit
CGAGCC496880.23912567245673427No Hit
NTGAAT496370.23888023272691433No Hit
NTACCT495850.23862998045337241No Hit
NGACAA495730.23857222992870888No Hit
NCATGA492400.23696965286929633No Hit
NGGTGT492110.23683008910135947No Hit
CCAGCG489820.2357280165890307No Hit
NTCACA485140.23347574612715355No Hit
NTTCGA483890.23287417816190858No Hit
GAGAAG483720.23279236491863525No Hit
CAGTCC482590.23224854747805382No Hit
NCTGAC480900.23143522758904264No Hit
TACAGT478150.23011177806550373No Hit
NAGTCG477590.229842275617074No Hit
NGTGCA474960.22857657661819858No Hit
ACACGA473320.22778731944779718No Hit
NTAGTT473000.22763331804869447No Hit
GTCTGC472950.2276092553300847No Hit
CTCCGC471790.22705100025833735No Hit
CCTCGG471730.2270221249960056No Hit
CGAATT468100.22527517162493424No Hit
NCCTCC467460.22496716882672876No Hit
NGAACG466400.22445703919220106No Hit
TCGTGG464210.22340309211709186No Hit
NTACGC463610.22311433949377432No Hit
NAGGAC459380.22107863349938536No Hit
TCATGT458170.2204963157090282No Hit
ATGTGA457510.2201786878233789No Hit
NATACG456730.21980330941306603No Hit
NATCCT456570.21972630871351467No Hit
NAGCGC455590.21925467942876262No Hit
NGACAC454370.21866754909468353No Hit
CGTCTC453760.21837398392764396No Hit
TTACGT453570.21828254559692672No Hit
NAGATA453100.21805635604199464No Hit
GCACAT452980.21799860551733113No Hit
GATCAG452130.21758953930096453No Hit
TGACCA451230.21715641036598818No Hit
CTGAGG449820.21647784170119186No Hit
NTCCGG449550.21634790302069895No Hit
TAGCTT446010.2146442625431252No Hit
NGTTCC445640.2144661984254127No Hit
ACAGCC445360.21433144720119784No Hit
CTTCAC442820.21310906109582006No Hit
ACAGTG441970.21269999487945349No Hit
CGCTTG438320.21094341642093817No Hit
GCTCAA437790.21068835160367433No Hit
NGATCG436390.21001459548259993No Hit
NTGGAG436070.20986059408349722No Hit
NATAAC433090.20842645605435323No Hit
ATTGCT433020.20839276824829953No Hit
GTACTT431660.20773826230211304No Hit
AAGGCG431280.20755538564067852No Hit
ATCAGC430660.20725700792991703No Hit
TTCTAC425290.20467267195122466No Hit
NCTCGC424380.20423473047252635No Hit
CTACCG422580.2033684726025736No Hit
NAGTGA422260.20321447120347091No Hit
NGAGAG421100.20265621613172355No Hit
TTAGCG420080.2021653366720837No Hit
NGTAGG419560.20191508439854175No Hit
GACCAT419240.20176108299943904No Hit
NACTTG418750.20152526835706303No Hit
GTAAGA415390.19990825366648454No Hit
TCCACG413400.19895055746581455No Hit
AACTAG413220.19886393167881927No Hit
AATGTA412230.19838748985034532No Hit
NTTACA411500.19803617415864225No Hit
TTAGTA411380.19797842363397872No Hit
CCTGCT411210.19789661039070539No Hit
NTTGGC411040.19781479714743205No Hit
AACTCT410420.19751641943667056No Hit
GCATAA408460.19657316086716647No Hit
NTGTTC406730.19574059080326744No Hit
NATTCA406310.19553846396694513No Hit
CAGATC405200.1950042716138076No Hit
CAACGG405120.19496577126403195No Hit
CCACTT405120.19496577126403195No Hit
CATGAG403300.19408988830663526No Hit
NAGAGG403020.19395513708242038No Hit
NATTGG401660.19330063113623389No Hit
NGTGGT400680.19282900185148183No Hit
NGGTCA400280.19263650010260341No Hit
TGCGAC398670.19186168056336794No Hit
GGACTA398520.19178949240753854No Hit
GCTGTC398030.1915536777651625No Hit
NGATGC397200.19115423663623984No Hit
TATGGT396550.19084142129431245No Hit
CGAAGC396160.19065373208915604No Hit
NACTAA395910.19053341849610703No Hit
TTCCAT395680.19042272999050197No Hit
NGCAGG394880.1900377264927452No Hit
GCGCAG393650.18944578361494413No Hit
TTGAAT391420.18837258636494714No Hit
NAAGCT389630.18751114103871633No Hit
NGCATA389570.18748226577638458No Hit
CGACAA388290.18686626017997374No Hit
NACATT384780.18517705733356588No Hit
CCATGA384600.18509043154657062No Hit
NCGCTC384460.18502305593446317No Hit
AGGTGT384320.18495568032235574No Hit
ATTCGA384060.18483055418558478No Hit
NAGCAT383370.18449848866876956No Hit
ATACCT381100.18340604124388468No Hit
NATCGG377640.18174090111608665No Hit
CTAGTT376340.18111527043223186No Hit
TTCACA376050.18097570666429505No Hit
GAGTCG375820.18086501815868997No Hit
NGCGGA375120.1805281400981528No Hit
CCTGAC374590.18027307528088893No Hit
AGTGCA372510.17927206618672129No Hit
NAAGTC371320.1786993734838081No Hit
NGTCAG370440.17827586963627565No Hit
AGAACG369390.17777055254546986No Hit
NAGCCA367510.17686579432574145No Hit
NCCTGC365710.1759995364557887No Hit
NCGGAA364040.17519584165422142No Hit
NATTCT363290.17483490087507444No Hit
AAGCGC361870.17415151966655618No Hit
NAATGA361680.17406008133583895No Hit
TCCTCC360920.17369432801297No Hit
NCGTAA360130.1733141370589352No Hit
GTACGC359860.17318419837844226No Hit
TGACAC358890.17271738163741218No Hit
CAGGAC357900.17224093980893815No Hit
GAGATA356840.17173081017441044No Hit
ATCCGG351780.16929566305109883No Hit
GATCCT350660.16875665815423935No Hit
CATAAC348130.16753908459258351No Hit
CTGGAG347840.16739952082464668No Hit
NGTCAA345140.16610013401971757No Hit
NCCTGG344990.1660279458638882No Hit
AGTTCC344760.1659172573582831No Hit
NGATTC342980.16506062457577428No Hit
CATACG342770.16495956115761312No Hit
GGATCG341610.16440130608586578No Hit
NGAGGT339070.163178919980488No Hit
CGAGAG337340.162346349916589No Hit
GAGTGA334670.16106140074282574No Hit
NCGAAC334170.16082077355672775No Hit
TCTCGC334170.16082077355672775No Hit
AGTAGG333040.1602769561161463No Hit
NCGCTA332480.16000745366771657No Hit
NCCGCT328680.15817868705337187No Hit
GACTTG327850.15777924592444922No Hit
GTTGGC321670.15480509390427813No Hit
NGGCAT320760.15436715242557977No Hit
AAGAGG320000.15400139910271085No Hit
GTTACA319250.15364045832356388No Hit
TATTGG317660.15287526387177228No Hit
CTGTTC314820.1515085014547357No Hit
AGGTCA314400.1513063746184134No Hit
AGATGC313740.15098874673276408No Hit
GGTGGT313410.1508299327899394No Hit
GATTCA313230.15074330700294414No Hit
NGGCTT311750.1500310505320941No Hit
NACCTA311650.1499829250948745No Hit
TGCATA309090.1487509139020528No Hit
GGCAGG309000.14870760100855518No Hit
CAAGCT306350.14743227692223584No Hit
GACTAA302910.14577676188188168No Hit
NCAAGC302570.14561313539533507No Hit
NGAGGC301080.14489606638076305No Hit
CACATT300320.14453031305789413No Hit
TGCGGA298420.1436159297507218No Hit
AAGCAT298360.14358705448839001No Hit
CCGCTC296270.14258123285050045No Hit
AATCGG294770.1418593512922065No Hit
TAAGTC292640.14083427947942906No Hit
NTCGTC288490.13883707383481578No Hit
ACCTGC287640.13842800761844923No Hit
CGTCAG287310.13826919367562454No Hit
GAGCCA286780.13801412885836067No Hit
CCGGAA285990.13763393790432588No Hit
TAATGA283360.13636823890545044No Hit
NCTCTG282780.1360891113695768No Hit
ACGTAA281680.1355597315601612No Hit
NCGTAT278990.13426515729895405No Hit
NCGGCT276810.13321602276756683No Hit
TATTCT276490.13306202136846415No Hit
AGTCAA269720.1298039292686974No Hit
GCCTGG269260.12958255225748724No Hit
AGAGGT265040.12755165880682026No Hit
NTCCAC263790.1269500908415753No Hit
ACGAAC263240.1266854009368675No Hit
GGATTC263110.12662283786848202No Hit
ACCGCT257730.1240336893460677No Hit
ACGCTA257320.12383637505346737No Hit
TGGCAT257150.12375456181019405No Hit
AGGCTT246840.11879282923285357No Hit
CACCTA241480.11621330579788317No Hit
TCAAGC240090.11554436222053079No Hit
NTATGC236290.11371559560618609No Hit
GGAGGC230150.1107606937609028No Hit
CTCGTC225550.10854692364880136No Hit
TCTCTG222540.10709834798849148No Hit
GCGTAT213840.10291143495038653No Hit
GCGGCT213290.10264674504567874No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)