FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24479958
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACG5794802.367160924050605No Hit
CAGCCT4816661.96759324505377No Hit
GAGAAG4699111.919574371818775No Hit
AACCTT4666291.9061674860716673No Hit
CGTTGC4382341.79017464000551No Hit
CTGTTC4332371.769762023284517No Hit
GAGCAC4269541.744096129576693No Hit
TGCATA4044051.651984043436676No Hit
ACGAAC4012461.63907960953201No Hit
TTACGT3971921.6225191236030716No Hit
GCTTCT3738251.5270655284620995No Hit
GAAGAT3707851.5146472065025602No Hit
TCAATG3707801.5146267816309162No Hit
ACTAGC3537921.4452312377333327No Hit
ATCCGG3424741.3989974982800215No Hit
AAGGCG3365371.3747450056899608No Hit
CAGGAC3267671.3348348064976254No Hit
ACACGA3222991.3165831411965656No Hit
AGGACT3192891.3042873684668903No Hit
TCCACG3016501.2322325062812607No Hit
GGTACC3007811.2286826635895371No Hit
CGAGCC2972111.214099305235736No Hit
TTGAAT2708051.1062314731095535No Hit
GCTCAA2599651.0619503513854067No Hit
TCTTCA2547001.0404429615442967No Hit
GAATAC2460081.0049363646784035No Hit
TCATGT2354790.9619256699705122No Hit
CGTGAA2230810.9112801582421016No Hit
CCTTAT2228840.9104754182993289No Hit
CTTAGC2126170.8685349868655821No Hit
CCAGCG2090900.8541272824079191No Hit
CCACTT2070640.8458511244177788No Hit
TGTGAT1993330.8142701878818582No Hit
GGCTAC1961980.8014637933610834No Hit
CGTACT1899470.7759286188317807No Hit
GTGGTG1881610.7686328546805513No Hit
GTCTCA1839150.7512880536804842No Hit
ACTTGA1702140.6953198204016526No Hit
CTGTAT1698570.6938614845662726No Hit
GCGCAG1696390.6929709601625951No Hit
ATGCCG1685130.6883712790683709No Hit
TGATAA1680820.68661065513266No Hit
ACTAAT1676820.6849766654011417No Hit
ATGGCC1655450.6762470752605049No Hit
GGCCAG1648660.6734733776912525No Hit
CAGATC1642000.6707527847882745No Hit
AACATA1628570.6652666642647018No Hit
GATCAG1627470.6648173170885343No Hit
CAGTAG1525140.6230157747819666No Hit
CCGGAA1500730.6130443524453759No Hit
TAGCTT1463110.5976766790204461No Hit
GCCATA1423450.5814756708324418No Hit
ATTGAA1369380.5593882146366427No Hit
GTACTT1366420.5581790622353192No Hit
GTCTGC1281180.5233587410566636No Hit
GGCCGT1272600.5198538330825567No Hit
TTCTAC1256420.5132443446185652No Hit
GCTGTC1238140.5057770115455263No Hit
CTGAGG1227800.5015531480895514No Hit
CATGAG1225350.5005523293789965No Hit
AAGTTG1221950.4991634381072059No Hit
TCCAGA1190110.48615687984432No Hit
CTATTA1166170.47637745130118275No Hit
TTAGTA1159180.4735220542453545No Hit
GACTTG1157780.47295015783932304No Hit
AATGTA1148280.46906943222696706No Hit
GCACAT1133640.4630890298096099No Hit
CAGTCC1133090.4628643562215262No Hit
TGTGTG1122320.4584648388694131No Hit
TTCGTT1103500.45077691718261936No Hit
AGTTAG1102350.45030714513480785No Hit
TACGGC1063670.43450646443102553No Hit
CCATGA1051520.4295432206215387No Hit
CAAGGA1049640.42877524544772505No Hit
AAGCAT1048520.42831772832289994No Hit
ACGCTA1041970.42564207013753863No Hit
GTCGAG1036460.42339124928237215No Hit
ATTCAT1017560.41567064780094803No Hit
CATTAA1005720.4108340381956538No Hit
GAGCCA985390.4025292853852118No Hit
AAGCGC985220.4024598408216223No Hit
CCGTCG964850.39413874811386523No Hit
GGTAAT962670.39324822371018775No Hit
TCGTGG958360.39148759977447667No Hit
ACAGCC946930.3868184741166631No Hit
GAGATA937810.38309297752880134No Hit
AGAACG935080.38197777953704004No Hit
TACAGT932250.3808217318019908No Hit
TAGAGC913820.3732931241140201No Hit
ACCAGG909210.37140995094844526No Hit
CGAAGC902980.3688650119416055No Hit
GACACG900640.36790912794866726No Hit
TAAGTC886180.3620022550692285No Hit
TTCACA878140.3587179357088766No Hit
GTTGGC872970.35660600398088915No Hit
CTCGTC857830.3504213528470923No Hit
CCTGCT851690.34791317860921167No Hit
CTACCG850540.34744340656140016No Hit
GATCCT847340.3461362147761855No Hit
CACATT831710.3397513999002776No Hit
TATTGG819340.3346982866555572No Hit
AACTAG812810.3320307984188535No Hit
GTAAGA802570.3278477847061666No Hit
TGCGAC801350.3273494178380535No Hit
TCAAGC792580.32376689535169956No Hit
CCTCGG782920.3198208101500828No Hit
CGTCAG782560.3196737510742461No Hit
GTACGC778310.3179376369845079No Hit
ATCAGC754530.30822356803063145No Hit
AAGAGG751610.30703075552662307No Hit
AGACGG745550.3045552610833728No Hit
CGAATT741840.30303973560738956No Hit
CGAGAG731110.2986565581525916No Hit
ATGTGA730140.2982603156426984No Hit
GGCGTT724720.2960462595564911No Hit
TATAAG721810.2948575320268115No Hit
GACCGA719210.2937954387013246No Hit
CTGGAG709150.28968595452655593No Hit
GGACTA706340.28853807674016435No Hit
GCCAAT704970.2879784352571193No Hit
GATTCA703640.2874351336713895No Hit
CGATGT701760.28666715849757585No Hit
TCTCTG686450.28041306280018946No Hit
TAATGA644340.2632112359016302No Hit
GACCAT634800.259314170391959No Hit
CTAGTT628940.2569203754352847No Hit
CAACGG619580.2530968394635318No Hit
GGATCG610990.24958784651509616No Hit
ATTCGA599500.24489421101130973No Hit
AGCCTA597170.2439424119927003No Hit
AACTCT594320.2427781943089935No Hit
GGAGGC587690.24006985632900185No Hit
AACAAT580730.23722671419616No Hit
GACTAA577400.23586641774467096No Hit
AATTGC570840.2331866745849809No Hit
TGACCA568230.2321204962851652No Hit
GAGTCG565480.23099712834474634No Hit
ACAGTG541760.22130756923684267No Hit
TTCTGT541710.22128714436519867No Hit
AGGTCA525750.21476752533644053No Hit
GGTGGT515930.21075608054556302No Hit
ATACCT506690.2069815642657557No Hit
GAACCG504560.2061114647337222No Hit
AGTGCA498910.2038034542379525No Hit
CACCTA485790.19844396791857238No Hit
ATTGCT484010.19771684248804677No Hit
TTAGGC474140.19368497282552527No Hit
CTATGC466330.19049460787473574No Hit
TGGCAT464720.1898369270077996No Hit
TTAGCG458970.18748806676874202No Hit
AGGCTT454560.18568659308974303No Hit
CCGCTC450880.18418332253674619No Hit
GCATAA443600.1812094612253828No Hit
CGCTTG422750.17269228974984352No Hit
AGTTCC416570.1701677756146477No Hit
CTTCAC412090.16833770711534718No Hit
TTCCAT410380.16763917650512308No Hit
TACGAA399100.16303132546224142No Hit
TCTCGC392630.1603883470715105No Hit
TCGGAC385200.15735321114521522No Hit
CAAGCT378260.1545182389610309No Hit
ACCTGC371930.15193245021090315No Hit
CTCCGC367440.15009829673727382No Hit
AGATGC366340.14964894956110628No Hit
GCGTAT361230.14756152767909161No Hit
GGCAGG360190.14713669034889684No Hit
TGCGGA346390.14149942577515862No Hit
TCCTCC345680.14120939259781412No Hit
AGTAGG344540.14074370552433138No Hit
GCCTGG344290.14064158116611147No Hit
GAGTGA341360.1394446836877743No Hit
CCTGAC336080.1372878172421701No Hit
TGACAC330520.13501657151535962No Hit
TACTCG317690.1297755494515146No Hit
CGACAA317230.12958764063238998No Hit
AGGTGT316740.12938747689027896No Hit
TATGGT312170.1275206436220193No Hit
GCGGCT310980.12703453167689258No Hit
GTTACA310720.1269283223443439No Hit
AGTCAA294880.1204577230075313No Hit
ACGTAA292410.11944873434831874No Hit
GGATTC281990.11519219109771349No Hit
CCGACT281260.1148939879717114No Hit
CATAAC280580.11461620971735327No Hit
CGTCTC273360.11166685825196268No Hit
ATCCAC251080.10256553544740558No Hit
AGAGGT250320.10225507739841712No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)