FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368486664
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGC42046171.1410499784057313No Hit
GTCTCA38447721.0433951552721592No Hit
AACATA34204590.928244990706095No Hit
CAGCCT33869980.9191643364330818No Hit
GTACTT33817170.9177311773757978No Hit
GGCCGT33795010.917129798759827No Hit
GGCTAC33727010.9152844131151514No Hit
CATACG31557820.8564168824302418No Hit
TACGGC30962240.8402540179853022No Hit
GCTGTC30534990.8286592971516602No Hit
CAGATC30204360.8196866522149089No Hit
GAATAC29719770.8065358370744186No Hit
GCCATA29552290.8019907607836793No Hit
CCTTAT29218220.7929247610437267No Hit
GAGAAG29013100.787358209522611No Hit
TCATGT28933210.7851901527703592No Hit
TAGCTT28535770.774404416437714No Hit
CGTTGC28482250.7729519893832576No Hit
AACCTT28437350.7717334920972879No Hit
CCACTT28420070.7712645470393469No Hit
CTGTAT28261700.7669666981489458No Hit
GAGATA27636470.7499991912868793No Hit
TGTGAT27128610.7362168743235711No Hit
TTACGT27037990.7337576265717991No Hit
CATGAG26373260.7157181677543695No Hit
GCACAT26352430.7151528827105667No Hit
GTGGTG25939830.7039557339312557No Hit
GAAGAT25818470.7006622633159935No Hit
CTGTTC25795850.7000484012088969No Hit
AAGCGC25439750.6903845507961178No Hit
ACTTGA25087390.6808221965937958No Hit
GCTCAA24787590.6726862169427114No Hit
CACATT24688690.6700022663506758No Hit
GAGCAC24632350.6684733100680138No Hit
ACAGCC24564890.6666425789563988No Hit
TTAGTA24480290.6643467021102288No Hit
GATCAG24369150.6613305821021517No Hit
CCTCGG24195360.6566142648787963No Hit
GACTTG23991690.651087063492751No Hit
CAGTCC23639110.641518738925108No Hit
ACTAAT23494740.6376008223733166No Hit
CAGGAC23321290.6328937320781846No Hit
CGAATT23311350.6326239801177717No Hit
TCGTGG23229820.630411417005854No Hit
GCTTCT23017240.6246424158243078No Hit
GTAAGA22846090.6199977429848045No Hit
CTGGAG22792070.6185317469182547No Hit
TCTTCA22601010.6133467560172001No Hit
TGCATA22460040.6095211087476425No Hit
TCCAGA22429220.6086847148422175No Hit
ACGAAC22158320.6013330240901201No Hit
AGTTAG22068100.5988846315480226No Hit
TGATAA21872120.5935661215679707No Hit
CCGTCG21812580.5919503236079122No Hit
GGTAAT21812180.5919394683982376No Hit
CAAGGA21787990.5912829995931684No Hit
AAGGCG21787640.5912735012847032No Hit
GTCGAG21735210.58985065467661No Hit
GGTACC21560260.5851028573451983No Hit
CCATGA21283360.5775883384479824No Hit
AACTAG21198210.57527753568851No Hit
CCAGCG21052620.5713265107472112No Hit
GGACTA21035700.5708673353779772No Hit
ACACGA20935440.5681464770730482No Hit
GGCCAG20905450.5673326077276979No Hit
TCAATG20838180.5655070328406784No Hit
ATGCCG20829790.5652793448177543No Hit
CGTACT20699980.561756557898117No Hit
TAGAGC20666160.5608387499201328No Hit
ACCAGG20286030.5505227727861544No Hit
ATGGCC20236370.5491750985050574No Hit
CGAGCC20157460.5470336370165082No Hit
GTTGGC20125590.5461687481856874No Hit
AGGACT20007170.5429550633615332No Hit
ACTAGC20004810.5428910176244534No Hit
CCTGCT19992320.5425520637023651No Hit
CAGTAG19960350.5416844610691257No Hit
TACAGT19552790.5306240879317141No Hit
GCGCAG19165130.5201037614756121No Hit
CGAAGC18885410.5125127133501906No Hit
GACACG18658280.5063488539167322No Hit
ATCCGG18494450.5019028314142734No Hit
TCCACG18367250.49845087473776256No Hit
CAACGG18027250.48922394651438456No Hit
TTGAAT17523160.4755439399022593No Hit
GTCTGC17196670.46668364638563964No Hit
CTGAGG16451430.44645930524096256No Hit
ATTGAA16018270.43470419868437904No Hit
AAGAGG15904160.4316074787444682No Hit
GAGTCG15751870.4274746290411205No Hit
CCGGAA14933860.40527545387639863No Hit
TTCTAC14177480.384748795142285No Hit
CGTGAA14049510.3812759421871506No Hit
CCGCTC12535070.34017703283829015No Hit
ATTCAT12491680.33899951396884204No Hit
GACTAA12296860.33371248409684645No Hit
AACTCT12163870.33010339826029633No Hit
CGATGT11896250.3228407202275304No Hit
GCATAA11876800.3223128856571048No Hit
AGGCTT11507790.31229868335207916No Hit
GCCAAT11116740.3016863589939852No Hit
AGTGCA11002890.2985966949403629No Hit
GGCGTT10475970.28429712723606193No Hit
GAACCG10317360.2799927652198561No Hit
CACCTA10047900.2726801532225872No Hit
AGACGG9716120.26367629955802147No Hit
GAGTGA9458870.2566950428360686No Hit
TTAGCG9394250.254941383713143No Hit
GACCAT9386530.25473187816642395No Hit
CTAGTT9342370.2535334630183523No Hit
GCGTAT9231380.2505214137139031No Hit
AGTAGG9136820.24795524214683656No Hit
TATGGT9098070.24690364370961332No Hit
AGAACG9052620.2456702205103412No Hit
ATCAGC8531370.23152452540317714No Hit
TGACCA8518450.23117390213068878No Hit
ACGTAA8440500.22905849314535845No Hit
TTCCAT8428660.2287371789389914No Hit
TGCGAC8382570.22748638740423993No Hit
TCTCGC8359270.2268540714406967No Hit
GTTACA8337070.22625160730375846No Hit
CTACCG8331890.2261110323384729No Hit
TATAAG8300880.22526948220845244No Hit
CTCCGC8294370.22509281367099893No Hit
AGTTCC8288210.2249256434420107No Hit
CTTCAC8108010.22003537148362037No Hit
GCGGCT8058740.21869828103195615No Hit
TCGGAC8006190.21727217786096054No Hit
CAAGCT7996400.21700649660417565No Hit
ACCTGC7944930.21560970249930128No Hit
AGATGC7937720.21541403734491732No Hit
TACGAA7928280.21515785439659763No Hit
ACAGTG7924340.2150509305813032No Hit
AGTCAA7773130.21094738994407677No Hit
TTCACA7724120.20961735537870105No Hit
TGCGGA7717580.20943987270052195No Hit
AGCCTA7358400.1996924371732487No Hit
CTATTA7329100.198897293064587No Hit
CCTGAC7322320.19871329726060316No Hit
TCCTCC7252040.19680603692078255No Hit
GGATTC7222720.19601035005163714No Hit
GATTCA7220540.19595118915891077No Hit
GGCAGG7220270.19594386189238044No Hit
TGTGTG7177510.19478343997816974No Hit
TTCGTT7167770.19451911562259414No Hit
AACAAT7080540.19215186577281396No Hit
TGACAC7028180.19073091882641377No Hit
ACGCTA6994820.18982559433955526No Hit
AGGTGT6987820.1896356281702504No Hit
TTCTGT6963210.18896776139502297No Hit
GGTGGT6915110.18766242243165684No Hit
ATCCAC6886820.18689468772742343No Hit
AAGTTG6866510.18634351445619754No Hit
TTAGGC6847360.18582382129302785No Hit
ATGTGA6703120.18190943268438067No Hit
ATACCT6699930.18182286238722603No Hit
AATGTA6597640.1790469138931986No Hit
AAGCAT6493670.17622537351853795No Hit
GAGCCA6445750.17492491939952542No Hit
CGACAA6289670.17068921658451117No Hit
AATCGG6207810.168467697924612No Hit
AATTGC5958410.16169947469252238No Hit
ATTCGA5956380.16164438450342397No Hit
TACTCG5909650.16037622463319323No Hit
AAAAAA5861880.15907984121780863No Hit
ATTGCT5697430.1546169931403542No Hit
TATTCT5674410.15399227582358313No Hit
CTCGTC5655220.153471497139446No Hit
CGTCTC5641000.1530855944355153No Hit
ACCGCT5598100.1519213731979185No Hit
GATCCT5579580.15141877698998626No Hit
CATAAC5488400.14894433194466977No Hit
CGCTTG5368470.14568966870399414No Hit
CATTAA5294980.14369529530653516No Hit
AGAGGT5112010.1387298510211485No Hit
AGGTCA5066720.13750076990574617No Hit
GTACGC5065210.13745979148922471No Hit
TAAGTC4962780.13468004367181116No Hit
TATTGG4912740.13332205694152338No Hit
CGAGAG4735600.12851482733714348No Hit
CGTCAG4642470.1259874631446635No Hit
GGGGGG4523330.12275423894309512No Hit
GCCTGG4324010.11734508796226069No Hit
GACCGA4169740.11315850497102387No Hit
TCAAGC4146580.1125299883308667No Hit
TCTCTG4116510.11171394794358148No Hit
GGAGGC3956770.10737891996004502No Hit
GGATCG3732320.10128779043140622No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)