FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22804526
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGAC7318003.2090121057547965No Hit
ATCAGC6763832.9660033275850592No Hit
AACATA4395491.9274638727417532No Hit
GAACCG3985511.7476837711952444No Hit
GTCTCA3941041.728183256253605No Hit
ACTTGA3769661.653031507868219No Hit
ATTGCT3411351.4959091892547995No Hit
TACTCG3354521.470988697594504No Hit
GATCAG3325581.458298234306646No Hit
GCGCAG3268551.4332900407576987No Hit
TCATGT3254861.4272868464795103No Hit
TCTTCA3244091.4225640997756324No Hit
GACTAA3230451.4165828309696065No Hit
GGCTAC3223701.4136228922276217No Hit
ACTAAT3100071.3594099697577577No Hit
CAGATC2996441.313967236153034No Hit
TTCCAT2901201.2722035967772363No Hit
GCGTAT2881781.263687743389185No Hit
CGATGT2856931.2527907837242485No Hit
TTCACA2841601.2460684339591184No Hit
CTACCG2801301.228396503395861No Hit
CTTCAC2796271.2261908008962783No Hit
AGTGCA2791351.2240333344354537No Hit
TTAGCG2759241.2099527962124712No Hit
GATTCA2675631.1732890216617524No Hit
TGACCA2668601.1702063002756558No Hit
ACAGTG2573101.1283286484446113No Hit
TAGAGC2500231.0963744653144731No Hit
AAGCGC2495031.0940942162095366No Hit
TTCTGT2491111.0923752591919693No Hit
AACTCT2486501.0903537306585542No Hit
ATTCAT2460831.079097193250147No Hit
CTCCGC2436181.0682879354738617No Hit
AATTGC2428721.0650166550271642No Hit
TATAAG2425821.063744977641719No Hit
GCCAAT2402011.0533040677977696No Hit
TACGAA2363351.0363512927214535No Hit
AGTTCC2361721.0356365223289448No Hit
TGCGAC2358381.0341719007884662No Hit
TTAGGC2350881.0308830799640387No Hit
CCTGAC2331851.0225382452588578No Hit
ATGTGA2322421.0184031012089443No Hit
TCCAGA2303091.0099267136707861No Hit
ACCAGG2255260.9889528069998035No Hit
CCGCTC2220460.9736926783744595No Hit
CTGAGG2217000.9721754357007903No Hit
CGTACT2205270.9670317199313855No Hit
GCTGTC2203950.9664528874662863No Hit
TGTGAT2201840.965527632541014No Hit
GCTCAA2169570.9513769328071103No Hit
GACACG2134480.9359896364432219No Hit
GCCATA2132220.934998605101461No Hit
TGCGGA2124950.9318106414489826No Hit
GTACTT2114810.9273641556943565No Hit
CGTCTC2095890.9190675570279339No Hit
CCACTT2093350.917953743042061No Hit
CGCTTG2087660.9154586243099286No Hit
AGGTGT2046370.8973525693978468No Hit
GGTAAT2020410.8859688642508948No Hit
CATGAG1994890.8747781032589759No Hit
TGACAC1977690.8672357408349552No Hit
TAGCTT1965300.8618026088330009No Hit
TCCTCC1888370.8280680773632393No Hit
CAAGGA1863420.8171272667539768No Hit
TTCTAC1838350.8061338350115237No Hit
TCTCGC1836890.8054936112243684No Hit
CTGTAT1813550.7952588008187498No Hit
TGATAA1811770.7944782540097524No Hit
AGAACG1800650.7896020290007343No Hit
CTAGTT1799580.7891328238964493No Hit
CCAGCG1790350.7850853817351872No Hit
GTCTGC1768770.7756223479497009No Hit
TACAGT1749500.7671722709781382No Hit
CCGGAA1746300.7657690407597159No Hit
AGAGGT1679860.7366344733497202No Hit
CTTAGC1640290.7192826546800403No Hit
GTTGGC1616510.7088549001193886No Hit
CGACAA1600830.7019790720491187No Hit
CAGTCC1582940.6941341381092507No Hit
ATTGAA1570270.6885782234631844No Hit
GTGGTG1533800.6725857840676013No Hit
GGCAGG1532870.6721779702853723No Hit
ATTCGA1516900.6651749744765578No Hit
GACTTG1516180.6648592476774128No Hit
AGGTCA1514590.6641620176626342No Hit
GCGGCT1485680.6514847096580739No Hit
AGTTAG1477650.6479634788287203No Hit
GGCCGT1423650.6242839688928417No Hit
GCCTGG1409250.6179694329099408No Hit
GACCAT1368950.6002975023466832No Hit
ATACCT1303170.5714523511692372No Hit
GTCGAG1166080.5113370915931338No Hit
CGAAGC1159630.508508705684126No Hit
CCGTCG1067790.46823599841540225No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)