FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25028747
Sequences flagged as poor quality0
Sequence length6
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCC8270763.3045042166913112No Hit
AACTAG7922283.165272316668509No Hit
GAGTCG7846983.135186911274464No Hit
TACGGC6964492.782596348111234No Hit
CCTCGG6681452.6695103834003358No Hit
CAACGG6256452.499705638480424No Hit
TTACGT5527962.2086443240646445No Hit
GAGAAG4449281.7776678952406206No Hit
GAATAC4227611.6891017356961575No Hit
CAGCCT4000941.5985378732702842No Hit
GGTACC3792391.5152136860866428No Hit
CGTGAA3772521.5072748148359165No Hit
GAAGAT3676571.4689388965416448No Hit
CAGTAG3648461.4577078109423536No Hit
CCTTAT3204741.2804236664344404No Hit
GCATAA3075511.2287910377614988No Hit
CAGGAC3050141.2186546933412208No Hit
ATGCCG3036591.2132409185325976No Hit
ACACGA2991531.1952376201653243No Hit
GTTACA2979151.1902913078309512No Hit
ATGGCC2936261.1731550125142103No Hit
CGAGCC2864751.144583865904274No Hit
ACCAGG2863161.1439485963879854No Hit
GGCCAG2841721.1353824464324962No Hit
AAGGCG2836871.1334446746375277No Hit
GCTGTC2810331.1228408677429997No Hit
AACATA2796831.1174470699631907No Hit
TTGAAT2650281.0588943985090424No Hit
GTCTCA2621851.047535459925341No Hit
TCCACG2528971.0104261312002554No Hit
TATGGT2485020.9928663228726552No Hit
CTATTA2320870.9272817372759411No Hit
GACACG2213850.884522904802226No Hit
GTTGGC2192100.8758328972680893No Hit
CAAGGA2182840.8721331515317167No Hit
CATGAG2130850.851361036970808No Hit
TAGAGC1901250.7596265206564276No Hit
ACGTAA1879120.7507846877033038No Hit
GAGTGA1848930.7387225577053458No Hit
TTCTAC1825480.7293533311915295No Hit
TACAGT1789600.7150178153145261No Hit
CTGAGG1784090.7128163467392116No Hit
GTACTT1651460.6598252801069107No Hit
TTAGTA1645650.6575039493587115No Hit
CCTGCT1638770.6547551101938902No Hit
TCAATG1594340.6370035223896745No Hit
TGTGAT1572900.6284373724341854No Hit
AACTCT1477470.5903092152395804No Hit
GGCTAC1468350.5866654051838871No Hit
CCACTT1445890.5776917238405902No Hit
ACTAGC1435270.5734486029204738No Hit
TCATGT1428890.5708995340437937No Hit
GGCGTT1422430.5683185019210111No Hit
GTGGTG1382380.5523169018409112No Hit
CCGACT1301040.5198182713661215No Hit
AGCCTA1288570.5148360003798832No Hit
GTACGC1287680.514480409266992No Hit
TCGTGG1281190.5118873909269209No Hit
GCACAT1257130.5022744446615726No Hit
TCTCTG1215640.4856975061516264No Hit
TAGCTT1191130.47590476662695097No Hit
CCATGA1185650.4737152842689249No Hit
AGACGG1183110.47270045120516824No Hit
GATCAG1176080.4698916809539047No Hit
GAGCAC1163620.46491340537342923No Hit
AGGACT1157060.46229241919301833No Hit
GGTAAT1149650.4593318235227677No Hit
CTTAGC1128690.4509574530438939No Hit
GGTGGT1128380.45083359546524643No Hit
ACGCTA1101020.4399021652981669No Hit
AACAAT1088330.43483199538514655No Hit
AAGAGG1074160.4291705054192285No Hit
CGTTGC1064380.42526299858318917No Hit
GCTTCT978900.3911102701225915No Hit
CCTGAC966130.3860081369634684No Hit
CAGATC960900.3839185397495128No Hit
CGTACT960370.3837067832440833No Hit
GACTAA952280.38047449998196076No Hit
ACTTGA951830.38029470672263377No Hit
CTCGTC944690.37744198700797926No Hit
CGTCAG919150.36723772068973326No Hit
GGAGGC902850.36072520929633434No Hit
TATTGG887750.35469214659447396No Hit
GATCCT880210.3516796106493066No Hit
CGAGAG870680.34787198895733773No Hit
GAGCCA860950.34398445915011244No Hit
GGATCG833060.3328412724776035No Hit
GGCCGT822970.3288099080629166No Hit
TCCAGA809920.3235959035424346No Hit
GTCGAG799320.3193607734338439No Hit
AAGCGC790040.3156530368859456No Hit
GCGTAT788230.3149298684428749No Hit
CTTCAC787280.31455030489540686No Hit
TCCTCC751510.3002587384817945No Hit
AGTTCC737090.29449736337180604No Hit
TTAGCG728300.29098540170628595No Hit
GTAAGA690890.2760385887475709No Hit
CCGCTC669350.2674324847344535No Hit
GGCAGG650150.2597613056698364No Hit
CTGTAT645130.2577556119769No Hit
CGAATT626760.250416051590597No Hit
TGCGGA625710.24999653398550076No Hit
TCTTCA604490.24151828295679365No Hit
CCGGAA600620.23997206092658177No Hit
GCTCAA599500.23952457548114575No Hit
AACCTT598510.2391290303106264No Hit
GCGGCT587240.23462620801592665No Hit
TGATAA582060.23255658783078512No Hit
AATTGC565760.22604407643738617No Hit
GCGCAG557230.22263599532169948No Hit
CCGTCG553270.22105381463962218No Hit
TGACAC545220.2178375130005509No Hit
ATTCAT539590.21558809955608246No Hit
CGCTTG534690.21363035073229997No Hit
ATTGCT529780.21166860650275462No Hit
GATTCA519350.20750139829213182No Hit
GCCATA507840.20290268625912433No Hit
GGACTA503390.2011247306946688No Hit
ATTGAA499050.1993907245936043No Hit
CAGTCC498690.19924688998614273No Hit
TGTGTG483400.19313791457478874No Hit
AATGTA482730.1928702223886797No Hit
AGATGC472740.18887881203162107No Hit
CTGTTC472470.18877093607602488No Hit
GAGATA469650.18764423165090924No Hit
ATTCGA467050.1866054261525757No Hit
CGATGT466020.18619389935900507No Hit
GTCTGC460730.18408032971047253No Hit
CTGGAG459760.18369277535147885No Hit
CATTAA456420.18235830982669649No Hit
ATCCGG446950.17857466056930454No Hit
TGACCA435870.1741477509840984No Hit
CAAGCT432580.17283326248813013No Hit
CACATT429180.17147482452877086No Hit
GACCGA424520.16961296544329607No Hit
CTCCGC421730.16849824723546888No Hit
AAGTTG415880.16616093486421835No Hit
TACTCG412630.16486242799130135No Hit
ACCTGC408150.16307248620955736No Hit
CCAGCG404390.1615702136427365No Hit
ACAGTG402010.160619307071185No Hit
AGGCTT397640.15887331475283203No Hit
GACTTG388270.1551296195530683No Hit
CACCTA388190.15509765630696573No Hit
GCCAAT384090.15345953994420897No Hit
CGAAGC383100.15306399477368962No Hit
TTCGTT382830.15295611881809343No Hit
AGTTAG382600.15286422448554854No Hit
CATAAC370220.1479179121511756No Hit
CATACG363840.1453688432744955No Hit
GCCTGG351910.14060232419944954No Hit
TTAGGC347780.13895222161940426No Hit
TGCATA339900.13580384187830097No Hit
ACTAAT307560.12288269964133643No Hit
ACGAAC307440.12283475477218256No Hit
TCTCGC307290.12277482368574025No Hit
CGTCTC292640.11692155424320681No Hit
AGAGGT290720.11615443633674509No Hit
AGGTGT254380.10163513179465196No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)