FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001836460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374306316
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACT42131771.1255960212009888No Hit
CTGTAT38985041.041527709620588No Hit
AACCTT37203440.9939303295111911No Hit
TTACGT36476920.9745205581836882No Hit
GGCTAC36339530.97085003502853No Hit
TAGCTT33372880.8915927563455809No Hit
CCGTCG32698550.8735772975842598No Hit
CCTGCT31419690.8394111629150282No Hit
GTAAGA31030860.8290231469137165No Hit
CGAATT30494570.8146955767639251No Hit
GTCTCA29818820.7966421811594545No Hit
GGTAAT29729400.7942532286844981No Hit
GCTGTC29544280.7893075467099518No Hit
CTGAGG29145820.7786622547934777No Hit
TTAGTA28625900.7647720269833758No Hit
CTGTTC28512680.7617472316443626No Hit
TGATAA28057970.7495991598496029No Hit
ACCAGG27502450.7347578393520883No Hit
GCCATA27420080.7325572352885438No Hit
CAGCCT27329770.7301445054964021No Hit
GAATAC27218900.7271824929611928No Hit
GAGAAG27188840.726379407394237No Hit
TGTGAT26700380.7133296676725059No Hit
CAGTCC26686090.7129478947932046No Hit
GGTACC26189740.6996873651472127No Hit
TCAATG25867410.6910759689131187No Hit
TCGTGG25657450.6854666593443216No Hit
AACATA25559430.6828479485235296No Hit
GCGCAG25513480.6816203443385123No Hit
CTTAGC25461760.6802385883330913No Hit
GATCAG25434940.6795220628871248No Hit
CAGTAG25392520.678388766488247No Hit
CAGATC25261430.6748865546794567No Hit
GGCCGT25214300.6736274255121039No Hit
TTCTAC25193060.6730599758300632No Hit
ATTCAT24939810.6662941268669376No Hit
CAAGGA24708500.66011442884656No Hit
GAAGAT24705820.6600428297341367No Hit
CCGGAA24660790.6588398043489067No Hit
ATCCGG24543930.6557177624542141No Hit
GAGTCG24450220.6532141979672071No Hit
GCACAT24291510.6489740878430702No Hit
CCACTT24195680.6464138852522061No Hit
CGTGAA23851310.6372136664666914No Hit
ACAGCC23749730.6344998463771581No Hit
ACTAGC23660230.6321087566152638No Hit
GCTCAA23501610.627871050938932No Hit
GTGGTG23483080.6273760018519164No Hit
GGACTA23455290.6266335618018265No Hit
AACTAG23310820.6227738887526546No Hit
AAGCGC23304750.6226117221062334No Hit
TAGAGC23250780.621169854905681No Hit
TACGGC23057710.6160117800416705No Hit
CTGGAG23041650.6155827196888657No Hit
GTCTGC22994030.6143104996390176No Hit
CCTCGG22875120.6111336897665387No Hit
CCTTAT22139930.5914922899671294No Hit
GTACTT22041250.5888559465290989No Hit
GTTGGC21790760.5821638339653344No Hit
ATTGAA21719320.5802552367296949No Hit
GAGATA21713960.5801120385048485No Hit
TCATGT21696630.5796490487219029No Hit
TCTTCA21471650.5736384635304952No Hit
CGAAGC21253300.5678050059940747No Hit
AGGACT21201070.5664096247844239No Hit
CATACG21091550.5634836789662935No Hit
CAGGAC20842370.5568265644761389No Hit
GACTTG20789610.5554170237405237No Hit
ACTTGA20507430.5478782783884416No Hit
TACAGT20391350.5447770750413947No Hit
ATGCCG20253720.5411001400254224No Hit
CAACGG20161760.5386433286901843No Hit
AGTTAG20010090.5345912998165919No Hit
CCATGA19959240.5332327868066218No Hit
GACACG19831130.5298101889362722No Hit
CGTTGC19817050.5294340264351831No Hit
CATGAG19614450.5240213472646826No Hit
GAGCAC19565590.5227159992672953No Hit
CACATT19497950.5209089231612111No Hit
GGCCAG19441600.519403471674253No Hit
ATGGCC19300700.5156391750546897No Hit
TGCATA19245120.5141542949545099No Hit
AAGAGG19018730.5081060400808198No Hit
GTCGAG19008150.5078233838832684No Hit
ACACGA18947120.506192901110437No Hit
CGAGCC18735310.5005341667811986No Hit
AAGGCG18455770.4930659518980706No Hit
ACGAAC17619380.4707208841220836No Hit
TCCAGA17524720.4681919393526878No Hit
GCTTCT17260780.461140495422471No Hit
TTGAAT17038450.45520070786088473No Hit
AACTCT16761420.44779955035543667No Hit
CCAGCG16699240.4461383440828714No Hit
ACTAAT15530110.41490376560998243No Hit
TCCACG14883230.39762166342926475No Hit
CTATTA14718190.39321244047615805No Hit
CCTGAC12435730.3322340411696393No Hit
CGATGT11706290.31274625886889923No Hit
GGCGTT11615750.3103273843768108No Hit
CTCCGC10674630.2851843408381065No Hit
TTCCAT10180740.2719895327654583No Hit
TGACCA9827620.2625555482210992No Hit
AAGCAT9806360.26198756421732405No Hit
GCATAA9687650.2588160975621902No Hit
GTTACA9508800.25403792545141024No Hit
GCGTAT9455410.2526115535811584No Hit
GACTAA9322730.24906686319447519No Hit
AGCCTA9308550.24868802908471363No Hit
CGCTTG9268440.24761644684617076No Hit
AGATGC9220410.2463332732007653No Hit
TTCTGT9176410.24515776538486198No Hit
CCGACT9159680.24471080525395145No Hit
CTTCAC9135660.24406908485081505No Hit
AGACGG9055340.24192324876505691No Hit
CTAGTT9013550.2408067835008159No Hit
GTACGC8995860.24033417592664932No Hit
ACAGTG8910700.23805903398114178No Hit
TACTCG8903950.23787870039574752No Hit
GGTGGT8893040.23758722788957695No Hit
GCCAAT8881450.2372775884444333No Hit
AGTTCC8855320.23657949709830706No Hit
TGTGTG8830130.23590651887370234No Hit
TTAGCG8703200.23251544598568838No Hit
AGGCTT8676820.23181067561788082No Hit
AATCGG8638920.23079813593100043No Hit
ATTGCT8616530.23019996274922594No Hit
TGCGAC8596750.22967151855380394No Hit
ACCGCT8465810.2261733141580224No Hit
AGTCAA8444210.22559624668476072No Hit
CCGCTC8414650.22480651916116745No Hit
TATGGT8408980.22465503894943625No Hit
AATTGC8287560.22141117169927746No Hit
CAAGCT8144530.2175899698149897No Hit
TCGGAC8116810.2168493998909706No Hit
TACGAA8094230.21624615065271835No Hit
AATGTA8006730.21390849306427412No Hit
GGCAGG7991310.21349653100697347No Hit
AGTGCA7965300.2128016455912542No Hit
TGGCAT7961230.21269291111828312No Hit
TTAGGC7952440.2124580767159697No Hit
TCTCTG7776010.2077445575350644No Hit
ACGCTA7775850.20774028296118838No Hit
ATGTGA7764510.20743732253772604No Hit
AACAAT7715660.20613224170120603No Hit
ACCTGC7684970.2053123249996134No Hit
TCTCGC7424630.19835705898160694No Hit
TCCTCC7254180.19380330199931758No Hit
GACCGA7245770.19357861970995968No Hit
GCGGCT7114680.19007640790116936No Hit
TAAGTC7108640.1899150427373499No Hit
AAAAAA7094920.18954849802748186No Hit
CACCTA7094360.18953353701891582No Hit
GATTCA7077190.18907482180984625No Hit
AGAACG7019320.18752876187106604No Hit
CGTCTC6966100.18610693173555748No Hit
TGACAC6952370.18574011986482217No Hit
AGAGGT6932510.18520953838246215No Hit
ATACCT6913440.1847000626086149No Hit
TTCGTT6874100.1836490517568504No Hit
CATTAA6855670.18315667427850724No Hit
GGAGGC6645650.1775457617445066No Hit
AGGTCA6637240.1773210794551487No Hit
CATAAC6635840.17728367693373362No Hit
AAGTTG6612090.17664916987401302No Hit
GAACCG6593620.17615572375220084No Hit
TGCGGA6584150.17590272241091437No Hit
CTCGTC6543500.17481671348554No Hit
TATAAG6532980.1745356602531922No Hit
ATCCAC6473740.17295299927559865No Hit
AGGTGT6425990.17167730613447624No Hit
CTACCG6414010.1713572474155098No Hit
ATTCGA6404130.17109329247866606No Hit
ACGTAA6350160.16965142527811367No Hit
TCAAGC6275310.16765172618674165No Hit
TAATGA6208730.16587296913258606No Hit
GCCTGG6056350.16180197183741885No Hit
GATCCT6000900.16032056482851334No Hit
GAGTGA5992900.16010683613471274No Hit
CGAGAG5974760.15962220632151983No Hit
CTATGC5879970.1570897884608498No Hit
CGTCAG5872710.15689582967122576No Hit
GAGCCA5811670.15526507973752707No Hit
GGATTC5765840.1540406814829168No Hit
GACCAT5562430.14860636228216892No Hit
TATTGG5548740.1482406190549026No Hit
ATCAGC5406170.1444317065705084No Hit
GGATCG5357820.14313998377735096No Hit
TTCACA5111090.13654832369967276No Hit
TATTCT4901620.13095210501337093No Hit
CGACAA4654730.1243561703618167No Hit
AAAACA3748520.10014578541068488No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)