FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27127752
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAC12954544.775382788813463No Hit
GCTTCT10603233.9086283301321836No Hit
AGGACT9667633.563741662044094No Hit
TCAATG9293623.4258717788337196No Hit
CGTTGC9245503.4081334863279493No Hit
ACTAGC5583902.0583718105355726No Hit
AACATA5391531.9874591893939462No Hit
TGTGTG4937611.820132386937185No Hit
GTGGTG4893501.8038722854735623No Hit
ACCAGG4426601.631760715005062No Hit
CATTAA4203801.5496307987480866No Hit
GACACG4131381.522934889702619No Hit
GACCGA3985591.469192876726387No Hit
GTAAGA3762831.3870777055172134No Hit
GACTAA3703121.365067035410822No Hit
AAGTTG3560751.3125857240216587No Hit
TTCGTT3539591.3047855937344164No Hit
CTGTTC3491011.2868777331789232No Hit
CGAATT3478741.2823546897656686No Hit
GGACTA3475301.2810866156546994No Hit
CATACG3282781.2101187005838154No Hit
AATGTA3272351.2062739293694515No Hit
GTTGGC3250131.1980830553154571No Hit
CACATT3238671.1938585991201924No Hit
GAGATA3167771.1677230018912No Hit
CATGAG3126951.152675680609289No Hit
AACCTT2904961.0708443515703034No Hit
ATCCGG2763141.018565784588417No Hit
CTGGAG2729231.0060656703142965No Hit
TTCTAC2579210.9507643685330063No Hit
ACGAAC2570290.947476222873167No Hit
TGCATA2293930.845602687609353No Hit
TGTGAT2188600.8067752904848142No Hit
GAAGAT2175120.8018062093755501No Hit
GGTACC2158850.7958086611820988No Hit
TCATGT2158670.7957423084669898No Hit
CCACTT2110390.7779450357700115No Hit
CTGAGG1981480.7304254329662111No Hit
CAGGAC1955120.7207084464647127No Hit
CGAGCC1728420.6371408880470449No Hit
ACACGA1685490.6213157654935801No Hit
GAGAAG1565850.5772133275178865No Hit
TTACGT1546710.5701578221446436No Hit
GGCTAC1514600.5583212350216118No Hit
ACTTGA1434860.5289269822283836No Hit
GTCTCA1393420.5136511127055422No Hit
TCGTGG1379090.5083686993304863No Hit
CTTAGC1368580.5044944380205186No Hit
CGTACT1366630.5037756169401725No Hit
AATCGG1335650.4923555774175465No Hit
TTGAAT1332770.4912939339758045No Hit
TTAGTA1317390.48562446309594687No Hit
TCCACG1297470.4782814292905656No Hit
CAGCCT1261800.4651325329131584No Hit
AACTCT1229820.45334386719548303No Hit
CAGATC1229130.45308951512089907No Hit
TAGCTT1224890.45152654005389015No Hit
CCATGA1217200.4486918046139614No Hit
GATCAG1213870.4474642793844474No Hit
CAAGGA1206360.44469589665962733No Hit
CGTGAA1197980.4416068091451146No Hit
TAGAGC1193950.440121245579066No Hit
GCACAT1191980.439395051974819No Hit
ACAGCC1186890.4375187446420183No Hit
CTATTA1154690.42564898116143207No Hit
ACCGCT1149270.42365102718426506No Hit
CCGCTC1069140.39411301017496764No Hit
GGTAAT1050800.38735240575776425No Hit
GTACTT1030200.37975870613974944No Hit
TACAGT1014040.3738017068277534No Hit
GCGTAT1009460.37211339885442773No Hit
AAGCAT999700.36851560719074694No Hit
NNNNNN978550.36071916316545505No Hit
TCTTCA966590.3563103938726659No Hit
GCTCAA959930.35385534341363784No Hit
TGGCAT958670.35339087440787575No Hit
TATTCT949670.3500732386524324No Hit
CCTGCT947620.3493175549525814No Hit
AAGAGG935530.3448608642544358No Hit
AACTAG912830.3364930496268176No Hit
GCGCAG911980.3361797173610257No Hit
TCCTCC886290.3267097104102102No Hit
TGCGGA881710.32502140243688454No Hit
AATTGC877230.3233699570830639No Hit
CCAGCG854120.3148510057154754No Hit
TCAAGC844380.3112605865756956No Hit
TAAGTC843300.3108624702850424No Hit
TCCAGA832360.3068296997112035No Hit
GGATTC829460.30576068374556065No Hit
CCTCGG819320.30202281412776116No Hit
ATCCAC814260.30015756558081186No Hit
AGTCAA802400.2957856589075276No Hit
GAGTCG782940.288612193151869No Hit
TAATGA755190.27838281623925193No Hit
GTCGAG742870.27384134151624506No Hit
GCGGCT739640.27265067890623595No Hit
GGCCGT738320.2721640923287709No Hit
TGATAA733390.2703467651871781No Hit
ACTAAT720660.265654153724201No Hit
CAACGG716060.26395847322697435No Hit
GATTCA710450.26189048027274797No Hit
CTATGC709160.26141495248113444No Hit
CCGGAA704770.25979668348486823No Hit
ATTGCT696170.25662649820744454No Hit
ATTCGA682130.2514509864289529No Hit
AAGCGC672660.2479600963618364No Hit
TCTCTG666890.24583312321640216No Hit
ATTCAT657480.2423643507209886No Hit
GATCCT645840.23807354181061519No Hit
CTGTAT626660.23100329138957035No Hit
CGAGAG607850.22406943266069373No Hit
GTCTGC606460.2235570422495753No Hit
AAGGCG581400.21431926980164076No Hit
TGACCA571050.21050398868288092No Hit
ATTGAA561070.20682509925628928No Hit
CGTCAG550100.20278126989659886No Hit
CCGTCG544540.200731708252125No Hit
GGATCG530720.1956372942365442No Hit
TATTGG491640.18123138253401905No Hit
CATAAC490780.18091436400627667No Hit
CGAAGC475980.17545869631954758No Hit
GAGCCA459090.16923259988516556No Hit
GCCAAT443370.16343779609899117No Hit
CGATGT440660.1624388191104077No Hit
CGCTTG435880.16067678589807222No Hit
GCCTGG435350.1604814140146961No Hit
CAGTCC434170.16004643510453798No Hit
AGTTAG431710.1591396146647168No Hit
CAGTAG425870.15698683768562907No Hit
ACAGTG421970.15554919552493698No Hit
GTACGC421410.1553427648557094No Hit
CACCTA418810.15438433674858132No Hit
GAATAC417540.15391618148086875No Hit
CCTTAT412360.15200669779051357No Hit
GGAGGC411240.1515938364520584No Hit
TACGGC409070.15079391760880148No Hit
GCATAA400590.14766796747478375No Hit
AGATGC399870.14740255661434828No Hit
ACGCTA399550.14728459623193252No Hit
CAAGCT390750.14404068571549902No Hit
TTAGGC362580.13365648580096132No Hit
AGTTCC358730.13223727495002166No Hit
GCCATA351950.12973798934758765No Hit
CTCGTC340070.12535871015040245No Hit
TGCGAC339650.12520388714848174No Hit
GACTTG337750.12450349737788814No Hit
ACCTGC337220.12430812549451203No Hit
CTTCAC329750.12155448781749406No Hit
TTCACA329410.12142915491117731No Hit
ATGTGA325710.12006523798949503No Hit
TATGGT325310.11991778751147533No Hit
AGGCTT317590.11707199328569504No Hit
AGTAGG307760.1134483977883608No Hit
CTCCGC304350.11219138246324281No Hit
GTTACA300520.11077954413620412No Hit
GGCCAG298420.11000542912660068No Hit
ATGCCG292700.10789688729091892No Hit
CCTGAC283230.1044059972238024No Hit
TGACAC279430.10300521768261521No Hit
AGAACG273980.10099620491959672No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)