FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001836466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29950701
Sequences flagged as poor quality0
Sequence length6
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGC6110612.0402226979595572No Hit
ATTCGA5886581.9654231131351483No Hit
ATTGCT5834661.9480879596106948No Hit
TCTTCA5655511.8882729990192884No Hit
TTCTAC5439351.816101065547681No Hit
TGCGAC5223861.7441528330171638No Hit
TAGAGC5181061.729862683347545No Hit
TTCCAT5091521.6999668889218986No Hit
TACAGT5055861.6880606567438938No Hit
TTCACA4993001.6670728341216454No Hit
GATTCA4975381.6611898332529846No Hit
CCACTT4807281.6050642687795522No Hit
TGATAA4792981.6002897561562917No Hit
AGTGCA4717481.575081665033483No Hit
TGTGAT4703561.5704340275708404No Hit
AGAACG4572001.5265085114368442No Hit
GCCATA4529121.5121916512070952No Hit
CTTCAC4507211.5048762965514562No Hit
TACGAA4417181.4748168999450129No Hit
GGCAGG4347371.4515085974114594No Hit
CCAGCG4335751.4476288885525583No Hit
CTGAGG4333231.4467875059084594No Hit
ATGTGA4331521.4462165676856777No Hit
CGTACT4253211.4200702681382984No Hit
CAAGGA4177361.3947453183149203No Hit
CAGTCC4143491.383436734919827No Hit
CCGGAA4102671.369807671613429No Hit
CTGTAT4057131.3546026852593533No Hit
TTCTGT3979341.3286300043528196No Hit
GTACTT3922091.3095152597596964No Hit
CCTGAC3908001.3048108623567776No Hit
AGTTAG3891351.2992517270296944No Hit
TCGGAC3698111.234732369035369No Hit
CGACAA3685141.2304019194742721No Hit
GACTTG3649301.2184355885359746No Hit
TGACAC3636721.2142353529555119No Hit
GACTAA3614161.2067029749988154No Hit
CTAGTT3437031.1475624560506947No Hit
ATACCT3404381.136661208697586No Hit
AACTCT3366081.1238735280352872No Hit
ACCAGG3364751.1234294649731236No Hit
GAACCG3333501.1129956524222921No Hit
GACCAT3313421.1062913018296299No Hit
CTACCG3302811.1027488137923716No Hit
CTATTA3272091.0924919587024022No Hit
GTGGTG3247981.0844420636431849No Hit
ATTCAT3238361.081230118787537No Hit
ATTGAA3038671.0145572218827197No Hit
GCGCAG3030031.011672481388666No Hit
GTCTGC3021831.008934648975328No Hit
AACATA3018261.0077426902295208No Hit
AGTTCC2986110.9970083838772255No Hit
AGGTCA2982560.9958231027714509No Hit
CCGTCG2959810.9882272872344456No Hit
GCGTAT2904660.9698136948447384No Hit
TGCGGA2886290.9636802824748577No Hit
TCATGT2844050.9495771067261497No Hit
GTCTCA2809680.9381015823302432No Hit
CCGCTC2776120.9268965023556545No Hit
GATCCT2763530.9226929279551753No Hit
TCCTCC2682700.8957052457636968No Hit
GACACG2676080.8934949469129286No Hit
ATCAGC2659180.887852341085439No Hit
GCTCAA2657890.8874216333033407No Hit
CGAAGC2574520.8595858908277305No Hit
GGATCG2559000.8544040421624856No Hit
TTAGCG2377620.7938445247074518No Hit
CGAGCC2370420.7914405742957401No Hit
ACTAAT2314150.772653034064211No Hit
CGCTTG2234200.7459591680341638No Hit
GAAGAT2212550.7387306226989478No Hit
GCTGTC2112930.7054692976969053No Hit
TATAAG2096620.7000236822503754No Hit
GCGGCT2068350.6905848380643912No Hit
GGTACC2068040.690481334643887No Hit
CGAGAG1995720.6663349882862508No Hit
GCCTGG1990530.6646021406978087No Hit
ACACGA1945940.6497143422452784No Hit
CATGAG1935840.6463421340288495No Hit
TATTGG1921470.6415442496654753No Hit
GTTGGC1758450.587114805760306No Hit
TCCACG1705980.5695960171349579No Hit
GAGCCA1644790.5491657774554258No Hit
CAGGAC1131120.37766060967988696No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)