FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17268905
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGG4077652.361267260431394No Hit
TACGGC4002402.3176918281732397No Hit
AACTAG3832362.2192258281576045No Hit
CCTGAC3572462.0687241026573484No Hit
TGACAC3061451.7728107254049983No Hit
GTTACA2969561.7195994766315523No Hit
CGTACT2958771.713351251859918No Hit
ACAGCC2926541.6946876481166582No Hit
TACAGT2900571.6796490570768672No Hit
TTAGTA2680441.5521771646783626No Hit
TATGGT2572681.4897759875336625No Hit
ACGTAA2512071.4546782207673272No Hit
TTCACA2497811.4464206039699679No Hit
CCATGA2467251.4287240563313077No Hit
CACCTA2424651.4040554395313427No Hit
TGCGAC2399901.38972332061587No Hit
GAGTGA2389331.3836024924568175No Hit
AGCCTA2369471.3721020527937353No Hit
CATAAC2334511.3518575729034354No Hit
TAGAGC2301871.3329565482003636No Hit
GCTCAA2284261.3227590284386879No Hit
CTTCAC2274651.3171941127709024No Hit
GTACTT2269101.313980243680766No Hit
GGCAGG2231581.2922533304804213No Hit
GAGTCG2209801.2796410658348054No Hit
TCTTCA2209371.2793920633647589No Hit
CAAGGA2183661.2645040319580194No Hit
CAACGG2179561.2621298223599007No Hit
AGATGC2165451.253959066889302No Hit
GTCTCA2069101.1981651413335124No Hit
CTTAGC2067501.1972386205147345No Hit
CCTTAT2055351.1902028530471387No Hit
GCATAA2047761.1858076699130604No Hit
GAATAC2041121.1819626085151316No Hit
GCACAT2019111.1692171565018163No Hit
ATGCCG2007161.162297204136568No Hit
AGGTGT1985481.1497428470421258No Hit
AGACGG1951901.130297491358022No Hit
AAGAGG1945251.1264466392049757No Hit
AGAACG1937851.1221614804181272No Hit
AACTCT1933431.119601966656253No Hit
ACCTGC1925541.1150330608686538No Hit
AGTAGG1908791.1053335460470712No Hit
TACTCG1902361.101610090506607No Hit
CCGACT1896701.0983325231101797No Hit
AGGCTT1852661.0728300375733146No Hit
GGTAAT1839351.0651225425121047No Hit
CCAGCG1838311.0645203039798992No Hit
TCCAGA1801381.0431350453314787No Hit
AGTTCC1757071.0174762094064447No Hit
GGCCGT1720370.9962241381257236No Hit
TCTCGC1717090.9943247704472287No Hit
CCTGCT1655190.9584799962707536No Hit
TCGTGG1605180.9295204299288229No Hit
CAAGCT1582300.9162711822202971No Hit
GGTGGT1533710.8881339031050319No Hit
CTACCG1527990.8848215911779004No Hit
GGCGTT1497920.8674087905399909No Hit
AAGCGC1491130.8634768678153015No Hit
CTCCGC1487150.8611721472785913No Hit
CAGTAG1471660.8522022676017964No Hit
TTAGCG1446460.8376095647060424No Hit
ATGGCC1434180.8304985174219212No Hit
GTCGAG1430480.8283559380284969No Hit
CCGGAA1410810.8169655227126446No Hit
ATGTGA1390560.8052392435999851No Hit
AGAGGT1273700.7375684792984848No Hit
GCGCAG1235860.7156562619343844No Hit
CGTCTC1201630.6958345071676519No Hit
TCAAGC1145590.6633831154899514No Hit
GGCCAG1145090.6630935777340833No Hit
AAGCAT1099190.6365140117453886No Hit
AGGTCA1095240.6342266634740303No Hit
TGGCAT1044250.604699603130598No Hit
AACCTT976360.5653861666388228No Hit
TAATGA923250.5346314662105096No Hit
CTATGC899550.5209073765823601No Hit
AACAAT889780.5152498088326967No Hit
TAAGTC851930.49333180071347893No Hit
ACGAAC793800.45967014121624966No Hit
CTATTA714190.41356993972692535No Hit
GGATCG690760.40000220048694457No Hit
ATCCGG690480.39984005934365846No Hit
TATTGG662010.3833537795245269No Hit
GATCCT651670.3773661387331739No Hit
CTGTTC630850.36530978657882474No Hit
CATACG568620.3292739174834768No Hit
GAAGAT532290.3082361041420982No Hit
GAGCCA529030.306348317973838No Hit
TGCATA523340.3030533783120586No Hit
CGAGAG500430.2897867583381807No Hit
TCCACG333370.1930464033475197No Hit
ACACGA329190.1906258677084621No Hit
CGAGCC312140.18075263023335875No Hit
GCCAAT297310.17216494039430988No Hit
GGTACC269570.15610138569874582No Hit
TGACCA254870.14758897567622267No Hit
CAGGAC219890.127332914275688No Hit
ACAGTG206920.1198223048884686No Hit
TTAGGC182470.10566390862651684No Hit
ACAAGT174830.10123977171685176No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)