FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001836470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31687813
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGG7271032.294582462980326No Hit
AACTAG6929262.186727118087954No Hit
TACGGC6825392.153947954691603No Hit
CCTGAC6488462.047620010885573No Hit
TGACAC5586121.7628606934785938No Hit
CGTACT5307941.6750730004623544No Hit
GTTACA5245821.6554692493293874No Hit
TACAGT5223371.648384506687161No Hit
ACAGCC5209861.6441210379523512No Hit
TATGGT4610381.4549378967870077No Hit
ACGTAA4555631.4376599609446066No Hit
TTCACA4523091.4273910288475888No Hit
TTAGTA4518251.4258636277612469No Hit
CCATGA4446221.403132491346121No Hit
CACCTA4419181.3945992423017644No Hit
TGCGAC4363681.377084622406728No Hit
AGCCTA4331701.3669924144023446No Hit
GAGTGA4284091.3519677107410346No Hit
CTTCAC4242051.3387007806439655No Hit
CATAAC4226761.3338755817575672No Hit
TAGAGC4171411.3164082986730576No Hit
GGCAGG4119331.2999729580580397No Hit
GTACTT4103961.2951225128726933No Hit
GCTCAA4102901.2947879994116351No Hit
GAGTCG3950951.2468358103476562No Hit
TCTTCA3942711.2442354415560328No Hit
AGATGC3940321.2434812083749673No Hit
CAAGGA3901631.2312714670463374No Hit
CAACGG3882731.2253070289199195No Hit
GTCTCA3772271.1904482016477438No Hit
CTTAGC3705381.1693391399400141No Hit
CCTTAT3684421.1627246096156905No Hit
GCATAA3656311.1538536913229072No Hit
ATGCCG3640871.1489811556259815No Hit
GCACAT3634771.1470561253312117No Hit
GAATAC3614681.1407161485079453No Hit
AGGTGT3595861.1347769566804753No Hit
ACCTGC3546291.1191337186949444No Hit
AGAACG3522341.1115756079474466No Hit
AGACGG3497031.1035883101178363No Hit
AACTCT3485641.0999938683051431No Hit
TACTCG3466061.093814836637669No Hit
AAGAGG3441701.0861273386080637No Hit
CCGACT3436911.0846157164585641No Hit
AGTAGG3402121.0736367322036393No Hit
AGGCTT3356691.059299990188657No Hit
CCAGCG3288521.0377869876977626No Hit
TCCAGA3245231.024125584179634No Hit
GGTAAT3233921.0205563886658886No Hit
AGTTCC3144350.9922900012064575No Hit
TCTCGC3130360.9878750546779608No Hit
CCTGCT2966660.936214815456024No Hit
TCGTGG2913050.9192966393736293No Hit
GGCCGT2902710.9160335552346259No Hit
CAAGCT2873590.9068439024176266No Hit
CTACCG2781120.8776623366213376No Hit
GGTGGT2734780.8630384179558243No Hit
GGCGTT2706620.8541517207261984No Hit
CAGTAG2639230.8328848696500449No Hit
CTCCGC2622980.827756715176273No Hit
AAGCGC2583180.8151966814497422No Hit
GTCGAG2580930.8144866292918354No Hit
TTAGCG2578180.8136187877655047No Hit
ATGTGA2553990.8059849381211635No Hit
CCGGAA2519860.7952142358325581No Hit
ATGGCC2505880.79080244509143No Hit
TCAAGC2366670.7468707291348886No Hit
AAGCAT2265040.7147984621090764No Hit
GCGCAG2208460.6969430171782446No Hit
AGAGGT2199060.693976577051878No Hit
CGTCTC2165740.6834614935401191No Hit
TGGCAT2108940.6655366212871806No Hit
GGCCAG2066210.6520519418616867No Hit
AGGTCA1952780.6162558457410741No Hit
TAATGA1951450.6158361260210669No Hit
CTATGC1865510.5887152893763921No Hit
AACCTT1798850.5676788107781373No Hit
TAAGTC1727090.5450328806219602No Hit
CTATTA1651500.5211782839036572No Hit
AACAAT1622220.5119381384887622No Hit
GGATCG1528270.48228951616193894No Hit
TATTGG1524390.48106507066297066No Hit
GATCCT1468340.46337688246266795No Hit
ACGAAC1464860.46227866845843857No Hit
ATCCGG1264730.39912189585314706No Hit
GAGCCA1198360.378176935088578No Hit
CATACG1156450.364951030227299No Hit
CTGTTC1154660.364386144288342No Hit
CGAGAG1145130.3613786789261853No Hit
GAAGAT1002470.31635821632752No Hit
TGCATA959660.3028482906030782No Hit
TCCACG639570.20183469272555982No Hit
ACACGA638490.20149386768976452No Hit
GCCAAT636710.20093213753817596No Hit
CGAGCC589160.18592636860107703No Hit
NNNNNN529500.16709894116075477No Hit
TGACCA524720.16559047479862368No Hit
GGTACC506190.15974280080483938No Hit
ACAGTG424250.13388427910755468No Hit
CAGGAC418760.13215175184226188No Hit
ACAAGT370160.11681462523147307No Hit
CGATGT335110.10575359050496796No Hit
TTAGGC334340.10551059487759536No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)