FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16419742
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGG3884172.365548740047194No Hit
TACGGC3787242.306516143798118No Hit
AACTAG3660752.2294808286269054No Hit
CCTGAC3375542.055781388038862No Hit
TGACAC2883701.7562395316564658No Hit
GTTACA2792701.7008184416052334No Hit
CGTACT2790971.6997648318712926No Hit
TACAGT2724831.6594840527944958No Hit
ACAGCC2702151.6456714118894193No Hit
TTAGTA2521541.5356757737119133No Hit
TATGGT2439861.4859307777186754No Hit
ACGTAA2384561.4522518076106188No Hit
TTCACA2373721.4456499986418787No Hit
CCATGA2328321.4180003559130223No Hit
CACCTA2294091.3971534997322126No Hit
TGCGAC2288271.393608986060804No Hit
GAGTGA2263701.3786452917469714No Hit
AGCCTA2236901.3623234762153997No Hit
TAGAGC2214171.3484803841619435No Hit
CATAAC2191681.3347834576207105No Hit
GCTCAA2176821.3257333763222345No Hit
GTACTT2168611.3207332977582718No Hit
GGCAGG2161451.3163726933102848No Hit
CTTCAC2160041.3155139709259744No Hit
GAGTCG2138351.302304262758818No Hit
TCTTCA2078121.2656228094205133No Hit
CAACGG2053301.2505068593647817No Hit
CAAGGA2045621.2458295629736449No Hit
AGATGC2045241.2455981342459583No Hit
GTCTCA1995341.21520788816292No Hit
CTTAGC1945161.1846471156489549No Hit
ATGCCG1936831.1795739543288803No Hit
CCTTAT1934801.1783376377046606No Hit
GCATAA1927331.1737882361367187No Hit
AGGTGT1909901.1631729658115213No Hit
GCACAT1901011.157758751629593No Hit
GAATAC1881261.145730548019573No Hit
AGACGG1864541.1355476840013685No Hit
AGAACG1853381.1287509876829978No Hit
TACTCG1829851.1144206772554648No Hit
ACCTGC1829721.1143415042696774No Hit
AAGAGG1819321.1080076654066793No Hit
AACTCT1815741.1058273631826858No Hit
AGTAGG1814701.105193979296386No Hit
CCGACT1789991.0901450217670898No Hit
AGGCTT1754381.0684577138909979No Hit
CCAGCG1747751.0644198916158367No Hit
GGTAAT1743431.061788912395822No Hit
TCCAGA1698081.0341697208153453No Hit
AGTTCC1669571.0168064760091846No Hit
TCTCGC1631640.9937062348482699No Hit
GGCCGT1572550.9577190676930247No Hit
CCTGCT1560530.9503986116225213No Hit
TCGTGG1545890.9414825153769164No Hit
GGTGGT1479670.901153014462712No Hit
CAAGCT1474900.8982479749072794No Hit
CTACCG1468460.8943258669959613No Hit
GGCGTT1436920.8751172825979848No Hit
CTCCGC1416870.8629063720976857No Hit
CAGTAG1400390.8528696735917045No Hit
TTAGCG1395250.8497392955382612No Hit
GTCGAG1383070.8423213957929424No Hit
AAGCGC1347430.8206158172278224No Hit
ATGTGA1338720.8153112271800618No Hit
ATGGCC1327820.8086728768332657No Hit
CCGGAA1322940.8057008447513975No Hit
GCGCAG1181570.7196032678223567No Hit
AGAGGT1174410.7152426633743697No Hit
CGTCTC1149220.6999013748206275No Hit
TCAAGC1141140.6949804692424522No Hit
GGCCAG1120850.6826233932299302No Hit
AAGCAT1087600.6623733795573645No Hit
TGGCAT1044920.636380279300369No Hit
AGGTCA1037410.6318065168137234No Hit
AACCTT945630.5759103888477662No Hit
TAATGA939080.5719212884100128No Hit
CTATGC908200.5531146591706496No Hit
TAAGTC860250.5239120078744233No Hit
AACAAT826970.5036437235128298No Hit
ACGAAC771690.46997693386412526No Hit
CTATTA758690.46205963528537775No Hit
GGATCG755580.4601655738561544No Hit
TATTGG719270.43805194990274515No Hit
GATCCT699210.4258349491727702No Hit
ATCCGG677060.4123450904405197No Hit
CATACG625170.3807428886519654No Hit
CTGTTC615230.3746892003540616No Hit
GAGCCA565100.3441588789884762No Hit
GAAGAT548300.33392729313286407No Hit
CGAGAG534680.32563240031420715No Hit
TGCATA510530.3109244956467647No Hit
ACACGA350070.2132006702663172No Hit
TCCACG346930.2112883381480659No Hit
CGAGCC313900.19117230952837141No Hit
GCCAAT310730.18924170672109222No Hit
GGTACC282300.1719271837523391No Hit
TGACCA268980.16381499782396092No Hit
CAGGAC228460.1391373871769727No Hit
ACAGTG218280.13293753336684583No Hit
TTAGGC194850.11866812523607252No Hit
ACAAGT182250.11099443584436344No Hit
CTTGTA170830.10403939355441762No Hit
CGATGT167660.10210879074713843No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)