FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21698939
Sequences flagged as poor quality0
Sequence length6
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCT4851432.235791344452372No Hit
GTAAGA4333621.9971575568740942No Hit
TTACGT4241841.954860557928662No Hit
GTACGC4118931.8982172354141371No Hit
GAGATA4086351.883202676407358No Hit
GAGAAG3998301.8426246555188712No Hit
TGATAA3622521.6694456811920622No Hit
ACGCTA3413841.573275080408309No Hit
ATTGCT3396921.5654774641285456No Hit
ATTGAA3392911.5636294475043226No Hit
GGAGGC3324281.5320011729605767No Hit
ATTCGA3277311.5103549532997904No Hit
AATTGC3201571.47545002085125No Hit
CGTGAA3156541.4546978541208857No Hit
TTGAAT3104541.430733548769366No Hit
GGACTA3079561.419221465160117No Hit
ATCCAC3060181.4102901528964158No Hit
CTGGAG2933341.3518356819197472No Hit
GTCTGC2903521.3380930745047026No Hit
CGTCAG2867911.3216821338591715No Hit
TATTCT2841501.3095110318527554No Hit
TGTGAT2836891.3073865040129382No Hit
CTGTAT2792661.2870030189033665No Hit
CTGAGG2777731.2801224981553245No Hit
TTCCAT2740671.263043322072107No Hit
AGTCAA2722561.254697291881414No Hit
ATTCAT2718731.2529322286218696No Hit
CACATT2697331.2430699952656672No Hit
CTAGTT2667551.2293458219316622No Hit
CTCGTC2646081.2194513289336404No Hit
GGATTC2641261.217230022168365No Hit
AAGGCG2605611.2008006474417943No Hit
CCACTT2599161.1978281518741538No Hit
TTCTGT2595861.1963073401883844No Hit
ATACCT2553291.1766888694419575No Hit
ACTAAT2532021.166886546849134No Hit
CAGTCC2522471.162485410000922No Hit
CGAATT2463241.1351891445014892No Hit
TCGGAC2413311.1121788028437705No Hit
ACCAGG2393931.1032474905800693No Hit
GATTCA2386191.0996804958989008No Hit
GACCAT2380461.0970398137899737No Hit
AGTGCA2368601.0915741087617232No Hit
TCTCTG2364141.0895187087258045No Hit
GACTAA2342731.0796518668493422No Hit
GCCATA2283091.052166652019253No Hit
TGCGGA2269441.0458760218644791No Hit
ATCAGC2260781.0418850433193991No Hit
TTCTAC2218881.0225753434303861No Hit
GACTTG2201251.0144505222121691No Hit
AGGACT2156850.9939886922581791No Hit
AACATA2147810.9898225899432225No Hit
ACTAGC2140830.9866058428018071No Hit
GTGGTG2124230.9789556991703604No Hit
GACACG2059440.9490970964064187No Hit
TCATGT2047150.9434332250069923No Hit
CCGTCG2029020.9350779777757797No Hit
ACCGCT2027290.9342807037708157No Hit
GAGCAC2008050.9254139107907533No Hit
GCTGTC2000220.9218054394272457No Hit
GCGTAT1998980.9212339829150172No Hit
TCCTCC1978050.9115883500110306No Hit
TGTGTG1974020.9097311163462877No Hit
AGTTAG1962210.9042884539193368No Hit
CCGCTC1940520.8942925734755971No Hit
CGTTGC1912910.8815684490379921No Hit
TACGAA1902130.8766004641978118No Hit
CGACAA1888680.8704020044482359No Hit
GCCTGG1874150.8637058245105901No Hit
CATTAA1874120.8636919989498105No Hit
TATAAG1854300.8545579117946736No Hit
AATGTA1844310.8499540000550257No Hit
GCTTCT1840310.8481105919510626No Hit
AATCGG1768360.8149522886810272No Hit
CGCTTG1762630.8123116065721001No Hit
GTTGGC1751890.8073620558129593No Hit
GACCGA1720950.793103294128805No Hit
GAACCG1707880.7870799581491058No Hit
TCAATG1632300.7522487620247239No Hit
AAGTTG1591520.7334552164048205No Hit
GCGGCT1455810.670912987957614No Hit
CATGAG1450620.6685211659427219No Hit
CGAAGC1432060.6599677523403333No Hit
TTCGTT1159380.5343026218931718No Hit
NNNNNN289680.13349961488900447No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)