FastQCFastQC Report
Wed 27 Sep 2023
EGAF00001836490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384352224
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGC49764741.2947691438361495No Hit
AGTGCA40982261.066268319550559No Hit
GGCTAC40902591.064195481278131No Hit
CTATTA40381161.0506290188657788No Hit
CTTCAC40190381.0456653426311382No Hit
AGATGC35646420.9274414917916541No Hit
TAGCTT34728390.9035563691703784No Hit
TCCTCC33942780.8831165238684817No Hit
ACGCTA32730330.8515712400300823No Hit
GAGCCA31786750.8270213625718478No Hit
CTCCGC30983110.8061124163028128No Hit
TAAGTC29980370.7800233256878462No Hit
ACTTGA28659990.745669940497079No Hit
CATAAC28498310.7414633822959223No Hit
CTGTAT28344540.7374626249073037No Hit
GACCAT27500180.7154942337474285No Hit
CATTAA27488700.715195549382329No Hit
CGAATT26780500.6967697421207064No Hit
GATTCA26676030.6940516623626978No Hit
ATTGAA25461100.6624418543757405No Hit
GATCAG25310340.6585194105706541No Hit
CAGATC25174660.6549893152172837No Hit
CACCTA25159790.6546024305039535No Hit
AGGCTT24927850.6485678615456638No Hit
AAGCAT24717220.6430877319445406No Hit
TGGCAT24502420.637499108109753No Hit
TTCACA24381320.6343483523071796No Hit
CCGACT24204040.6297359163973512No Hit
CAGTCC23990810.6241881405114492No Hit
TCTCGC23950020.62312687437448No Hit
AGACGG23817240.6196722306464395No Hit
GTTACA23565140.6131131428030971No Hit
GCCAAT23521280.6119720020144855No Hit
GCTGTC23017810.5988728193231425No Hit
ACCGCT22262060.5792098655841263No Hit
TTCTAC22164050.5766598608259907No Hit
CAGCCT21936310.5707345666354203No Hit
TATTCT21824250.5678190117614618No Hit
GTACTT21674500.5639228459362317No Hit
AATCGG21659900.5635429860293978No Hit
GATCCT21630520.5627785830113995No Hit
GCTTCT21517370.5598346687334376No Hit
GTACGC21345670.5553674121578649No Hit
GGCGTT21293680.5540147466403108No Hit
CCGTCG21252820.552951659257213No Hit
TTCCAT21251400.5529147139786031No Hit
TATGGT21237740.552559310805497No Hit
AGGTGT21181860.5511054360387935No Hit
CACATT20891940.5435623549299405No Hit
GGTAAT20569150.5351640686746748No Hit
GCATAA20469800.5325792000620764No Hit
ACCTGC20455690.5322120888781432No Hit
ACCAGG20005360.5204954921764678No Hit
GAGTCG19854920.5165813740679694No Hit
GCGTAT19727980.5132786743026626No Hit
TCTCTG19269380.5013469103797874No Hit
CCGCTC18970810.4935787752850365No Hit
TCGTGG18907650.4919354909209528No Hit
GTCTGC18840110.49017824858481895No Hit
TCTTCA18540100.48237264785542133No Hit
GGTGGT18514380.4817034699921497No Hit
CAAGGA18376460.4781150947626623No Hit
ACGTAA18362570.4777537074951334No Hit
CAAGCT18334770.47703041260403894No Hit
ATTGCT18282360.475666819609713No Hit
CCTCGG18066920.47006154438174913No Hit
GAGAAG18026680.4690145880357909No Hit
GAACCG17975430.46768117569159684No Hit
CCAGCG17930770.4665192206615149No Hit
TGCGAC17675990.4598904051092469No Hit
GACACG17416600.45314164749050595No Hit
AATGTA17344070.4512545763232009No Hit
CCGGAA17260010.44906751990070437No Hit
CGTACT17109970.4451638089129413No Hit
CCTTAT17087440.44457762783753263No Hit
TGTGTG17074790.44424850264428284No Hit
TCGGAC17064270.4439747953689478No Hit
TGTGAT17051720.4436482719558818No Hit
TTACGT17003020.44238120500637457No Hit
CGTGAA16980090.44178461681023085No Hit
AGTCAA16964330.441374576253265No Hit
AGTTCC16964160.4413701532269526No Hit
GTGGTG16939000.4407155453327103No Hit
GACCGA16648390.4331545119405891No Hit
TCAATG16625120.43254907769182055No Hit
CAACGG16576990.43129684088936093No Hit
ATGTGA16576740.43129033643890147No Hit
GAATAC16559660.4308459523835096No Hit
TACAGT16532220.4301320239010768No Hit
CCACTT16525620.4299603064089464No Hit
CAGGAC16519330.42979665443538584No Hit
CGTCTC16497360.42922504332900646No Hit
GTAAGA16449830.4279884172076497No Hit
TGCGGA16411990.4270039035861023No Hit
TATAAG16286340.42373476678516625No Hit
CTGAGG16225350.4221479410510709No Hit
GCGCAG16186290.42113168571128134No Hit
AACTCT16123890.4195081748765944No Hit
GGACTA16101340.41892147344514913No Hit
ATACCT16051660.41762890904984074No Hit
AAGCGC15989040.41599967429874946No Hit
TTCTGT15902500.41374809372769494No Hit
GTCTCA15898520.41364454287638No Hit
GGAGGC15891260.4134556536350366No Hit
CGTTGC15854010.41248649051657366No Hit
AGCCTA15662470.40750304075253635No Hit
TAATGA15545640.40446338096381096No Hit
AACAAT15451330.4020096420724757No Hit
ACTAAT15348610.3993370934676835No Hit
TTGAAT15199820.39546590473221765No Hit
GAGCAC15192530.39527623495681913No Hit
GAGATA15178580.39491328662118No Hit
GTCGAG15129330.393631909880662No Hit
TTCGTT15079850.39234454904572114No Hit
CTACCG15031380.39108346619063666No Hit
CGATGT14963630.3893207601161168No Hit
TATTGG14840000.38610417927489343No Hit
ATCCAC14763990.3841265661571923No Hit
AAGAGG14738530.3834641529223986No Hit
GGATCG14610970.38014532211995217No Hit
ATCAGC14504500.37737520675826763No Hit
TCCAGA14309900.3723121425206063No Hit
GGTACC14286480.37170280560156194No Hit
AGAACG14283320.3716205893477541No Hit
TGATAA14202690.36952277398556177No Hit
GGCAGG14006400.3644157396627943No Hit
CTGGAG13975050.36360008157517515No Hit
ACTAGC13968030.3634174366062729No Hit
CGACAA13937750.3626296175666204No Hit
CGTCAG13840550.3601006872279735No Hit
AAGGCG13831290.35985976238295425No Hit
TCATGT13821690.3596099914853101No Hit
TACGAA13760290.3580124984524612No Hit
GACTTG13616820.3542797244227732No Hit
GACTAA13482520.3507855336359391No Hit
GGATTC13433700.3495153445502113No Hit
TACTCG13433110.34949999404712695No Hit
ACAGTG13291700.34582081668922515No Hit
CTAGTT13267340.34518702303645316No Hit
CGAGAG13028820.3389812569420699No Hit
AGGACT13010210.338497065649866No Hit
AACCTT12960910.337214388019256No Hit
TTAGGC12838370.33402616658203593No Hit
GAAGAT12799510.333015114802614No Hit
ATGCCG12755130.3318604447570466No Hit
CTCGTC12739210.33144624135178674No Hit
AGGTCA12664140.32949308496781327No Hit
AAGTTG12578420.32726283899426584No Hit
CAGTAG12393320.32244694387406486No Hit
TAGAGC12285520.3196422248359359No Hit
AACATA12265240.3191145838146627No Hit
ACAGCC12216770.31785350095957815No Hit
CTTAGC12187710.3170974236381679No Hit
GCGGCT12181030.3169236247218905No Hit
CGAAGC12010120.3124769222097698No Hit
CATGAG11934390.31050659407658326No Hit
ATGGCC11856120.30847018072672844No Hit
CTATGC11818830.30749997689619196No Hit
TTAGCG11773900.30633099705961375No Hit
CATACG11729080.3051648791812377No Hit
GCTCAA11715980.3048240459771608No Hit
GGCCAG11538090.3001957392082113No Hit
ATCCGG11520950.29974979408470914No Hit
GCCATA11511240.29949716122886283No Hit
CCTGCT11263250.29304500655107435No Hit
AGTAGG11085020.28840785372949995No Hit
ATTCAT11069710.2880095211833612No Hit
GAGTGA11060380.2877667750922133No Hit
TACGGC10975470.2855576035381546No Hit
TTAGTA10923860.284214824785299No Hit
TGACCA10908760.2838219559775462No Hit
AGAGGT10759430.27993671762908806No Hit
CCTGAC10749580.27968044228098443No Hit
ACACGA10569230.2749881317195136No Hit
GGCCGT10241410.26645897592100315No Hit
GTTGGC10161980.2643923819210163No Hit
CGCTTG10144370.2639342084306503No Hit
GCCTGG10136360.2637258058379285No Hit
AACTAG10068830.26196882367981306No Hit
CGAGCC9848780.25624360638537635No Hit
GCACAT9752550.2537399133145123No Hit
TGACAC9466960.24630948928761762No Hit
ATTCGA9091720.23654656932595242No Hit
AATTGC8846380.23016336182303448No Hit
AGTTAG8785840.22858824409976614No Hit
CCATGA7723890.20095863943797554No Hit
CTGTTC7636840.19869378978798363No Hit
TCCACG7291590.18971114370343803No Hit
TGCATA7029930.18290332567452502No Hit
ACGAAC6204910.16143811880219536No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)