FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001836492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381610056
Sequences flagged as poor quality0
Sequence length6
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATC41453681.0862837430049277No Hit
GCACAT38840981.0178185660809735No Hit
TTACGT33377950.8746611750713404No Hit
GGCTAC33323570.8732361602127172No Hit
CAGTCC32278360.8458466828243121No Hit
GGCCGT31086090.8146035334037424No Hit
CTCCGC29446850.7716476423252326No Hit
CGTACT29273510.7671053091955208No Hit
AATCGG28941860.7584145004816121No Hit
TGGCAT27799800.7284870920697122No Hit
GGATTC27764520.7275625881305392No Hit
TATAAG27565590.7223496751878048No Hit
TATGGT27523180.7212383313085439No Hit
GAGAAG27300750.7154096065015645No Hit
ACCAGG27240370.7138273630818576No Hit
TGCGAC27075280.709501219223636No Hit
AACCTT27063490.7091922651011062No Hit
ATCCGG27019150.7080303460346967No Hit
GAACCG26708840.6998987469030428No Hit
TGTGTG26069580.6831470919099679No Hit
CTATTA25910370.6789750320416085No Hit
ACCTGC25903370.678791598720344No Hit
GAATAC25591610.6706220026864281No Hit
ATTCAT25342380.6640909903066077No Hit
GGTACC24863640.6515457234177289No Hit
CGAGCC24729720.6480363819343378No Hit
AGATGC24571700.6438955057305932No Hit
TATTGG24516830.6424576505394816No Hit
GAGTCG24500270.6420236997108902No Hit
TCTTCA24180150.6336350318818642No Hit
CGTGAA23638680.619445940386854No Hit
CCGTCG23215080.6083456039743356No Hit
TACGAA22975720.6020732325774979No Hit
GACTAA22932700.6009459037945268No Hit
ACTTGA22929590.600864406990365No Hit
CCGCTC22792860.5972814301308663No Hit
ACCGCT22708450.5950694863240187No Hit
GGTAAT22335840.5853053306331111No Hit
AGGACT21887970.5735690046910085No Hit
GACACG21493250.5632254617525069No Hit
CCGACT21283420.5577269169238035No Hit
AGTGCA21164750.5546171980331671No Hit
TTAGCG21078410.5523546790391709No Hit
GATTCA21023270.5509097485628104No Hit
TAGAGC20874140.5470018326770718No Hit
CTTCAC20773600.5443672060885104No Hit
TCAATG20587200.5394826387908394No Hit
CAGGAC20447060.5358103036991247No Hit
GTTACA20402580.5346447159662899No Hit
GATCAG20203040.5294158181198453No Hit
TAGCTT19998450.5240545862344885No Hit
TAAGTC19898980.5214479987393205No Hit
TTCTGT19867420.5206209765080195No Hit
AGAACG19759810.517801082264981No Hit
TGTGAT19607670.5138142900510986No Hit
ATCCAC19567420.512759548453828No Hit
CCTGAC19521090.511545481914659No Hit
AACTCT19405840.5085253833038405No Hit
GCATAA19322260.5063351894479426No Hit
AGTCAA19120840.5010570266523584No Hit
TACAGT18971490.4971433457193801No Hit
TTCGTT18887550.49494371814981736No Hit
GCTGTC18606670.48758332511027963No Hit
GTAAGA18595850.4872997896051251No Hit
ATACCT18431940.4830045673639166No Hit
CTAGTT18362040.48117285462729004No Hit
TTGAAT18181330.47643739241504685No Hit
GTACTT18119850.4748263237591412No Hit
CATACG18101880.47435542421869514No Hit
TATTCT17916420.46949548939559393No Hit
AATTGC17852020.4678079028399608No Hit
ACGCTA17755450.46527730914931653No Hit
CGCTTG17664430.46289215187767485No Hit
GCCAAT17586420.4608479185359832No Hit
CCACTT17492690.458391746364252No Hit
CAAGCT17477670.45799815086633877No Hit
AACTAG17339560.4543790114377908No Hit
TTCTAC17320030.453867232471463No Hit
TTCCAT17315510.45374878695544646No Hit
TACTCG17284780.4529435146750954No Hit
TCGTGG17249250.4520124595458774No Hit
CTGAGG17156490.44958170599152136No Hit
ATCAGC17155230.44954868799369374No Hit
AACATA17008640.445707332198814No Hit
CGAATT17002840.44555534458976626No Hit
ACTAAT16697340.4375497903545812No Hit
TGATAA16610500.4352741689804946No Hit
TCTCTG16489290.43209788999899934No Hit
GTTGGC16486970.43203709495538034No Hit
CCAGCG16398080.42970775382292337No Hit
ATGCCG16242190.42562269375836365No Hit
CCTCGG16187080.42417854942480865No Hit
TCCTCC16132600.42275091409016746No Hit
CGTCTC16090400.42164507321054456No Hit
TCTCGC16051030.42061339180223284No Hit
GACCGA16042010.42037702486540346No Hit
TGACAC16025850.4199535559408843No Hit
AGGTGT15952980.41804401506652117No Hit
AGTTCC15939750.41769732608933136No Hit
TTCACA15870260.4158763573043788No Hit
AGCCTA15803890.41413714737118984No Hit
TCCACG15587820.4084750848389593No Hit
GTGGTG15486100.4058095366333847No Hit
GACCAT15427330.40426948287756864No Hit
GACTTG15427220.40426660035394874No Hit
AGGTCA15376360.4029338262511614No Hit
GTCGAG15310770.4012150560309134No Hit
TCGGAC15295590.40081726777137133No Hit
GCGTAT15153480.3970933093021008No Hit
GATCCT15104240.3958029869108062No Hit
AACAAT14935720.39138696072516493No Hit
GAGCAC14902290.3905109355923262No Hit
GGAGGC14779960.3873053072794287No Hit
TAATGA14682690.384756370256658No Hit
CATAAC14553360.381367308622496No Hit
CGATGT14488970.37967998411446474No Hit
CAGCCT14454040.37876465184135505No Hit
AAGTTG14391030.37711348990237303No Hit
ATTCGA14335130.3756486438082753No Hit
CCGGAA14264450.3737964913587078No Hit
GCTTCT14249560.3734063024796181No Hit
GGCGTT14195530.3719904592870582No Hit
AAGCAT14182880.3716589690707731No Hit
GTCTGC13855540.36308110287324297No Hit
AGACGG13805330.3617653618645731No Hit
CTGTTC13421660.35171138152606757No Hit
GGTGGT13391430.3509192116258068No Hit
CGTTGC13266010.3476326106039512No Hit
CAACGG13142070.3443847926271628No Hit
GGATCG13138750.34429779282336315No Hit
CATTAA12918990.338539034726066No Hit
TGCGGA12856740.3369077884048213No Hit
CCATGA12851550.33677178569948374No Hit
TGCATA12849230.3367109906558647No Hit
GAGTGA12819190.3359237996600383No Hit
ACACGA12703020.33287959266985356No Hit
CCTTAT12609920.3304399294970361No Hit
GCGGCT12596570.3300900959486246No Hit
CAAGGA12565680.32928063090664467No Hit
CTCGTC12517380.32801494098991985No Hit
TCAAGC12505330.32769917362974316No Hit
GAAGAT12501940.3276103394927308No Hit
ATTGCT12449580.3262382582496725No Hit
CAGTAG12439680.3259788311238842No Hit
GCGCAG12371570.32419402490798094No Hit
GGCCAG12354110.32373648979522696No Hit
AGAGGT12227620.32042184967997805No Hit
CTGTAT12192030.3194892222651491No Hit
TTAGTA12152300.31844810714317234No Hit
ACGAAC12150140.3183915048611822No Hit
GCCATA11799740.30920935689388646No Hit
CACCTA11743260.307729312038884No Hit
CTTAGC11694120.30644161012360743No Hit
AAGAGG11692980.3064117366970015No Hit
AAGCGC11676180.30597149672596674No Hit
AGGCTT11600590.30399067890391235No Hit
ACAGTG11431030.2995473997676833No Hit
AATGTA11039240.28928063677651095No Hit
TCATGT11035570.289184465306648No Hit
GAGCCA10876560.2850176463903247No Hit
CATGAG10874000.28495056220426224No Hit
ACAGCC10835500.2839416789373077No Hit
CTACCG10742110.2814944163840378No Hit
AGTTAG10733530.28126957954168796No Hit
CGTCAG10704030.28049653911635913No Hit
TGACCA10703000.28046954821337305No Hit
AGTAGG10671050.27963230612560164No Hit
GAGATA10644670.27894102455203645No Hit
CGAGAG10639740.27881183508434587No Hit
TCCAGA10609300.27801416218444724No Hit
ACTAGC10447360.27377056332079464No Hit
CTATGC10378420.2719640071539415No Hit
GCCTGG10369770.27173733597837896No Hit
GTACGC10205810.2674408034991615No Hit
GGCAGG10050270.26336491510066495No Hit
TACGGC9983730.2616212503582453No Hit
ATGTGA9969760.26125516985852176No Hit
CTGGAG9728120.2549230516084723No Hit
GCTCAA9710580.25446342011490386No Hit
ATGGCC9605880.25171978172399107No Hit
CGACAA9528360.24968838871478796No Hit
ACGTAA9182750.2406317615487575No Hit
CCTGCT8933460.23409917688332615No Hit
GTCTCA8888250.23291445967555952No Hit
TTAGGC8785410.23021956213858263No Hit
CACATT8562580.22438035542753096No Hit
GGACTA8222380.21546549601407777No Hit
CGAAGC8186610.21452815174241635No Hit
ATTGAA7351360.1926406258015381No Hit
AAGGCG6758150.1770957000147816No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)