FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415511316
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAGT96813992.3299964711430388No Hit
GGACCGGC93115552.240987102262216No Hit
CCGGTAGG88760442.136173831665273No Hit
TCGGATTG83970942.0209062128165964No Hit
CGATGCTC82133651.9766886445037275No Hit
TGCAGTGT79896411.92284558623188No Hit
ATTCTGGC77053001.8544139962724866No Hit
TTATGCTA76088401.8311992253900493No Hit
AGTTACAT74574621.7947674859473621No Hit
AGAGCCGC74387871.79027302351496No Hit
ACTCATAG72662071.7487386552909188No Hit
GGCCGTCA71236411.7144276763812614No Hit
AAGCAGGA70175881.6889041837791972No Hit
TTAAGGAC69222611.6659620889843587No Hit
GAACAAGT66627971.6035175802528563No Hit
CAGGCTAG66244791.5942956894102978No Hit
TTCACGGT65879781.585511091110693No Hit
GAGGTATA63515731.5286161303967953No Hit
ATAAGCCA62272181.4986879442773107No Hit
TCGTGAAG58295271.4029767121913954No Hit
TACTCAAT58023791.3964430754516441No Hit
TGTTGCGT55784231.3425441823586821No Hit
TGAGCGTA54960181.3227119908330005No Hit
CAGTGGTC52435411.2619490247529144No Hit
TCGATTGC51988991.2512051537003146No Hit
TAATTAGA46929701.129444570891061No Hit
CTTGCTTG46751611.1251585263685093No Hit
GAACAGAC42471471.0221495387625015No Hit
GCCTGGTC42428411.0211132252292259No Hit
GGATATGC41244440.9926189350761268No Hit
CCACATAT41079340.9886455174183512No Hit
GCTGCATG40884010.9839445624147575No Hit
TCCGGCGA40178980.9669767934791937No Hit
AGAAGTAG40072920.9644242757518547No Hit
AGAAGAGC39854530.9591683418797672No Hit
CGTACACT39177890.9428838275008616No Hit
CAGTACCG38945480.9372904780287621No Hit
TGCTCGAG38074790.9163358140648087No Hit
AGGATCTT36950020.8892662745194646No Hit
GTTAAGCC36498140.8783909991033795No Hit
TAGGTCTC36195720.8711127376372103No Hit
CCGGCTGA35311840.8498406334618334No Hit
TGCCTGAC34946920.8410582011682204No Hit
TGCTCAGC34926360.8405633891328245No Hit
AAGTGGAG34899620.839919844686011No Hit
ACACTGTG34259360.8245108780623438No Hit
GCGGAGTA33615770.8090217692169905No Hit
CTCCAATG33521730.8067585336231854No Hit
CAAGAGAG32553850.7834648238557238No Hit
ATGTTGTG31939090.7686695589296538No Hit
GCATAACG31869050.7669839249335871No Hit
CTTACAAG27895640.6713569264140089No Hit
TCTCTATA27656290.66559655381323No Hit
GCGAGATA23237580.5592526389822798No Hit
GGAACCTG22912170.5514210833189439No Hit
GGAAGGCG20788570.5003129686123879No Hit
GCATCCGT16972050.4084617998706923No Hit
CGCATATA16877050.4061754602129776No Hit
ATCGGCGC15430640.37136509658860895No Hit
GGCCTAAG15086280.36307747633039195No Hit
TAGATCCG14349260.3453398125985093No Hit
CCATCTCC13605740.32744571510057263No Hit
GATTACGC12776020.30747706519742535No Hit
GATGGCGT12702610.3057103263103429No Hit
GGATCTCT12578760.3027296613986802No Hit
CCAGCGTG12578670.3027274953926887No Hit
AGGCCGAC11812290.28428323237290604No Hit
CTAGCGGC11244170.2706104398851077No Hit
CAAGACGC10869110.2615839709164503No Hit
CGTAATGT10123900.2436492006393395No Hit
AACCAACG9703220.23352480730031427No Hit
TGCTAACT9458070.22762484764674856No Hit
GGAGTGCA9416390.2266217462053428No Hit
TACGAGCG9314940.22418017611823596No Hit
CCGCTGTA9257180.22279008160634547No Hit
TTAGCAGT8560770.20602976791130279No Hit
GCAGTTAA8477560.2040271750384772No Hit
GCTGTAGC8445950.20326642560078914No Hit
CCACTACA8386520.20183613964438935No Hit
ACCTCGCA8293780.1996041908037951No Hit
GTAGCACA8153390.19622546212435765No Hit
AGTGTCTC7776720.18716024571518527No Hit
TCGGGTTG7771760.18704087471831934No Hit
CTCTCGAA7548080.1816576278273971No Hit
CATTCCAG7326710.17632997509025722No Hit
TCGTGGAG7324060.1762661982471736No Hit
GACCTGTG7098630.17084083457308297No Hit
ATACTTAG6970470.16775644204115972No Hit
GGCTGGGT6945050.16714466568222175No Hit
AGGCGGTT6810730.16391202207354563No Hit
TAACATTA6636900.15972850183459264No Hit
CTGTCATT6601660.1588803901552467No Hit
ACGCATTA6446190.15513873513856358No Hit
GTGCGGTG6433310.15482875561444398No Hit
TGTATATT6406320.1541791944843206No Hit
GAAGGTGA6295520.15151260044142817No Hit
AGCCACGC5875650.14140770115632664No Hit
GGTCGTAT5827910.14025875531149193No Hit
CTTCTAGA5796330.1394987278758011No Hit
TGTAGACG5744470.1382506270900213No Hit
CGTATGAC5513440.1326904897097917No Hit
TAACGGTC5513200.1326847136938143No Hit
GCATGTAT5368730.12920779274276128No Hit
CGGTGCTC5368140.12919359337015024No Hit
GTCATACA4868280.11716359609325296No Hit
CCGGTGGG4764000.11465391715107948No Hit
TGCGGTGT4325180.10409295327109697No Hit
GGGCCGGC4248310.10224294348700723No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)