FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001836498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411383361
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCACTT95050372.310505941926028ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TACTCCGG92428142.2467641806251857No Hit
TGGCTGTG90141002.1911678630094134No Hit
GTTGCCTT89555382.1769324792890687No Hit
GCGTACGA86015942.090894969376265No Hit
CGTGATCC85746382.084342443786879No Hit
GCCATGCG83217572.022871557024398No Hit
ACCTAGGT80392281.9541937672097536No Hit
GTTATCCG79906031.9423738919766373No Hit
CAGGCGCA76363251.856255192586654No Hit
TCTATGAA73976461.798236560180177No Hit
CATCTCCG73508841.7868695472104912No Hit
AACGGATA73302881.7818630248392568No Hit
ACACTAGC73163281.7784695963918677No Hit
TGACGGAA72707911.7674003591992629No Hit
CTGGATGC72686231.7668733568443962No Hit
ATAACTGA66968881.6278947169183149No Hit
ACAGGCTG66740981.6223548720532721No Hit
TAATACCA65974911.6037330688248228No Hit
CGAGAGGA62661771.5231965106143415No Hit
TGTCCTGC55975011.3606532326425327No Hit
TCGTCGAC53936861.3111094203929168No Hit
TCCGGTCT50513491.2278933663532396No Hit
ACTGAGGC50326291.2233428663149066No Hit
CATTGTGA47430771.1529579097390865No Hit
AATCGCGG46749281.1363920963249654No Hit
GAAGGCAG45950061.116964475381395No Hit
GTGTCGGC45406921.1037617051312876No Hit
CATACGTT44615291.084518583628374No Hit
GAGTCCTA43504291.0575121437641226No Hit
GCGACAAG41283671.0035328093884672No Hit
CACAGGCA41178241.0009699930474338No Hit
TCCTCCTA41110630.9993265138402134No Hit
AACAGTGA41006870.9968042922377698No Hit
GCAGATCT40293260.9794576985820289No Hit
TGAGCTCC39844950.9685600774699296No Hit
CTCTGAGA39456320.9591131713273159No Hit
TTAGGATC39176490.952311000249716No Hit
GTAATCTT39173100.9522285953612012No Hit
CAATCGTA38657610.9396979475793626No Hit
GCACTCTC37122420.9023802010310281No Hit
GTATCTAG36902680.8970387112958611No Hit
CAACAGCA36251860.8812184311946444No Hit
TACATGTC35682700.8673831608857899No Hit
CGCCTCAT33847200.8227654107770296No Hit
AAGGCCTG33295400.8093521312836958No Hit
ATACCGAA32501070.7900433775686907No Hit
TTACAGCG30786690.7483698398778943No Hit
TCGGCAGC27110610.6590108538687349No Hit
TTCCGGTT25831440.6279164995202613No Hit
ATTAATCA24639900.5989522750775523No Hit
GTACTATA24018610.5838498169108012No Hit
GTAGCTTC22008860.5349963582994792No Hit
CGGCACTC21203610.5154221587489047No Hit
GCACCACT19820610.4818038812221187No Hit
TTCTTGCC19113340.46461140172365895No Hit
TTGTGTAC18623470.45270353070988695No Hit
TAAGACTG17667920.429475804686228No Hit
TTAACCAG17352330.4218043714218184No Hit
CCATTGAC17312990.42084808578342087No Hit
GAGTGAAC16721610.40647268667728154No Hit
CTGAACTA16671010.40524269040623645No Hit
TGCTTCTT16469220.40033753334034333No Hit
TGATCCAT16360580.3976966875916014No Hit
ACCATAAG15508340.3769802444683707No Hit
TAACCTCT15430720.3750934399118782No Hit
ATCATCTC15217380.36990752282759437Illumina PCR Primer Index 7 (100% over 8bp)
GCAGCTGG15107800.367243827345754No Hit
GGTCCGTG14962410.3637096542657689No Hit
GGCAACAC14924470.3627874001447521No Hit
AATGCGAA14562030.3539771264594243No Hit
TAATAGGC14416690.3504441687907742No Hit
CAGATAGT14294600.34747637739291065No Hit
ATAATATG13984670.3399425286916259No Hit
ATGGCGCT13767190.33465597554880205No Hit
GTTGTGTG13329350.3240128615702568No Hit
TAGCCACC13160020.31989674954306185No Hit
GGCACTCC12553000.3051411697713267No Hit
GCGCCTAA12378560.30090084270569223No Hit
GGTGTACG12253250.29785477881785305No Hit
AGCAAGCT12102710.2941954183703604No Hit
GAGCCGTC12010060.29194326116655944No Hit
AGAACACA11968280.2909276634550127No Hit
GTAATTCC11954090.29058272971813265No Hit
TTACCTAC11831060.28759208858717067No Hit
ACAGCGGA11739360.28536302419873516No Hit
CTAGTCCG11331780.275455477160147No Hit
AGTTCGTC11308510.2748898247248264No Hit
CAGGAATC11015630.26777043128878514No Hit
GCTTCTCA10105770.24565334814307185No Hit
GACTAAGA9686010.23544972690327162No Hit
TGGCTTCC9343210.22711686679034157No Hit
TGAGTAAG8860700.21538790432508523No Hit
ACCGAACT8405960.20433398131530167No Hit
TCGTGTCA8295600.2016513254166349No Hit
CCGAAGGT7938260.19296502368747967No Hit
CGGACATC7904090.19213441158112374No Hit
TCTGCGCA6669420.16212177332082228No Hit
ATATGGTT6524190.15859148955710925No Hit
CCGAATAG6309010.1533608453356965No Hit
CTGACTGG5168030.12562564483496452No Hit
GCGTACGG4280380.10404844740426922No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)