FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001836500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417538418
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTA90882122.1766169550414878No Hit
CGCTTACC74367941.781104128243356No Hit
TCCGACTT71183981.7048486302402954No Hit
CGAGGAGT63720311.526094540119659No Hit
GACTTATC60686541.4534360763899814No Hit
GCGCGAGG59428211.423299208840706No Hit
TCGGCAGC54213871.2984163292011133No Hit
GGACACAT53234921.2749705824674558No Hit
TGTGAACA53136381.272610560113776No Hit
ATTAATCA49346031.1818320871254535No Hit
GTCTCCAC47199861.130431547498942No Hit
TGTGGAGC46237411.1073809739826146No Hit
TATGGTGG46174171.1058663828151019No Hit
TCACGCCG45900341.0993081838998586No Hit
GGTAGCGA44926641.0759881740989878No Hit
CAGAGTGC44546381.0668809881825054No Hit
AAGTGAGA44235581.0594373617615231No Hit
CTCGTATC42681291.0222122841879426No Hit
TCAGGTAC41299690.9891231134568317No Hit
TGTCGGCT38825230.9298600637989676No Hit
TACAGCCT38640460.9254348422616286No Hit
CGTGTGGT37773280.9046659749522737No Hit
CCTGGCCA37549190.8992990436630911ABI Solid3 GAPDH Forward Primer (100% over 8bp)
TTATGACG37075830.8879621228051882No Hit
GTCCGACT35886440.859476360807594No Hit
TCTCGAGT35765650.8565834533578178No Hit
TATCTACT34611120.8289325845939283No Hit
GAGTATAA34398100.8238307786087364No Hit
CTCACCTT34183230.8186846653234193No Hit
CTCACGCC34091990.8164994771810435No Hit
GAACGCGC33822990.8100569562439642No Hit
GATCGATT33382520.7995077473326059RNA PCR Primer, Index 42 (100% over 8bp)
TCAAGGTT33234390.7959600498366595No Hit
CCGCGTAC33198840.7951086311775027No Hit
AGACCATC33032670.7911288776306088No Hit
GCCGGTTG32942310.7889647653931572No Hit
CGCACCGA32801010.7855806456592935No Hit
TCTGACCG32606660.7809259841569837No Hit
AGAACGGT32286510.7732584262461808No Hit
GCTGGAAT31626540.7574522160497337No Hit
TGATTCGC31547200.7555520316216746No Hit
CTGTAAGG31422630.7525685935802918No Hit
GTTGCAAC31282720.7492177641962517No Hit
AGCTCACG30725150.7358640229364475No Hit
AAGCTCCA30645200.7339492290742932No Hit
CTAATGAT30477620.7299357061797365No Hit
TTGACCGA29858330.7151037775881979No Hit
TCGTTCTG29839130.7146439396625773No Hit
ATGAATTG29538050.7074331061914403No Hit
CTGTAGCA29189580.6990872873403472No Hit
TCCGCGAT29002060.69459620360012No Hit
AATCGCGG28650910.6861861990385756No Hit
TCCAACCA28512370.6828681810065199No Hit
ATTCGATC28280540.6773158775535716No Hit
TCGCAACA28252280.6766390536067989No Hit
AGCGAAGA27950530.6694121737080491No Hit
GAGCTTAG27829110.6665041778263383No Hit
ACCGTTCA27763160.6649246824516157No Hit
TCGAGCAA27083980.6486583948306285No Hit
AATCTGTA26649540.6382536037677855No Hit
GGTAATTA26409170.6324967682375038No Hit
TAGGATCA26287820.6295904488482303No Hit
CGCGTCGG26263860.629016609436883No Hit
AACACGTA26078650.624580849947082No Hit
GTGTCGGC25940180.6212645084074635No Hit
GACGCCTC25439820.6092809404666566No Hit
CTCCTTCA25421950.6088529558973421No Hit
GTGGACAC25316920.6063374987448461No Hit
ATATACAA25270510.6052259842590102No Hit
TATCAAGA25051770.5999871848918104No Hit
TCACCGAG24616710.5895675448959525No Hit
CACACATG24282350.5815596590204066ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
TACACACA24123730.5777607271578061No Hit
CGAATCGT24010810.5750563053577504No Hit
ACTTGTCC23965540.5739720937487482No Hit
CGGAGAGA23860740.5714621450714027No Hit
ATCTCAGT23262000.5571223867596299No Hit
ATGCTAGG22760640.5451148689268637No Hit
AGCATAGT22424800.5370715372112178No Hit
ACTCTTGT21946830.5256242073513819No Hit
AATTGCTA21882840.5240916537648999No Hit
AGCTTCAC21024020.5035230075523254No Hit
GATCTTCC20976090.5023750892307113No Hit
TTAGAGAT20534570.4918007329327957No Hit
TCTAGTGA20337040.4870699107740548No Hit
TTCGCATA20115440.48176261471585113No Hit
ACGGACGG20009220.47921865719192336No Hit
TCAATAAC20005740.4791353115679046No Hit
CGATGGCA19788420.47393052104728717No Hit
TAGAGTAG19583840.46903085215023255No Hit
GTAGAATG19338620.46315785964394773No Hit
TTAATACT18808490.4504613034195096No Hit
CGACTCTG18427470.4413359155851378No Hit
TCGGCCAG18411610.44095607029866174No Hit
ACATACTC17942860.4297295584426916No Hit
GTTCAGGA17148720.41070999124205143No Hit
CTGACTGG16973050.4065027137215431No Hit
ATAGGTAT16940660.40572697672097807No Hit
CGTTCATA16815750.40273539571632905No Hit
CAATATGG16164250.38713204110477806No Hit
TTATCGTC16144580.38666094672993656No Hit
GGCCGCTC16125670.38620805427298427No Hit
CTTAGATT15635790.37447548119991203No Hit
ATTGACCT15444410.3698919508767215No Hit
TCGGTAAT15358250.3678284281854993No Hit
GTGAGTCA15110080.3618847834979343No Hit
CTACAAGC14986000.3589130809036116No Hit
AGCGTGCG14731030.35280657695072265No Hit
TTACAATT14657010.3510338059478877No Hit
TCCTTACA13413350.3212482833136567No Hit
TTCGGAAG13308160.3187289941784471No Hit
AGGTAGCC13134880.31457895689972176No Hit
ATGCGGCA13007740.3115339676360033No Hit
AATGGTCT12073530.28915973906860953No Hit
ACGCGACC11913000.2853150629123666No Hit
ACGCTATT11885800.28466362585107075No Hit
GCCGTCCT11642910.2788464365930514No Hit
CCGACCGC11112630.26614628788481925No Hit
AGGATTGG10568440.25311299617943184No Hit
GGATACTA10245940.2453891560225244No Hit
GTCAGATG8994590.21541945871912554No Hit
CGTGCAGG8553630.2048585143607073No Hit
TCGTCTGG8523430.2041352276235333No Hit
GATGTCCA7893390.18904583769343114No Hit
TGATCCAT7752480.18567105841743167No Hit
CAACCGGT7656370.18336923430121346No Hit
GGTCAACT7625610.18263253562454224No Hit
ATAATATG7622860.18256667342165386No Hit
ATAACGCG7073670.16941363225646938No Hit
CAATTAAG7045420.16873704780861626No Hit
AACTTGAA5800830.13892925177486304No Hit
GAGGTGCG5574310.1335041222482191No Hit
CTGATCCT5403350.1294096487188396No Hit
TTGCGTCG4930570.11808661879827308No Hit
GTATGCCT4598220.11012687220556551No Hit
CGGCCAAG4445160.10646110174225931No Hit
AGGTGTAA4368390.10462246853653596No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)