FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001836502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415676078
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTATT40555520.9756520075711453No Hit
CGAGCCTT39507080.9504294832189021No Hit
CAGCTAAC38844690.9344942385642889No Hit
TCGCGGAT37777770.908827137269131No Hit
ACCTGAAC35654760.8577534740885425No Hit
ATCTGTCT35190120.8465755395238309No Hit
TAGGCATT35162670.8459151695518067No Hit
CTGCTCAG34822120.8377224921757465No Hit
GCGCTGGC34247230.8238922519856915No Hit
CGTTGTCT33881570.815095498471288No Hit
GTGGTCGT33645070.8094059721185111No Hit
TACGTACC32595320.7841519328422839No Hit
CTCAGAAC32496040.7817635346338117No Hit
TGTGAGTC31898290.7673833469916448No Hit
GATTGGAT31827290.7656752862261177No Hit
GCCTACAT31769980.7642965684448169No Hit
CACTGCTT30741280.7395489330997778Clontech Universal Primer Mix Long (100% over 8bp)
ACTCCGTT30636550.7370294231846558No Hit
CGCCGGTA30105310.7242492795074918No Hit
ATGTGCAT30047750.7228645474277209No Hit
GATACGAG29981280.7212654657504731No Hit
TATATAGC29589990.7118521263569082No Hit
GACGATGT29562630.7111939215323332No Hit
ATATTCGG29409960.7075211097425722No Hit
ACGTGGTA29391360.7070736459363919No Hit
ATGGTCAA29190690.702246088840359No Hit
TGGAGTTA28984910.697295599483596No Hit
CAATCAGC28944030.6963121413977544No Hit
GTGGCGAA28834350.6936735483729233No Hit
AGGAATAC28732710.6912283751868925No Hit
TAACTATG28719500.6909105796557289No Hit
GTCCTCAA28694990.6903209378337138No Hit
TATACTAT28673000.689791920140278No Hit
TTGTCACA28603910.688129808615063No Hit
TAGTCGTG28381510.6827794886960034No Hit
GGTTCAAT28350860.682042135703561No Hit
CGCTGAAG28208240.6786110987123007No Hit
TTGGAACG28207990.6786050844138305No Hit
GGAAGAAT28012380.6738992567188338No Hit
GCCTTAGG27906820.6713597793327909No Hit
CTTGCGAT27869290.6704569128464497No Hit
GGCGTGTC27745070.6674685282225936No Hit
TACTGCAC27694410.6662497907805991No Hit
AGCGCTTC27590710.6637550597751742No Hit
TTGTGGCT27527290.6622293525392625No Hit
GTCGAAGC27186430.6540292174330995No Hit
CTTCCTGT27145640.6530479244947072No Hit
TATTAGAG27005110.6496671670386575No Hit
GCAGTGGT26782960.6443228614180679Clontech Universal Primer Mix Long (100% over 8bp)
TACTGTTG26733640.6431363606158735No Hit
CTTAACAC26719590.6427983570418503No Hit
CTCTTAAT26647360.6410607059278499No Hit
CGTGTTAG26033970.6263042637733894No Hit
GATATTGT25999110.6254656299947094No Hit
TGCCAGGT25788980.6204104918445655No Hit
ATAGTTGC25768770.6199242959562373No Hit
AGGCGTGC25755830.6196129958674216No Hit
ACTTCTAT25514480.6138067921243233No Hit
CGGAGCTG24901200.5990529962611897No Hit
CTAACATA24720150.5946974413090954No Hit
CAACGTAA24681780.5937743667798945No Hit
TATCCGAC24608260.592005681885788No Hit
AGGTTATC24393010.5868273709029751No Hit
CTTGCCGA24240240.583152153393826No Hit
GACTCGGT23855070.5738860440268106No Hit
TTGAAGGC23741900.571163491395336No Hit
AGATGACT23661650.5692329015864127No Hit
ACCACCAA23645230.5688378824628921No Hit
AGTCTACC23637550.5686531232138887No Hit
ATTGCCAG23634350.5685761401934706No Hit
TTGCGTCG23620460.5682419857704681No Hit
GTTACCAA23502820.5654118974823468No Hit
GGAATATC23260010.5595705702361828RNA PCR Primer, Index 37 (100% over 8bp)
CGCTACAA23199530.5581155911502802No Hit
TCGCACGC23192750.5579524833757694No Hit
ATCCTAAC23149420.5569100851649202No Hit
GTCCAGAG23031620.554076147725778No Hit
TGCAATCG22894800.5507846424590255No Hit
TCATTGCG22821530.5490219718633893No Hit
TCCGTATG22763660.5476297820535153No Hit
CACATTAT22600610.5437072565912731No Hit
CGTGCCAC22514980.5416472390792717No Hit
CTATCCTG22382740.5384659157604927No Hit
AATTAACA22197060.5339989760007311No Hit
TCATTCAA22183560.5336742038833421No Hit
CGCAAGTG21903590.5269389113125726No Hit
TAGCGCTG21864880.526007657337452No Hit
GATACACC21772280.5237799611841026No Hit
CTCGAACA21717890.5224714904089333No Hit
GGCTCTTA21714070.5223795919283092No Hit
GCGGAAGT21588530.5193594518085306No Hit
GTCCATCC21383370.5144238779119735No Hit
TCTCCACG20891610.5025935122492182No Hit
CGCGATTA20830320.5011190468362723No Hit
GATATATG20721120.4984920012645038No Hit
AGTGGATG20216500.4863522600884432No Hit
CTATGGAG20160260.48499928350459465No Hit
GATCGGCG20154310.4848561432010047No Hit
TGGAAGAT20105360.4836785435605462No Hit
GAGTGGCA20081680.48310886920945206No Hit
TATAGGTG19894370.47860271622366496RNA PCR Primer, Index 39 (100% over 8bp)
AGGCCAGA19871500.47805252819961414No Hit
GGATTGTG19864590.47788629298989876No Hit
GCGCAATC19321770.46482756700759675No Hit
GACAGTCG19195820.46179756343832706No Hit
CCGACACA19119420.4599595938258444No Hit
CGTCGTGG18803190.452351987404962No Hit
TAGCATGG18760520.45132546694207404No Hit
ATAGGTAT18569860.4467387223567867No Hit
CAAGCACT18496260.44496811288716975No Hit
ATGTCAAC18359230.4416715556097024No Hit
AACTACCT18256610.4392028063736687No Hit
CGGATGCG18235460.43869399672309267No Hit
CCTACTCG18113320.43575565106250835No Hit
CAATACAT18034240.43385320817042544No Hit
AAGCCTGT17965790.432206493249294No Hit
GGAGAACC17577350.4228617168582889No Hit
GTGGTACC17519610.42147265448361937No Hit
GCTCACGT17410100.4188381511817478No Hit
TCCGATGC17313880.4165233679865503No Hit
TCTGACCG16479520.3964510077002796No Hit
ATCGCTAA16037890.3858266291667619No Hit
TGGCGCGA15807830.38029203114257637No Hit
CGACCTCG15635510.37614649549306034No Hit
AATGGTCT14989340.3606014585231917No Hit
AACGTCGA14883120.3580461033891876No Hit
ATGCTAGG14862930.35756038864473694No Hit
AGATGGAC14670730.35293659598087335RNA PCR Primer, Index 16 (100% over 8bp)
TCGTCTGG13861550.33346999583651765No Hit
TCGGTAAT13794700.3318617724255953No Hit
GGCCGCTC13698100.32953784749672316No Hit
CCAGATTC13634810.32801526769601597No Hit
GAGACTGA13616200.32756756331789677TruSeq Adapter, Index 15 (100% over 8bp)
TGTACCTA13473020.3241230542980633No Hit
CCGACCGC12949180.3115209338556163No Hit
CGTTCATA12913280.31065728059530046No Hit
ATTGACCT12435030.2991519276218729No Hit
CAGATTCA12030690.28942464184816524No Hit
TTATCGTC11885440.2859303344369988No Hit
GTCATTAG11816140.2842631709010688No Hit
TTACAATT11654720.28037985866485204No Hit
CGACTCTG11326150.2724753864714823No Hit
TCTAGTGA10825150.2604227323372696No Hit
CTACAAGC10211970.24567134219352407No Hit
AGGATTGG9902750.23823237670174519No Hit
TTAATACT9267170.22294210541507273No Hit
GGATACTA8923040.21466330328491984No Hit
AACTTGAA8875570.2135213082914047No Hit
TCGCGGGT4978280.11976344715223183No Hit
GATTGGGT4707620.11325212705649132No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)