FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001836504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416803478
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACGCG54853711.3160569163964606No Hit
TCTCTATA52353641.256074931313313No Hit
AGGCGTGC48333531.159623960719445No Hit
TTCACGGT46837491.123730786142816No Hit
TGTAGACG46524401.1162190925863629No Hit
CTCACCTT45796731.098760744985914No Hit
TGTGGAGC44833781.0756575308615826No Hit
GGACCGGC44293331.062690988389497No Hit
ATAGTTGC44116731.0584539795994696No Hit
AGGTGTAA44029251.056355148744704No Hit
CGACAGTT43482281.0432321776355236No Hit
TTCCGGTT43248541.0376242589799118No Hit
AGCGAAGA42255761.0138053598487486No Hit
TGATCCAT41315480.9912460471359118No Hit
GGATCAGA40968920.9829313372476225No Hit
CTTGCGAT40945410.9823672824534347No Hit
CCTTAGCC40924640.9818689660742227No Hit
AACGCTGG40809760.979112751069702No Hit
CATCTCCG40198250.9644413283902589No Hit
GATGTCCA39881710.9568468620120295No Hit
TAGTCGTG39788150.9546021590539607No Hit
CTCTATAG39498890.9476621977708161No Hit
ATTGAGTA39494350.9475532735357837No Hit
CAGGCTAG39241850.9414952626666901No Hit
AATCGCGG38737500.929394835807968No Hit
AGCGCTTC38596470.9260112268065095No Hit
CTTATGTA38476510.9231331318209394No Hit
CTGTAGCA38440230.9222626976255702No Hit
TTCGTTCG38357670.9202819080122935No Hit
GGCCTGCT37123550.8906727500963895No Hit
TGCCTAGA36952850.8865772948276597No Hit
GTCAACGT36944750.8863829586374037No Hit
CGAGGAGT36124790.8667103780741484No Hit
TATATAGC35246400.8456359378076015No Hit
AACTCTAC34962820.8388322517788587No Hit
CCATCTCC34605940.8302699431889098No Hit
AGTTCGTC34043900.8167854107973639No Hit
CGAGCCTT33827180.8115858380625125No Hit
TGTGAACA33713820.8088660910838177No Hit
TAGGATCA33394610.8012075657391707No Hit
CGCTGAAG33389620.8010878450491241No Hit
ATAGGAGG32980750.7912781860231982No Hit
ATAAGCCA32913610.7896673549350758No Hit
CGGCCAAG32053060.7690209341295372No Hit
TCTATGAA31539580.7567014591946375No Hit
AAGACACT31501080.7557777624878649No Hit
TCCAACCA31388510.7530769692857505No Hit
TATTAGAG30688970.7362935200843022No Hit
GTTATCCG30560250.7332052541077885No Hit
ATGTGCAT29947350.7185004823784125No Hit
TTCGCATA29408330.7055682486411498No Hit
CCACTACA29321280.7034797343989534No Hit
TCGTTCTG29271100.7022758097042558No Hit
AGTTCCGG28895650.6932679674040532No Hit
CGTTGTCT28798990.6909488888669975No Hit
TACGTACC28788650.6907008103228928No Hit
TCTCCACG28613620.6865014691648038No Hit
GATACACC28444450.6824427218431225No Hit
CCGGCTGA27893520.6692247419298166No Hit
TATCTACT27042200.648799768412681No Hit
TAGATCCG27023660.6483549544660949No Hit
CGCACCGA26924900.6459854924723061No Hit
TTATGACG26850220.6441937607823897No Hit
AATTGCTA26747630.6417324089603685No Hit
CAGTACCG26564010.6373269754721193No Hit
GACCTGTG26222940.6291439823350035No Hit
AGCAAGCT25802600.6190591336668261No Hit
GTCCGACT25795540.618889749283714No Hit
ACTAAGTG25696040.6165025331194572No Hit
TACAGCCT25492370.6116160575800186No Hit
CATTGTGA25068460.601445557035395No Hit
TCGGTAAT24886700.5970847488945378No Hit
CAGATAGT24436660.5862873341954215No Hit
TGCTCAGC24329480.5837158585322554No Hit
TAACCTCT24074780.5776050649942033No Hit
CGTATGAC24001960.575857958651679No Hit
CTCTGAGA23597470.5661533851212249No Hit
GCAGTGGT23113280.5545366394471402Clontech Universal Primer Mix Long (100% over 8bp)
GAGTATAA22659280.5436442159438986No Hit
AGACCATC22391400.5372172062345411No Hit
TACATGTC22026020.5284509646054345No Hit
ATCATGGA21623470.5187929357921528No Hit
CGTTCGCG21593130.5180650148029714No Hit
GGTGCGGA21281740.5105941078543496No Hit
TAGCTGGT21265910.5102143125590713No Hit
GTCTCCAC21038580.5047601834071068No Hit
CATAATAA20864240.500577396813373No Hit
GATCGGCG20504710.4919515090994514No Hit
ATTCGATC20039650.4807937327240825No Hit
ATAGGTAT19600520.47025807207875553No Hit
AGGTAGCC19261640.4621276216893756No Hit
ACTCATAG19146050.45935437227805476No Hit
GCTGTAGC19038320.45676970094764896No Hit
GATATATG18185640.4363120981442482No Hit
CAAGAGAG17956370.43081142427511127No Hit
TCAATAAC17732130.4254314307809111No Hit
ATGCTAGG17555000.42118170616608913No Hit
AATTAACA17197470.4126038026966752No Hit
TCTCGAGT17111910.410551036716637No Hit
CGAATCGT16547740.3970154011046904No Hit
GCGCCTAA16377030.392919705914738No Hit
ATATACAA16370670.3927671160172037No Hit
CTTACAAG16199580.3886623038207949No Hit
TATGGTGG15796260.3789858010733778No Hit
TCGTGAAG15647040.3754056965906604No Hit
GGATCTCT15584800.37391242690158166No Hit
CGTAATGT15581370.373830133922251No Hit
CTAGCGGC15054300.36118460604592173No Hit
AGAAGAGC14604010.35038119331624196No Hit
ATCGCTAA14586430.3499594118070195No Hit
GCGGAGTA14536610.3487641242763334No Hit
CGATGGCA14313020.34339972566159827No Hit
CACACATG14280350.3426159030275654ABI Solid3 EF1 alpha Sense Primer (100% over 8bp)
CCGCGTAC14248240.34184551598199475No Hit
GTGGACAC14228510.34137215140992655No Hit
CGCGTCGG13810190.3313357668287019No Hit
TACTGTTG13687230.3283856954763703No Hit
GCGCAATC13292540.31891624474400376No Hit
CGCCGGTA12846940.3082253550676945No Hit
TCGTGTCA12308980.29531855298002097No Hit
GCAGTTAA12210170.29294789138011945No Hit
ATACTTAG11169790.26798696722967363No Hit
AGTCTACC10749580.25790523753738925No Hit
TGGCGCGA9866140.23670963705346046No Hit
AGGCGGTT9537420.22882294662617952No Hit
GTCCAGAG8988240.21564695292681796No Hit
AATGGTCT8724120.2093101535971348No Hit
GATCTTCC7835740.18799603202927206No Hit
CGTGCAGG4472230.1072982889072725No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)