FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001836506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413203735
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGCGTA50234221.215725216036588No Hit
CGCGCGCT49727811.2034695184930988No Hit
TGAGGCGG49242021.191712848384587No Hit
CTTAGATT49188031.1904062290240431No Hit
TCTGCGCA48022591.162201256481866No Hit
ATGTTGTG44875901.086047782215715No Hit
TACTCAAT44803841.084303848318312No Hit
GAGTGGCA44013031.0651653475494358No Hit
CAGGCGCA42466601.0277399840057109No Hit
CGCGTGAA41944301.0150997304029694No Hit
CAGAGGAT41862971.0131314519700554No Hit
CGATGCTC41850961.0128407963204884No Hit
GGAAGGCG40290250.9750698405473028No Hit
CTATCCTG39118440.9467107067655136No Hit
GAGAGCGG39020600.9443428675687068No Hit
CTCAGAAC39011110.944113198783162No Hit
TCGGATTG38721130.9370953532160111No Hit
GATGGCGT38715920.9369692652947582No Hit
ACCTAGGT38709770.9368204283051798No Hit
TAATTGAT37706370.9125370079241902No Hit
AAGCGCAC37592140.9097725120998724No Hit
CAGTGGTC36550510.8845638822698445No Hit
TGACGGAA36401830.8809656572925219No Hit
GCAACAGC36217250.8764986115142448No Hit
TCGCGGAT35885860.8684785968839318No Hit
CACTCTCG35715560.8643571433351153No Hit
ATCGCGTG35595640.8614549430440167No Hit
TGTCTTAA35512680.8594472167585804No Hit
TACTGCAC35378540.8562008763062126No Hit
TGCCTGAC35323940.8548794942523934No Hit
TAAGCAAC34977260.8464894442447379No Hit
CGTGCCAC34604680.8374725848012967No Hit
GAGCCGTC34399910.8325169180767448No Hit
CTGCTCAG34394210.8323789715985989No Hit
AGTGTCTC34292580.8299194100943933No Hit
CAAGACGC33483280.8103334303113209No Hit
ACTACATA33365990.8074948790092616No Hit
CCAGCCAA33240540.804458846433225No Hit
CTTAACAC32680190.7908977395860181No Hit
GCCTACAT32429920.7848409211499504No Hit
GGCTCTTA31778130.7690668623796443No Hit
CGCCTCAT31669000.7664257923515623No Hit
GGTGAGAT31527650.7630049617049081No Hit
GCTGCATG31419590.7603897868928993No Hit
CTAACATA31399810.7599110884125962No Hit
TAATTAGA31185110.7547151044024324No Hit
GTCTTGTA30690970.7427563548040049No Hit
CCACATAT30099650.7284457387588715No Hit
TAATGATT29919220.7240791276971396No Hit
GCCATCGC29791750.7209942088253389No Hit
TATCTACT29530290.7146665796716479No Hit
GGTTGGCC29241990.7076893920138452No Hit
CGTACACT29215310.707043705691576No Hit
GGTCAACT28698890.6945457547715536No Hit
TGCTCGAG28181330.6820202145559018No Hit
TGGCTTCC27990320.6773975554698217No Hit
GGTAGCGA27655340.6692906587594133No Hit
GGTTGCAG27579520.6674557285887069No Hit
TTAACCAG27519350.6659995462044892No Hit
GGATATGC26521160.6418422137447523No Hit
AGGTTATC26285890.6361484123564372No Hit
GGTGTACG26231940.6348427610413541No Hit
CGAACTGC25659420.6209871263627372No Hit
TCGCAACA24427280.5911679380148874No Hit
CATTAAGT24043460.581879057796997No Hit
GAACGCGC24032100.5816041328861656No Hit
GCGCTGGC24005040.5809492501320203No Hit
ATACCGAA23044200.5576958301211871No Hit
TCATTCAA22730530.5501046596299523No Hit
CTGGCCTC22105320.5349738670682636No Hit
GTCCAGAG21981800.5319845426857045No Hit
GCATAACG21917810.5304359119599924No Hit
GAAGGCAG21891130.5297902256377232No Hit
TTAGGATC21158090.5120498245254245No Hit
GTGGACAC20942860.5068410139129067No Hit
ACATTCCT20584450.4981670845739088TruSeq Adapter, Index 27 (100% over 8bp)
AGGCCGAC20072790.48578433106370633No Hit
CGGAGCTG19943830.4826633524984957No Hit
TGTATATT19716240.4771554158386298No Hit
GGCGTGTC19703600.47684951347305704No Hit
TAGCTGGT19640330.4753183075656371No Hit
TAGAGTAG19473460.4712798639150733No Hit
ATAGGTAT19401170.4695303637562715No Hit
TCGCACGC19331340.4678403983933011No Hit
CCGCTGTA19079580.4617475202638234No Hit
TGAGTAAG18892750.4572260219283836No Hit
AGCCACGC18524110.44830451496281853No Hit
CAACGTAA18478210.4471936827966959No Hit
AGTGGATG18415270.44567046326432647No Hit
ACCTCGCA18367050.4445034844614848No Hit
GTCATACA18148070.4392039195870289No Hit
TCGGTAAT18055780.4369703966978905No Hit
AATTGCTA18040450.4365993932750874No Hit
GTGTTAAG17776640.4302148914505819No Hit
GAGACTGA17352880.419959417840209TruSeq Adapter, Index 15 (100% over 8bp)
CATAATAA16889910.40875501766701117No Hit
CCGGTAGG16657760.40313672382462856No Hit
ATGCTAGG16561140.4007984100143722No Hit
ACAGGCTG16333070.39527885680897823No Hit
TCGTGAAG15968100.38644616801443No Hit
ATCTCAGT15805940.38252171171686045No Hit
GACTAAGA15795800.3822763121925798No Hit
GGTGCGGA15697990.37990919902986836No Hit
GAGGTATA15593120.3773712258433482No Hit
AGAAGTAG15101300.3654686228816397No Hit
ATCGCTAA14586760.35301617009826886No Hit
TCTCGAGT14479710.3504254384341419No Hit
CGTAATGT14454240.3498090354870582No Hit
GATATATG14148580.34241171609932325No Hit
TTACAGCG14094930.3411133251251952No Hit
ACCATAAG13973910.33818450358392815No Hit
ATTCGATC13943120.3374393505905749No Hit
TAACGGTC13814160.3343183720253642No Hit
GCACTCTC13812730.3342837644001451No Hit
ACTCATAG13607480.3293164811300653No Hit
CTTACAAG13569230.32839078765829643No Hit
AATTATTG13545970.32782786922291496No Hit
GGATCTCT13212550.3197587262854727No Hit
ATATACAA13171260.3187594613586927No Hit
ACGCATTA13003320.3146951224920559No Hit
TGTTGCGT12357020.29905392796122715No Hit
AATGGTCT12195640.29514834855014077No Hit
TACTGTTG11333240.2742772884180246No Hit
GCGGAGTA11143070.2696749582866186No Hit
TAGCATGG11009480.26644192845933495No Hit
CGCCGGTA10979320.2657120221819873No Hit
GTCGAAGC10668560.2581912769980165No Hit
AGAAGAGC10394500.25155871352421344No Hit
AATTAACA10380000.25120779704471935No Hit
CACATTAT10059940.243461981291142No Hit
AACACGTA9787980.2368802402040243No Hit
ATACTTAG9251630.2238999606332213No Hit
CGTGCAGG9207720.22283728872876718No Hit
TTGAGAGT9151470.22147597479969536No Hit
AGTCTACC9146210.22134867682161685No Hit
GATCTTCC8801880.21301549948477597No Hit
CCATTGAC8475160.2051085041619965No Hit
CGCGTCGG8352270.202134426495443No Hit
CGACCTCG8019390.19407835217171984No Hit
AGGCGGTT7376070.17850927702771127No Hit
CTCTCGAA6633450.16053702902758127No Hit
TATGGTGG6290160.15222902087271792No Hit
CTTCTAGA6118630.14807779992598566No Hit
AACTTGAA5485620.13275823849946564No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)