FastQCFastQC Report
Tue 8 Aug 2023
EGAF00001836508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419577841
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCGC49569111.1814043821251274No Hit
GCCACGAC43301971.0320366275014985No Hit
GCCTCATT42434451.0113606071012697No Hit
TTAAGGAC42171831.0051014586349427No Hit
ACCAGCGG42159191.0048002034502104No Hit
GGAGAACC40649940.9688295240548702No Hit
ATTCTGGC40611190.9679059767124356No Hit
ACTCCGTT40423960.9634436342885897No Hit
ATCTGTCT40068810.9549791739359277No Hit
GACGCTCA39590440.9435779522017227No Hit
AGTTACAT39402590.9391008330203978No Hit
CACGGCAA39330420.9373807707828881No Hit
TTGTCACA38550170.9187846981652209No Hit
TTGGAACG37949410.9044664968377106No Hit
ATCGGCGC37797400.9008435695725885No Hit
TTATGCTA37616760.896538289780656No Hit
CTTGCCGA37610370.8963859938447035No Hit
CTTCCTGT37077450.8836846557871487No Hit
GGCCGTCA36972950.8811940571475508No Hit
AGAGTATT36712730.8749921090327551No Hit
GCCTTAGG36548460.8710769833052266No Hit
AACGAGAT35889540.8553726267922714No Hit
TGATAAGG35888860.8553564200260042No Hit
CCAGCGTG35705760.850992509873752No Hit
AACTAGTC35416200.8440912874614844No Hit
GGTCCGTG35214120.8392750178625378No Hit
GAACAAGT34789770.8291612807073862No Hit
CTCTCTGC33041880.787502979691437No Hit
CTACGGCC32947480.7852530991978672No Hit
GCATGTAT32940830.7850946065571657No Hit
ACACTAGC32863820.7832591902774009No Hit
GGAGTGCA32751420.7805803071473453No Hit
GTCAGATG32695900.7792570723485848No Hit
TGTACCTA32161690.7665249891020818No Hit
ACATCCAG31940340.7612494483472972No Hit
CGCTACAA31212710.7439074934369568No Hit
AACCTTCT31212650.7439060634281686No Hit
GATATTGT30678860.7311839902431835No Hit
ACGAGTTC30613210.7296193222940007No Hit
TCGGCAGC30499550.7269104089793913No Hit
ACGTGGTA30408110.7247310755860437No Hit
GATTACGC30396910.7244641406122303No Hit
ATAATATG30334040.7229657297369048No Hit
TCACGCCG30115180.7177495343468341No Hit
TCGATTGC29644950.7065423171382399No Hit
GCGGAAGT29415230.7010672901574896No Hit
GTGGTACC29289520.6980711834112326No Hit
GCCTGGTC29262130.6974183843993801No Hit
GGTAATTA29257390.6973054137051056No Hit
TCCTTACA29221250.6964440717449614No Hit
ACTTCTAT29147760.6946925493141093No Hit
GACTCGGT29110870.6938133322441116No Hit
CTCTTAAT28960920.6902395019473871No Hit
AGGATCTT28453460.6781449642856616No Hit
TCCGGCGA28397530.6768119577601811No Hit
AATTCATT28338890.6754143625044298No Hit
CGGATGCG28157780.671097880976989No Hit
TGTGAGTC27988020.6670519094453322No Hit
GTGTCGGC27973380.6667029873009904No Hit
TGGAGTTA27588390.6575273359109544No Hit
TATACTAT27518980.6558730540777057No Hit
CTCGTATC27479280.6549268649294565No Hit
CCGGTCTT27436640.6539106053505814No Hit
CTTGCTTG27275070.6500598300185256No Hit
GTTAAGCC27079660.6454025297298768No Hit
GATTGGAT26420320.6296881631554037No Hit
CATTAAGT26207390.6246133003005752No Hit
GGCCTAAG26168570.6236880846145543No Hit
CGCGATTA25797480.6148437185938044No Hit
GTAATCTT25786040.6145710635848378No Hit
CTGGCCTC25773820.6142798184616237No Hit
GGCGGAAC25759620.613941383048396No Hit
CGAAGCAA25526690.6083898505974723No Hit
ACCACCAA24641120.5872836358867675No Hit
CTCGAACA24347630.5802887478988672No Hit
CAATCAGC24161450.5758514306288163No Hit
TCCTCCTA24060640.573448777529698No Hit
TTCGGAAG23756450.5661988713078868No Hit
CGTCCGAA23736550.565724585059772No Hit
TAACATTA23620070.5629484613321132No Hit
ATGAATTG22692540.5408421938088003No Hit
GATACGAG22317840.5319117889259553No Hit
AGATGACT22084400.5263481014003311No Hit
AAGTGGAG22000890.5243577675018353No Hit
GTCTGGCG21836840.5204478851398636No Hit
TTGAGAGT21463200.5115427437456117No Hit
TTAGCAGT21155060.5041986952785716No Hit
TCATTGCG21108200.5030818584149204No Hit
ACCTGAAC21010150.5007449857200633No Hit
GGAACCTG20665230.49252434186580407No Hit
GAGGTATA20638390.49188465126784425No Hit
AGCTGTGG20299250.4838017649268566No Hit
CGTGTTAG20122130.47958037898383676No Hit
GTTCTAAT20066890.4782638175594216No Hit
GGTTGCAG19764870.47106562998878676No Hit
GACAGTCG19721500.4700319719696542No Hit
ATGCGGCA19550100.46594691353111756No Hit
AGGCCGAC19152280.4564654785951863No Hit
TTGTGGCT18253090.43503465188954055No Hit
ACTTGTCC18071620.43070959030937No Hit
CAATACAT17847450.42536683914153606No Hit
TACGAGCG17715860.42223059153402714No Hit
TGGAAGAT17638220.4203801601619853No Hit
AGAGCCGC17478870.41658229515509615No Hit
CCGGTAGG17329410.41302014326347614No Hit
CTGTCATT17321190.41282423205948093No Hit
CAACGTAA17144140.4086045144600475No Hit
CATTCCAG17090910.4073358583300398No Hit
ACATTCCT16499610.39324312172148285TruSeq Adapter, Index 27 (100% over 8bp)
GCGAGATA16387170.3905632852522352No Hit
TGTTGCGT15918920.37940325833365446No Hit
CGCATATA15907880.37914013671660984No Hit
GCGCTGGC15667740.3734167648762938No Hit
GTAGCACA15619920.37227704787203003No Hit
TCGCACGC14933350.35591369564247316No Hit
CCGTACAC14807310.3529097238478807No Hit
ACACTGTG14696850.35027707766864646No Hit
TAACGGTC14624240.34854652870002256No Hit
GGAATATC14595480.3478610778208375RNA PCR Primer, Index 37 (100% over 8bp)
ATGGCGCT14526220.3462103710095596No Hit
TTACAGCG14226890.33907629549959956No Hit
AGGAATAC13754900.327827131366549No Hit
ACAGGCTG13329580.31769027573598674No Hit
AGGCCAGA12668430.301932770563067No Hit
CCGAATAG12554560.2992188522176985No Hit
AAGCCTGT12490040.29768111610069514No Hit
AGTGGATG12223540.29132949373272554No Hit
GTCGAAGC11014560.2625152933183619No Hit
CACATTAT10727880.2556827113279321No Hit
ACCTCGCA9991300.23812744677333902No Hit
TGCTAACT9859900.23499572752699302No Hit
TAGCGCTG9130390.2176089656746196No Hit
GCACTCTC9065600.21606479451806893No Hit
CCATTGAC7916100.1886682094824927No Hit
AGAAGTAG7621730.18165234803236427No Hit
CTTCTAGA6716540.1600785204478899No Hit
CTCTCGAA5949960.14180825149915388No Hit
AACGTCGA4963580.11829938368933073No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)