FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590250
Sequences flagged as poor quality0
Sequence length126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGAAAATCTCGTA132670.834271340984122Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG53620.33717968872818743Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA25460.16010061311114604Illumina Paired End Adapter 2 (100% over 32bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT17390.10935387517685899Illumina Single End PCR Primer 1 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)