FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001836653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530153
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGGCCATCTCGTA116672.2006854624985617Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG43500.8205178505073064Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA20240.38177658147742255Illumina Paired End Adapter 2 (100% over 32bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT17420.32858438978936266Illumina Single End PCR Primer 1 (96% over 29bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC9970.18805891884041023No Hit
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACAGATCGGA9090.17145993703704404Illumina Single End PCR Primer 1 (95% over 22bp)
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7430.14014822136251234No Hit
GCACCTCTTCCAGACCATCAGATCGGAAGAGCGGTTCAGCAGGAATGCCG7230.13637572549811094Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC7010.13222598004726938Illumina Paired End Adapter 2 (96% over 28bp)
AAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGCAGCAAAA5620.10600713378967959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)