FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001836654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530153
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG131112.4730596639083435Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT13740.25917046588437676Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT11910.2246521287251039Illumina Paired End Adapter 2 (96% over 30bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA11180.21088251882003875No Hit
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC11140.21012801964715846No Hit
GATGGTCTGGAAGAGGTGCCGATCGGAAGAGCGTCGTGTAGGGAAAGAGT8660.163349070928581Illumina Single End PCR Primer 1 (96% over 31bp)
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7150.13486672715235035No Hit
GCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC6930.1307169817015088No Hit
AAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGCAGCAAAA6380.12034261807440494No Hit
GTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAA6270.11826774534898417Illumina Single End PCR Primer 1 (100% over 40bp)
GGCGAGATGACAAGCTCCAACGTGAACGGACAGGATGCAGCAAAATGAGA6000.11317487593204226No Hit
AGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATG5920.11166587758628169No Hit
AGAGGCGAGATGACAAGCTCCAACGTGAACGGACAGGATGCAGCAAAATG5680.107138882549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)