FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678245
Sequences flagged as poor quality0
Sequence length126
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTA134331.9805527501124225Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG42590.6279441794631733Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA20500.3022506616340703Illumina Paired End Adapter 2 (100% over 32bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT12260.18076063959188787Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)