FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678245
Sequences flagged as poor quality0
Sequence length126
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG150492.218814735088353Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT14250.2101010696724635Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT11210.16527950814233794Illumina Paired End Adapter 2 (96% over 30bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA7310.10777816275829531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)