FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001836673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507917
Sequences flagged as poor quality0
Sequence length126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTA159101.0550978601607384Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG56680.37588275747272565Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA28090.18628346255132078Illumina Paired End Adapter 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)