FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685502
Sequences flagged as poor quality0
Sequence length126
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT11260.16425918523943037Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG9580.13975159809891147Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAATGCATCTCGTA8700.12691429054911585Illumina Paired End PCR Primer 2 (97% over 37bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC8320.12137090774352226No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7380.1076583292244224No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)