FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641709
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG12680.19759735331746942Illumina Paired End PCR Primer 2 (97% over 36bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT12540.19541567906948476Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTA10970.17094976071708515Illumina Paired End PCR Primer 2 (97% over 37bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC9990.1556780409811924No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8390.13074462100422465No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)