FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001836893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447174
Sequences flagged as poor quality0
Sequence length126
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG31430.2171819007251374Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACATCTATCTCGTA27620.19085472790417737Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA14640.10116267981597236Illumina Paired End Adapter 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)