FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646846
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG12650.1955643228836539Illumina Paired End PCR Primer 2 (97% over 36bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT12140.18767991144723783Illumina Single End PCR Primer 1 (96% over 29bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC10430.1612439436898427No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTA10250.15846121024169585Illumina Paired End PCR Primer 2 (97% over 37bp)
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8470.13094306836557698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)