FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646846
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC11730.18134146303756998No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11650.1801046926161714Illumina Single End PCR Primer 1 (100% over 50bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA8440.1304792794575525No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8110.12537760146928326No Hit
GCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC7410.1145558602820455No Hit
GATGGTCTGGAAGAGGTGCCGATCGGAAGAGCGTCGTGTAGGGAAAGAGT7220.11161853053122382Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)