FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001836981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001836981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813484
Sequences flagged as poor quality0
Sequence length126
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTA174372.143496368705469Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG53560.6584026237762513Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA26110.3209651326885347Illumina Paired End Adapter 2 (100% over 32bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT19460.23921798093140123Illumina Single End PCR Primer 1 (96% over 29bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC11140.13694184519916802No Hit
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACAGATCGGA10930.13436035619631118Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC9650.11862556608365991Illumina Paired End Adapter 2 (96% over 28bp)
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8940.10989767469304866No Hit
GCACCTCTTCCAGACCATCAGATCGGAAGAGCGGTTCAGCAGGAATGCCG8270.10166149549345777Illumina Paired End PCR Primer 2 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)