FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639019
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12390.19389094846945082Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC11500.17996335007253306No Hit
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA8560.1339553284018159No Hit
GCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC7990.12503540583300338No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7340.11486356430716457No Hit
GATGGTCTGGAAGAGGTGCCGATCGGAAGAGCGTCGTGTAGGGAAAGAGT7280.11392462508939484Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)