FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938548
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG846549.019677203510103Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT102571.0928583301013908Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT83300.8875411806322107Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA34190.36428611003379685Illumina Paired End Adapter 2 (96% over 29bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT12070.1286029057650754Illumina Single End PCR Primer 1 (100% over 31bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA10320.10995708264254998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)