FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863559
Sequences flagged as poor quality0
Sequence length126
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG14270.1652463815442836Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTACGATCTCGTA10070.1166104458409906Illumina Paired End PCR Primer 2 (97% over 36bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT9700.11232585150522431Illumina Single End PCR Primer 1 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)