FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755202
Sequences flagged as poor quality0
Sequence length126
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTACGATCTCGTA275573.6489574974642545Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG94691.2538367218307156Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA46390.6142727376251652Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC16230.21490938848149235Illumina Paired End Adapter 2 (96% over 28bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT11750.15558751168561524Illumina Single End PCR Primer 1 (96% over 29bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACAGATCGGA8290.10977195505308514Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)