FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755202
Sequences flagged as poor quality0
Sequence length126
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG322134.265481288449978Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT34340.45471277883268313Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT29680.393007433772686Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA13910.18418913085505598Illumina Paired End Adapter 2 (96% over 29bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA9020.11943824301312762No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)