FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741887
Sequences flagged as poor quality0
Sequence length126
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG144051.9416703621980165Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT14930.2012435856134425Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT12730.17158947386866194Illumina Paired End Adapter 2 (96% over 30bp)
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA8250.11120291904292703No Hit

[WARN]Adapter Content

Adapter graph