FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001837345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454787
Sequences flagged as poor quality0
Sequence length126
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTA37330010.805297113830752Illumina Paired End PCR Primer 2 (97% over 36bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTAT1737605.029543065896682Illumina Paired End PCR Primer 2 (97% over 35bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCGATCGGAAGAGCGGTTCA1378923.9913314482195283Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCGATCGGAAGAGCGTCGTG1304573.7761228116234085Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATCTCGTATGCCG903212.614372463483277Illumina Paired End PCR Primer 2 (96% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTGATCGGAAGAGCGGTTCA733362.1227357866056575Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGATCGGAAGAGCGGTTCAGC457551.3243942390659684Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGGATCGGAAGAGCGGTTCAGCA451881.307982228716271Illumina Paired End Adapter 2 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCGTCGT380781.1021808290930815Illumina Single End PCR Primer 1 (100% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGATCGGAAGAGCGGTTCAGCAG338250.979076278798085Illumina Paired End Adapter 2 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCGGAAGAGCGGTTCAGCAG308050.8916613383111607Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGGATCGGAAGAGCGGTTCAG280970.8132773453182498Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTGATCGGAAGAGCGGTTCAG264460.7654885814957623Illumina Paired End Adapter 2 (96% over 26bp)
GATCGGAAGAGCGTCGTGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGA242850.7029376919619068Illumina Paired End PCR Primer 2 (97% over 34bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGGATCGGAAGAGCGGTTCAGCAG240940.6974091311562768Illumina Paired End Adapter 2 (96% over 26bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACAACTCGTAT231430.6698821085062552Illumina Paired End PCR Primer 2 (97% over 35bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCGATCGGAAGAGCGTCGTGA205230.5940453058321685Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCGGTTCAGC193190.5591951110155271Illumina Single End PCR Primer 1 (96% over 30bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGTGATCGGAAGAGCGGTTCAGCA190290.5508009611012199Illumina Paired End Adapter 2 (96% over 26bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGATCGGAAGAGCGGTTCAGCAGG180840.5234476105183908Illumina Paired End Adapter 2 (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACAC179920.5207846388214382Illumina Single End PCR Primer 1 (100% over 33bp)
GCACTCTTTCCCTACACGACGCTCTTCCGATCGGAAGAGCGGTTCAGCAG162270.46969610572229203Illumina Single End PCR Primer 1 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATGATCGGAAGAGCGGTTCAG143450.4152209673128908Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCACACTCTTTCCCTACACG109340.3164883971139176Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGACACTCTTTCCCTACACGACG106780.3090783889137015Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGTGGATCGGAAGAGCGGTTCAGC104580.30271041311664076Illumina Paired End Adapter 2 (96% over 26bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGATCGGAAGAGCGGTTCAGCAGGA103130.2985133381594871Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGATCGGAAGAGCGGTTCAGCAGGAA99510.288035123438869Illumina Paired End Adapter 2 (96% over 26bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC97820.2831433602129451Illumina Paired End PCR Primer 2 (97% over 35bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTGATCGGAAGAGCGTCGTG95130.2753570625338118Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTACACTCTTTCCCTACACG93960.2719704572235568Illumina Paired End Adapter 2 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCGTCGTGAT85030.24612226455639669Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCGGAAGAGCGTCGTGATCG83030.24033319564997785Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAGCGGTTCAGCAGGAAAGAGTGTGATCGGAAGAGCGGTTCAGCAGG80060.2317364283239459Illumina Paired End PCR Primer 2 (95% over 24bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA78810.22811826025743412Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG64900.18785528601329113Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGGATCGGAAGAGCGGTTCAGCAGG63310.18325297623268816Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAAAAGAGTGTGATCGGAAGAGCGGTTC63200.18293457744283512Illumina Paired End Adapter 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGACACTCTTTCCCTACACGA61230.17723234457001255Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGAGACACTCTTTCCCTACACGACGC58390.16901186672289784Illumina Paired End Adapter 2 (96% over 26bp)
CACTCTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGGTTCA57650.16686991122752284Illumina Paired End Adapter 2 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCACACTCTTTCCCTACACGA57630.16681202053845867Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCTACACTCTTTCCCTACACG57480.16637784037047726Illumina Single End PCR Primer 1 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGGATCGGAAGAGCGTCGTGATC57120.16533580796732186Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGGATCGGAAGAGCGGTTCAGCAGGAATG55260.15995197388435234Illumina Paired End PCR Primer 2 (96% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATGATCGGAAGAGCGTCGTGA53840.15584173496079498Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGCTACATATCGTAT53530.15494442928030006Illumina Paired End PCR Primer 2 (97% over 35bp)
ATCGGAAGAGCGGTTCAGCAGGGATCGGAAGAGCGGTTCAGCAGGAATGC50790.14701340487850628Illumina Paired End PCR Primer 2 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTACACTCTTTCCCTACACGAC46570.13479846948596252Illumina Paired End Adapter 2 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCGGTTCAGCA46400.13430639862891694Illumina Single End PCR Primer 1 (96% over 29bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAG44760.12955936212565347Illumina Paired End Adapter 2 (100% over 31bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCTGATCGGAAGAGCGTCGTG44680.12932779936939673Illumina Single End PCR Primer 1 (100% over 32bp)
GAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGG38040.1101080906000862Illumina Paired End Adapter 2 (100% over 27bp)
ATCGGAAGAGCGGTTCAGCAGGAAAGGATCGGAAGAGCGGTTCAGCAGGA38010.11002125456648992Illumina Paired End Adapter 2 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)