FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001837346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454787
Sequences flagged as poor quality0
Sequence length126
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3126369.049356733135792Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1580914.575998462423298Illumina Single End PCR Primer 1 (100% over 50bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA967182.799535832455083Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTG495781.4350522912121644Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCGGAAGAGCGTCGTG483611.3998258069166059Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGG470531.3619652962686266Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTG356241.0311489536113225Illumina Paired End Adapter 2 (96% over 27bp)
TCTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAG343240.9935200057196001Illumina Paired End Adapter 2 (100% over 25bp)
CTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG300850.8708206902480529Illumina Paired End Adapter 2 (96% over 27bp)
TCTTTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTAGG288900.8362310035322004Illumina Paired End Adapter 2 (100% over 25bp)
CACTCTTTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGT251590.728235923082957Illumina Paired End Adapter 2 (96% over 27bp)
ACTCTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT241500.699030070450074Illumina Paired End Adapter 2 (100% over 25bp)
CTTTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTAGGG236980.6859467747215675Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT168120.4866291322735671Illumina Single End PCR Primer 1 (98% over 50bp)
CTCTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA166700.4825188933500097Illumina Paired End Adapter 2 (96% over 27bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA166400.4816505330140469Illumina Single End PCR Primer 1 (97% over 34bp)
TTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTAGGGAA150290.4350195829728432Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGT148340.42937524078908484Illumina Single End PCR Primer 1 (97% over 37bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT142780.4132816292292405Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTGATCGGAAGAGCGTCGTG139010.402369234340641Illumina Paired End Adapter 2 (96% over 27bp)
ACTCTTTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTA137890.3991273557530465Illumina Paired End Adapter 2 (100% over 25bp)
CTCTTTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTAG136460.39498817148495696Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTTTCCTGCTGAACCG111750.3234642251461523Illumina Single End PCR Primer 1 (96% over 32bp)
AGGCATTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTA100860.2919427449507017Illumina Paired End Adapter 2 (96% over 30bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGT97820.2831433602129451Illumina Single End PCR Primer 1 (97% over 35bp)
TTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGA94550.2736782325509503Illumina Single End PCR Primer 1 (100% over 25bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA86640.2507824650260638Illumina Single End PCR Primer 1 (100% over 50bp)
CTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTAGGGAAAGA85050.24618015524546086Illumina Single End PCR Primer 1 (96% over 29bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCGATCGGAAGAGCGTCGTGTA74380.21529547262971638Illumina Paired End Adapter 2 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCGGAAGAGCGTCGTGT69060.1998965493386423Illumina Single End PCR Primer 1 (96% over 33bp)
CACTCTTTCCTGCTGAACCGCTCTTCCGATCACACTCTTTCCTGCTGAAC62620.18125574745997364Illumina Paired End Adapter 2 (96% over 27bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTGATCGGAAGAGCGTCGTGTAGGG61070.17676921905749907Illumina Single End PCR Primer 1 (96% over 27bp)
ACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT60610.17543773320902273Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACACTCTTTCCTGCTGAA58110.16820139707599918Illumina Single End PCR Primer 1 (97% over 35bp)
CTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAG57560.166609403126734Illumina Single End PCR Primer 1 (100% over 28bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGGAAGAGCGTCGTGTA54710.15835997993508716Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACTCTTTCCTGCTGAAC52690.15251302033960415Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCTGCTGAACCGCTCTTCCGATCGGAAGAGCGTCGTGTAGGG50570.1463766072988002Illumina Paired End Adapter 2 (96% over 27bp)
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCGGAAGAGCGTCGTGTAG45120.13060139452880887Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAAAGAGTGTAGATCGGAAGAGCGTCGT42910.12420447338721606Illumina Paired End Adapter 2 (96% over 27bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCGGAAGAGCGTCGTG41800.1209915401441536Illumina Single End PCR Primer 1 (97% over 36bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCGGAAGAGCGTCGTGT41340.11966005429567728Illumina Single End PCR Primer 1 (96% over 31bp)
CACTCTTTTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGT40010.11581032347290875Illumina Paired End Adapter 2 (96% over 26bp)
TCTTTCCTGCTGAACCGCTCTTCCGATCACACTCTTTCCCTACACGACGC39780.11514458054867058Illumina Paired End Adapter 2 (100% over 25bp)
CTTTCCTGCTGAACCGCTCTTCCGATCACACTCTTTCCTGCTGAACCGCT38920.11265528091891049Illumina Paired End Adapter 2 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTCTTTCCTGCTGAACC38060.1101659812891504Illumina Single End PCR Primer 1 (96% over 33bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG36990.10706882942421632Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)