FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639084
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT12610.19731365516896057Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG12580.19684423330892342Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTA10310.1613246458994436Illumina Paired End PCR Primer 2 (97% over 37bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC10040.157099849159109No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8690.13597586545743595No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA6420.10045627804795612Illumina Paired End Adapter 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)