FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001837422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639084
Sequences flagged as poor quality0
Sequence length126
%GC60

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11850.1854216347146854Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC11340.17744146309405337No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG8430.13190754267044708No Hit
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA8260.12924748546356973No Hit
GCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC8020.1254921105832723No Hit
GATGGTCTGGAAGAGGTGCCGATCGGAAGAGCGTCGTGTAGGGAAAGAGT7360.11516482966245438Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)