FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001837475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570621
Sequences flagged as poor quality0
Sequence length126
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG12000.21029720252146344Illumina Paired End PCR Primer 2 (97% over 36bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACACGACGCT11540.2022358097581407Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTGAATCTCGTA11020.19312293098221062Illumina Paired End PCR Primer 2 (97% over 37bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC9830.17226845839883215No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7730.13546644795757604No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA5900.10339612457305287Illumina Paired End Adapter 2 (100% over 32bp)
GCACCTCTTCCAGACCATCAAGGATCTACACTCTTTCCCTACAGATCGGA5750.10076740954153458Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)