FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001837476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001837476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570621
Sequences flagged as poor quality0
Sequence length126
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12250.2146783942406606Illumina Single End PCR Primer 1 (100% over 50bp)
GCTGGGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC10250.17962886048708337No Hit
GTAGGGAAAGAGTGTAGATCCTTGATGGTCTGGAAGAGGTGCCGATCGGA7680.1345902096137366No Hit
GAACCGAATAGCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACG7490.13126050390714677No Hit
GCCGAGAAAAGGTAGAAGATGACAAGCTCCAACGTGAACGGACAGGATGC7010.12284861580628824No Hit
GATGGTCTGGAAGAGGTGCCGATCGGAAGAGCGTCGTGTAGGGAAAGAGT6480.11356048936159027Illumina Single End PCR Primer 1 (96% over 31bp)
GTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAA6010.10532384892949961Illumina Single End PCR Primer 1 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)