FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14477710
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC7876945.440736138519144TruSeq Adapter, Index 12 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4798223.3142119851827396No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT3585262.476399927889148TruSeq Adapter, Index 12 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG1829251.2634940194271054TruSeq Adapter, Index 12 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1829071.2633696903723033Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1789611.236113998691782Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1629261.1253575323721776No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1248230.8621736448651064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC771400.5328190715244331Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT644060.44486317242160534TruSeq Adapter, Index 12 (100% over 38bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG643810.44469049317882453TruSeq Adapter, Index 12 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC565810.3908145694312153Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA425800.2941072863042567TruSeq Adapter, Index 12 (100% over 39bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG424650.29331296178746497TruSeq Adapter, Index 12 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC407550.28150170158125837No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT338660.23391820944058142TruSeq Adapter, Index 12 (100% over 51bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT332110.2293940132797245TruSeq Adapter, Index 12 (100% over 38bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC329030.22726660500866505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG312490.2158421463062874TruSeq Adapter, Index 12 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT274590.18966397310071828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT259490.17923414683675803TruSeq Adapter, Index 12 (100% over 36bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC253450.17506221633117391Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC250730.17318346616971883No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT244590.1689424639670224Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT244580.16893555679731118No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA237950.16435610327876438Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC232360.16049499541018572No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG231390.1598249999481962Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG226970.1567720309358317TruSeq Adapter, Index 12 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT226030.1561227569829759No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC212620.14686024240021384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC211520.14610045373197833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT196050.1354150621887025TruSeq Adapter, Index 1 (97% over 35bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT183730.12690542910446473TruSeq Adapter, Index 12 (100% over 36bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT176930.122208553700827TruSeq Adapter, Index 1 (97% over 35bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT175270.12106196352876249TruSeq Adapter, Index 12 (100% over 36bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG169090.11679333264722114TruSeq Adapter, Index 12 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG165570.1143620089088675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT162740.11240727988058885TruSeq Adapter, Index 12 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT160160.11062523009509101Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG156260.10793143390771054TruSeq Adapter, Index 12 (100% over 37bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA155540.10743411768850185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT155250.10723380976687613TruSeq Adapter, Index 12 (100% over 38bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA150360.10385620377808369TruSeq Adapter, Index 12 (100% over 40bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA146130.10093447099023256Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)