FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20261066
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC15322937.56274620496276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5111042.5225918517811454No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3059881.5102265596489346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1396850.6894257192587991Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1185700.5852110644128992TruSeq Adapter, Index 1 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1081030.5335504064791062TruSeq Adapter, Index 1 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1012420.4996874300690793Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT818120.4037892181980948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA766480.3783019116565733TruSeq Adapter, Index 1 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC721550.3561263755816204No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC667370.3293854331257793TruSeq Adapter, Index 1 (100% over 50bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA612590.3023483562019886Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA607020.2995992412245239Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC556860.27484239970394453TruSeq Adapter, Index 1 (100% over 38bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG508570.2510085106084744TruSeq Adapter, Index 1 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT449010.22161222909001924No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT446090.22017104134599827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG406930.20084333173782665Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA362180.17875663600325867TruSeq Adapter, Index 1 (100% over 37bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC334300.1649962543925379TruSeq Adapter, Index 1 (100% over 36bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC328420.16209413660663263No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT325710.160756595926394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG313660.15480922869507457Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA311560.15377275805725127TruSeq Adapter, Index 1 (100% over 37bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC292430.1443310041041276Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC281460.1389166789151173TruSeq Adapter, Index 1 (100% over 38bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA260410.12852729466455518Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA255350.1260298939848476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA241060.11897695807318331TruSeq Adapter, Index 1 (100% over 40bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC237610.11727418488247361Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA228140.1126001958633371TruSeq Adapter, Index 1 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC226470.11177595492754429Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC224750.11092703611942235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)